Unmodified Cre recombinase crosses the membrane [PDF]
Nucleic Acids Research, 2002 Site-specific recombination in genetically modified cells can be achieved by the activity of Cre recombinase from bacteriophage P1. Commonly an expression vector encoding Cre is introduced into cells; however, this can lead to undesired side-effects. Therefore, we tested whether cell-permeable Cre fusion proteins can be directly used for lox-specific ...
Elke, Will +7 more
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Dre - Cre sequential recombination provides new tools for retinal ganglion cell labeling and manipulation in mice. [PDF]
PLoS ONE, 2014 BACKGROUND: Genetic targeting methods have greatly advanced our understanding of many of the 20 Retinal Ganglion Cell (RGC) types conveying visual information from the eyes to the brain.
Szilard Sajgo +7 more
doaj +1 more source
40008 COMBINED CRISPR/CAS9 AND AAV FOR THE GENERATION OF CONDITIONAL ISOGENIC GENE KNOCK-INS
Journal of Clinical and Translational Science, 2021 IMPACT: We describe a novel methodology that combines CRISPR/Cas9-induced double stranded DNA breaks with homology dependent repair from an adeno associated virus (AAV) encoded template to generate single-allele edited isogenic cell line models of cancer-
Prajwal Boddu +3 more
doaj +1 more source
Distribution and characterisation of Glucagon-like peptide-1 receptor expressing cells in the mouse brain. [PDF]
, 2015 © 2015 The Authors.Objective: Although Glucagon-like peptide 1 is a key regulator of energy metabolism and food intake, the precise location of GLP-1 receptors and the physiological relevance of certain populations is debatable.
Cork, SC +5 more
core +2 more sources
Impact of E1 and Cre on adenovirus vector amplification: developing MDCK CAV-2-E1 and E1-Cre transcomplementing cell lines. [PDF]
PLoS ONE, 2013 Adenovirus vectors have been extensively studied through the manipulation of viral genome. However, little attention is being paid to their producer cell-lines; cells are selected according to virus yields, neglecting the expression profile of ...
Paulo Fernandes +6 more
doaj +1 more source
Mutants of Cre recombinase with improved accuracy [PDF]
Nature Communications, 2013 Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted towards use as transgene delivery vectors. The specificity of recombinases can be altered either with directed evolution or via fusions
Eroshenko, Nikolai, Church, George
openaire +3 more sources
Marker-free transgenic plants through genetically programmed auto-excision [PDF]
, 2007 We present here a vector system to obtain homozygous marker-free transgenic plants without the need of extra handling and within the same time frame as compared to transformation methods in which the marker is not removed.
Verweire, Dimitri +4 more
core +2 more sources
BioTechniques, 2010 We have established an in vitro Cre/loxP-based assay for monitoring cell fusion events that specifically traces the transport of cytoplasm from one cell to its fusion partner.
Kurt Pfannkuche +6 more
doaj +1 more source
Construction of Marker-Free Transgenic Strains of Chlamydomonas reinhardtii Using a Cre/loxP-Mediated Recombinase System. [PDF]
PLoS ONE, 2016 The Escherichia coli bacteriophage P1 encodes a site-specific recombinase called Cre and two 34-bp target sites of Cre recombinase called loxP. The Cre/loxP system has been used to achieve targeted insertion and precise deletion in many animal and plant ...
Yuki Kasai, Shigeaki Harayama
doaj +1 more source
An investigation of herpes simplex virus promoter activity compatible with latency establishment reveals VP16-independent activation of immediate-early promoters in sensory neurones [PDF]
, 2011 Herpes simplex virus (HSV) type-1 establishes lifelong latency in sensory neurones and it is widely assumed that latency is the consequence of a failure to initiate virus immediate-early (IE) gene expression. However, using a Ore reporter mouse system in
Arthur, J. +6 more
core +1 more source