Results 131 to 140 of about 98,107 (317)

Tissue-selective expression of a conditionally-active ROCK2-estrogen receptor fusion protein [PDF]

, 2016
The serine/threonine kinases ROCK1 and ROCK2 are central mediators of actomyosin contractile force generation that act downstream of the RhoA small GTP-binding protein. As a result, they have key roles in regulating cell morphology and proliferation, and
Boyle, Sarah T., Bryson, Sheila, Greenhalgh, David A., Kochetkova, Marina, Masre, Siti F., Olson, Michael F., Rath, Nicola, Samuel, Michael S., Stevenson, David   +8 more
core   +1 more source

Astrocytic TIA1‐Mediated Stress Granules Promote the Demyelination of Optic Neuritis by Sequestering mRNA of Cholesterol Synthesis Genes in an Experimental Autoimmune Encephalomyelitis Model

Advanced Science, EarlyView.
Astrocytic TIA1‐mediated stress granules (SGs) promote demyelination by sequestering the mRNA of cholesterol synthesis genes. In this model, astrocytic TIA1‐mediated SGs were increased in the optic nerves of EAE mice, leading to the downregulation of cholesterol synthesis genes such as HMGCS1 through sequestration of their mRNA into SGs, which ...
Zheyu Fang, Xin Hua, Ziwei Fan, Jiaxin Zhao, Yuan Zhang, Qiaoyi Zhang, Xi Qu, Shengli Pan, Bingbing Wan, Shiyin Yang, Jia Li, Xu Zhang   +11 more
wiley   +1 more source

Expression of Cre recombinase in retinal Müller cells

Vision Research, 2009
In an effort to generate inducible RPE-specific Cre mice using a 3.0-kb human vitelliform macular dystrophy-2 (VMD2) promoter, we identified a mouse line with unanticipated Cre activity in the neural retina, including Müller glial cells. Müller cells play important roles in the function and maintenance of the retina, and this mouse line would be ...
Ueki, Yumi, Ash, John D., Zhu, Meili, Zheng, Lixing, Le, Yun-Zheng   +4 more
openaire   +2 more sources

Deletion of Cardiac miR-17-92 Cluster Increases Ischemia/ Reperfusion Injury via PTEN Upregulation [PDF]

, 2017
The miR-17- 92 cluster is necessary for cell proliferation and development of the cardiovascular system. Deletion of this cluster leads to death in neonatal mice.
Prakash, Meeta B
core   +1 more source

Persistently Increased Expression of PKMzeta and Unbiased Gene Expression Profiles Identify Hippocampal Molecular Traces of a Long‐Term Active Place Avoidance Memory and “Shadow” Proteins

Advanced Science, EarlyView.
Protein complexes like KIBRA‐PKMζ are crucial for maintaining memories, forming month‐long protein traces in memory‐tagged neurons, but conventional RNA‐seq analysis fails to detect their transcript changes, leaving memory molecules undetected in the shadows of abundantly‐expressed genes.
Jiyeon Han, Alejandro Grau‐Perales, Rayna M. Harris, Edith Lesburguères, Hsin‐Yi Kao, Asit Pal, Juan Marcos Alarcon, Todd C. Sacktor, Stefano Martiniani, Hans A. Hofmann, André A. Fenton   +10 more
wiley   +1 more source

Hemizygous Le-Cre transgenic mice have severe eye abnormalities on some genetic backgrounds in the absence of LoxP sites.

PLoS ONE, 2014
Eye phenotypes were investigated in Le-Cre(Tg/-); Pax6(fl/+) mice, which were expected to show tissue-specific reduction of Pax6 in surface ectoderm derivatives.
Natalie J Dorà, J Martin Collinson, Robert E Hill, John D West   +3 more
doaj   +1 more source

Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases

Scientific Reports, 2020
The tyrosine-type site-specific DNA recombinase Cre recombines its target site, loxP, with high activity and specificity without cross-recombining the target sites of highly related recombinases. Understanding how Cre achieves this precision is key to be
Anjali Soni, Martina Augsburg, Frank Buchholz, M. Teresa Pisabarro   +3 more
doaj   +1 more source

The actin binding proteins cortactin and HS1 are dispensable for platelet actin nodule and megakaryocyte podosome formation [PDF]

, 2016
A dynamic, properly organised actin cytoskeleton is critical for the production and haemostatic function of platelets. The Wiskott Aldrich Syndrome protein (WASp) and Actin-Related Proteins 2 & 3 Complex (Arp2/3 complex) are critical mediators of ...
Bem, Danai, Finney, Brenda, Machesky, Laura M., Poulter, Natalie S., Thomas, Steven G., Watson, Stephen P.   +5 more
core   +4 more sources

In Vivo CRISPR Screening Identifies the Glutamate Receptor GRIA2 as Promoting Peritoneal Metastasis of Gastric Cancer via Calcium‐Dependent β‐Catenin Activation

Advanced Science, EarlyView.
We identified GRIA2 as a critical driver of gastric cancer peritoneal metastasis through in vivo CRISPR screening. Mechanistically, GRIA2‐mediated calcium influx inhibits GSK3β and activates Wnt/β‐catenin signaling, driven by glutamate from cancer‐associated fibroblasts.
Jie Sun, Xu Yang, Wenchao Jiang, Chengbo Ji, Yingying Wu, Haoyu Sun, Xinyou Liu, Masami Yamamoto, Tetsuya Tsukamoto, Sachiyo Nomura, Junjie Zhao, Yuanyuan Ruan, Haojie Li, Xuefei Wang   +13 more
wiley   +1 more source

Generation and characterization of an Advillin-Cre driver mouse line

Molecular Pain, 2011
Progress in the somatosensory field has been restricted by the limited number of genetic tools available to study gene function in peripheral sensory neurons.
Moreira Pedro, Al Banchaabouchi Mumna, Hublitz Philip, Martínez Conception, Piszczek Agnieszka, Zurborg Sandra, Perlas Emerald, Heppenstall Paul A   +7 more
doaj   +1 more source