Results 31 to 40 of about 6,315 (155)
Large serine integrases utilise scavenged phage proteins as directionality cofactors [PDF]
Nucleic Acids ResearchAbstract Recombination directionality factors (RDFs) for large serine integrases (LSIs) are cofactor proteins that control the directionality of recombination to favour excision over insertion. Although RDFs are predicted to bind their cognate LSIs in similar ways, there is no overall common structural theme across LSI RDFs, leading ...
Abdulrazak Alsaleh +8 more
core +4 more sources
Development of circular AAV cargos for targeted seamless insertion with large serine integrases
Molecular Therapy: Methods & Clinical DevelopmentRecent advancements in gene insertion have shifted from DNA-repair-dependent mechanisms to more precise approaches, enhancing safety and predictability for editing outcomes. Integrase-mediated programmable genomic integration (I-PGI) utilizes a DNA cargo
Brett J.G. Estes +12 more
doaj +3 more sources
Control of the Serine Integrase Reaction: Roles of the Coiled-Coil and Helix E Regions in DNA Site Synapsis and Recombination. [PDF]
J Bacteriol, 2021 Bacteriophage serine integrases catalyze highly specific recombination reactions between defined DNA segments called att sites. These reactions are reversible depending upon the presence of a second phage-encoded directionality factor.
Mandali S, Johnson RC.
europepmc +2 more sources
Virology, 2003 L-chicoric acid (L-CA) is a potent inhibitor of HIV integrase (IN) in vitro. In this report, the effects of a glycine to serine mutation at position 140 (G140S) on HIV IN and its effects on IN inhibitor resistance are described. HIV containing the G140S mutation showed a delay in replication.
King, Peter J +5 more
openaire +3 more sources
Cross-talk between Diverse Serine Integrases [PDF]
Journal of Molecular Biology, 2014 Phage-encoded serine integrases are large serine recombinases that mediate integrative and excisive site-specific recombination of temperate phage genomes. They are well suited for use in heterologous systems and for synthetic genetic circuits as the attP and attB attachment sites are small (
Shweta, Singh +4 more
openaire +2 more sources
Genetic switches designed for eukaryotic cells and controlled by serine integrases [PDF]
Communications Biology, 2020 AbstractRecently, new serine integrases have been identified, increasing the possibility of scaling up genomic modulation tools. Here, we describe the use of unidirectional genetic switches to evaluate the functionality of six serine integrases in different eukaryotic systems: the HEK 293T cell lineage, bovine fibroblasts and plant protoplasts ...
Mayna S. Gomide +17 more
openaire +2 more sources
Attachment site recognition and regulation of directionality by the serine integrases [PDF]
Nucleic Acids Research, 2013 Abstract Serine integrases catalyze the integration of bacteriophage DNA into a host genome by site-specific recombination between ‘attachment sites’ in the phage ( attP ) and the host ( attB ). The reaction is highly directional; the reverse excision reaction between the product attL and attR sites does not occur in the absence of a ...
Rutherford, Karen +4 more
openaire +2 more sources
PLoS ONE, 2022 The threat to public health posed by drug-resistant bacteria is rapidly increasing, as some of healthcare's most potent antibiotics are becoming obsolete.
Elizabeth T Montaño +40 more
doaj +2 more sources
Control of phage Bxb1 excision by a novel recombination directionality factor. [PDF]
PLoS Biology, 2006 Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site and a phage-encoded integrase generating the recombinant junctions attL and attR.
Pallavi Ghosh +2 more
doaj +1 more source
, 2021 This deposit contains the data, code, analysis, and sequence files for KeHuan Edmonds's thesis, "Chapter 3: Building serine-integrase based, image-readable recording systems".
Yoon, Shinae +4 more
core +1 more source