Mathematical modelling of serine integrase - mediated gene assembly [PDF]
bioRxiv, 2018 Site-specific recombination promoted by serine integrases can be used for ordered assembly of DNA fragments into larger arrays. When a plasmid vector is included in the assembly, the circular product DNA molecules can transform E. coli cells.
A. Pokhilko +3 more
semanticscholar +2 more sources
The site-specific integration reaction of
Mobile DNA, 2013 Background A large subfamily of serine recombinases contains long polypeptide segments appended to the C-terminal end of the conserved catalytic domain.
Mandali Sridhar +4 more
doaj +5 more sources
A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome [PDF]
Applied Microbiology and BiotechnologyPhages are a valuable resource for the genetic engineering of Streptomyces antibiotic-producing bacteria. Indeed, a few integrative vectors based on phage integrase are available to insert transgenes at specific genomic loci.
Nicolas Delhaye +5 more
doaj +2 more sources
Heterologous Substitution of Mycobacterium tuberculosis rRNA in Mycobacterium smegmatis and Its Impact on Antimicrobial Susceptibility [PDF]
AntibioticsBackground: The global incidence of multidrug-resistant and extensively drug-resistant tuberculosis continues to rise. The ribosome serves as a target for multiple antimicrobials, making functional research on it hold great significance.
Qianwen Yue +4 more
doaj +2 more sources
Multi-target QSAR modelling in the analysis and design of HIV-HCV co-inhibitors: an in-silico study [PDF]
BMC Bioinformatics, 2011 Background HIV and HCV infections have become the leading global public-health threats. Even more remarkable, HIV-HCV co-infection is rapidly emerging as a major cause of morbidity and mortality throughout the world, due to the common rapid mutation ...
Liu Qi +5 more
doaj +4 more sources
Large Serine Integrase Off-target Discovery and Validation for Therapeutic Genome Editing
bioRxivWhile numerous technologies for the characterization of potential off-target editing by CRISPR/Cas9 have been described, the development of new technologies and analytical methods for off-target recombination by Large Serine Integrases (LSIs) are ...
Dane Z. Hazelbaker +22 more
semanticscholar +2 more sources
Identification of cognate recombination directionality factors for large serine recombinases by virtual pulldown. [PDF]
Nucleic Acids ResIntegrases from the “large serine” family are simple, highly directional site-specific DNA recombinases that have great promise as synthetic biology and genome editing tools.
Shin H +11 more
europepmc +2 more sources
Highly enhanced transgene integration in human pluripotent stem cells by serine integrase BxbI
bioRxivGenome engineering and especially integration of large DNA payloads in human pluripotent stem cells (hPSC) remains challenging largely due to the sensitive nature of hPSCs.
Matias I. Autio, A. Perrin
semanticscholar +2 more sources
A history-dependent integrase recorder of plant gene expression with single-cell resolution
Nature CommunicationsDuring development, most cells experience a progressive restriction of fate that ultimately results in a fully differentiated mature state. Understanding more about the gene expression patterns that underlie developmental programs can inform engineering ...
Cassandra J. Maranas +5 more
doaj +2 more sources
Serine Integrase-based Recombination Enables Direct Plasmid Assembly In Vivo
Synthetic Biology and Engineering, 2023 Luyao Wang +4 more
semanticscholar +2 more sources