Cre recombinase affects calcium dynamics already in young mice. [PDF]
Front PharmacolLiffers JL +5 more
europepmc +3 more sources
A Human CD68 Promoter-Driven Inducible Cre-Recombinase Mouse Line Allows Specific Targeting of Tissue Resident Macrophages. [PDF]
Front Immunol, 2022 Rumianek AN +4 more
europepmc +3 more sources
eLife, 2021 While Cre-dependent viral systems permit the manipulation of many neuron types, some cell populations cannot be targeted by a single DNA recombinase.
Paul V Sabatini +7 more
doaj +1 more source
Correction to 'Mechanisms of Cre recombinase synaptic complex assembly and activation illuminated by Cryo-EM'. [PDF]
Nucleic Acids Res, 2022 europepmc +3 more sources
Scientific Reports, 2021 We previously reported efficient precision targeted integration of reporter DNA in zebrafish and human cells using CRISPR/Cas9 and short regions of homology.
Maira P. Almeida +7 more
doaj +1 more source
Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons. [PDF]
PLoS ONE, 2012 To establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer), which expressed a tamoxifen inducible type of Cre recombinase specifically in ...
Mandy Ka Man Kam +3 more
doaj +1 more source
Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors. [PDF]
, 2020 The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver lines ...
Abumaria, Nashat +55 more
core +2 more sources
Generation of chickens expressing Cre recombinase [PDF]
Transgenic Research, 2016 Cre recombinase has been extensively used for genome engineering in transgenic mice yet its use in other species has been more limited. Here we describe the generation of transgenic chickens expressing Cre recombinase. Green fluorescent protein (GFP)-positive chicken primordial germ cells were stably transfected with β-actin-Cre-recombinase using ...
Leighton, Philip A. +6 more
openaire +2 more sources
Genomic targeting with purified Cre recombinase [PDF]
Nucleic Acids Research, 1993 Purified Cre recombinase protein introduced directly into cultured mammalian cells by lipofection catalyzes both site-specific chromosomal integration of a co-transfected lox targeting vector and precise excision of genomic DNA flanked by directly repeated lox sites.
W, Baubonis, B, Sauer
openaire +2 more sources
New Clox Systems for rapid and efficient gene disruption in Candida albicans [PDF]
, 2014 Acknowledgements: We are grateful to Janet Quinn, Lila Kastora, Joanna Potrykus, Michelle Leach, and others for sharing their experiences with the Clox cassettes.
Ballou, Elizabeth R +6 more
core +13 more sources