Results 291 to 300 of about 98,107 (317)
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Conditional Gene Knockout Using Cre Recombinase
Molecular Biotechnology, 2001Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre's ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site.
Y, Le, B, Sauer
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Expression of Cre Recombinase in Pigment Cells
Pigment Cell Research, 2002Conditional gene targeting using the Cre/loxP system enables specific deletion of a gene in a tissue of interest. For application of Cre‐mediated recombination in pigment cells, Cre expression has to be targeted to pigment cells in transgenic mice. So far, no pigment cell‐specific Cre transgenic line has been reported and we present and discuss our ...
Laurence, Guyonneau +4 more
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Intein-Mediated Rapid Purification of Cre Recombinase
Protein Expression and Purification, 2001Cre recombinase produced by bacteriophage P1 catalyzes site-specific recombination of DNA between loxP recognition sites in both prokaryotic and eukaryotic cells and has been widely used for genome engineering and in vitro cloning. Recombinant Cre has been overproduced in Escherichia coli and its purification involves multiple steps. In this report, we
E J, Cantor, S, Chong
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Cre recombinase expression in cerebellar Purkinje cells
genesis, 2000The cerebellar cortex and its sole output, the Purkinje cell, have been implicated in motor coordination, learning and cognitive functions. Therefore, the ability to generate Purkinje cell-specific mutations in physiologically relevant genes is of particular neurobiological interest.
J J, Barski, K, Dethleffsen, M, Meyer
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Cre recombinase resources for conditional mouse mutagenesis
Methods, 2011Large scale international activities for systematic conditional mouse mutagenesis, exploiting advances in the sophisticated manipulation of the mouse genome, has established the mouse as the premier organism for developing models of human disease and drug action.
Damian Smedley +2 more
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α Complementation in the Cre recombinase enzyme
genesis, 2003AbstractSummary: The Cre‐loxP system is increasingly exploited for spatial and temporal gene inactivation. Here we present a novel approach to achieve this goal of selective gene inactivation. Following the model of α complementation in the β‐galactosidase enzyme, where the enzyme is split into independent polypeptides which are able to associate and ...
Emilio, Casanova +4 more
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Stem Cell Engineering Using Transducible Cre Recombinase
2007Embryonic stem (ES) cells have become a major focus of scientific interest both as a potential donor source for regenerative medicine and as a model system for tissue development and pathobiology. Tight and efficient methods for genetic engineering are required to exploit ES cells as disease models and to generate specific somatic phenotypes by lineage
Lars, Nolden +3 more
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Variegate expression of Cre recombinase in hematopoietic cells in CD11c-cre transgenic mice
Journal of Immunological Methods, 2023AbstractIn this study, we performed an in-depth analysis of Cre expression in the widely used CD11c-Cre transgenic mice generated by the group of Boris Reizis. In contrast to previous observation, using the highly sensitive Rosa-26-floxed-tdTomato reporter mouse line, we show variegated expression of Cre in multiple hematopoietic linage cells starting ...
Claire Murat, Sylvie Guerder
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Conditional Targeting: Inducible Deletion by Cre Recombinase
2007Tissue specific gene knockouts using Cre recombinase can have broad applicability in murine disease models of cardiovascular disease. The Cre system has been shown to have broad experimental versatility for both temporal and spatial control of gene deletion.
Kelly R, O'Neal, Ramtin, Agah
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Transgenic mice that express Cre recombinase in osteoclasts
genesis, 2004AbstractTo study the physiological control of osteoclasts, the bone resorbing cells, we generated transgenic mice carrying the Cre recombinase gene driven by either the tartrate‐resistant acid phosphatase (TRAP) or cathepsin K (Ctsk) promoters. TRAP‐Cre and Ctsk‐Cre transgenic mouse lines were characterized by breeding with LacZ ROSA 26 (R26R) reporter
Chiu, W. S. M. +5 more
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