Results 91 to 100 of about 6,315 (155)

Gated rotation mechanism of site-specific recombination by ϕC31 integrase [PDF]

, 2012
Integrases, such as that of the Streptomyces temperate bacteriophage ϕC31, promote site-specific recombination between DNA sequences in the bacteriophage and bacterial genomes to integrate or excise the phage DNA.
Stark, W. Marshall, Rosser, Susan J., Olorunniji, Femi J., Colloms, Sean D., McEwan, Andrew R., Smith, Margaret C. M., Buck, Dorothy E.   +6 more
core   +1 more source

Multi-target QSAR modelling in the analysis and design of HIV-HCV co-inhibitors: an in-silico study

BMC Bioinformatics, 2011
Background HIV and HCV infections have become the leading global public-health threats. Even more remarkable, HIV-HCV co-infection is rapidly emerging as a major cause of morbidity and mortality throughout the world, due to the common rapid mutation ...
Liu Qi, Zhou Han, Liu Lin, Chen Xi, Zhu Ruixin, Cao Zhiwei   +5 more
doaj   +1 more source

Engineering Conditional Transgene Expression in Nicotiana benthamiana

Plant Biotechnology Journal, Volume 24, Issue 1, Page 18-30, January 2026.
ABSTRACT Nicotiana benthamiana has emerged as a premier plant biofactory for recombinant protein and metabolite production due to its high metabolic versatility, ease of cultivation and permissiveness to transient expression vectors. However, challenges such as transgene silencing, low yields and metabolic toxicity limit its scalability. Synthetic gene
Elena Garcia‐Perez, Marta Vazquez‐Vilar, Diego Orzaez   +2 more
wiley   +1 more source

Excision dynamics of Vibrio pathogenicity island-2 from Vibrio cholerae: role of a recombination directionality factor VefA

BMC Microbiology, 2010
Background Vibrio Pathogenicity Island-2 (VPI-2) is a 57 kb region present in choleragenic V. cholerae isolates that is required for growth on sialic acid as a sole carbon source. V.
Napolitano Michael G, Almagro-Moreno Salvador, Boyd E   +2 more
doaj   +1 more source

Health Benefits and Therapeutic Potential of Quercetin

Journal of Food Biochemistry, Volume 2026, Issue 1, 2026.
Quercetin, a polyphenolic compound prevalent in many fruits, vegetables, and other dietary sources, has attracted considerable interest due to its extensive health benefits. This review examines its diverse biological properties, particularly its antioxidant and anti‐inflammatory effects. The study integrates recent scientific findings to elucidate the
Mahendra Aryal, Kim Wei Chan
wiley   +1 more source

Recombination directionality factor gp3 binds ϕC31 integrase via the zinc domain, potentially affecting the trajectory of the coiled-coil motif [PDF]

, 2017
To establish a prophage state, the genomic DNA of temperate bacteriophages normally becomes integrated into the genome of their host bacterium by integrase-mediated, site-specific DNA recombination.
Stark, W. Marshall, Paul C M Fogg, Fernando, Booshini D., Khaleel, Thanafez, Smith, Margaret C M, Thanafez Khaleel, W Marshall Stark, Younger, Ellen, Margaret C M Smith, Ellen Younger, Fernando, Booshini D, Booshini D Fernando, Smith, Margaret C.M., Fogg, Paul C.M., Fogg, Paul C M, Stark, W Marshall   +15 more
core   +1 more source

Structural basis of directionality control in large serine integrases

Summary Large serine integrases (LSIs) catalyze unidirectional site-specific insertion of large DNA payloads, and in the presence of a cognate recombination directionality factor (RDF), catalyze unidirectional excision. Because neither reaction changes the net number of covalent bonds, the preferred direction must be controlled by the ...
Heewhan Shin, Ying Pigli, Tania Peña Reyes, Adebayo J. Bello, James R. Fuller, Femi J. Olorunniji, Phoebe A. Rice   +6 more
openaire   +2 more sources

Single copy optogenetic system for Streptomyces

Scientific Reports
LitR is a blue-green light-sensing transcriptional regulator that uses coenzyme B12 as a chromophore. In this study, we developed a genome-integrative light-inducible expression (iLiEX) system in Streptomyces griseus NBRC 13350, a Gram-positive bacterium
Airi Watanabe, Ryuta Noya, Rio Yamada, Hideaki Takano   +3 more
doaj   +1 more source

The reaction directionality mechanism of \u3d5C31 integrase with recombination directionality factor

, 2023
Serine integrases catalyse a site-specific recombination between an attachment site in the host (attB) and an attachment site in the phage(attP). The reverse reaction requires an additional protein, the recombination directionality factor (RDF ) to ...
Hsieh, Chih-Hui
core  

Isolation and characterization of the new Streptomyces phages Kamino, Geonosis, Abafar, and Scarif infecting a broad range of host species

Microbiology Spectrum
Streptomyces, a multifaceted genus of soil-dwelling bacteria belonging to the phylum Actinomycetota, features intricate phage-host interactions shaped by its complex life cycle and the synthesis of a diverse array of specialized metabolites.
Bente Rackow, Clara Rolland, Isabelle Mohnen, Johannes Wittmann, Mathias Müsken, Jörg Overmann, Julia Frunzke   +6 more
doaj   +1 more source