Results 301 to 310 of about 98,107 (317)

Spontaneous recombinase activity of Cre–ERT2 in vivo

Transgenic Research, 2017
Inducible Cre-ERT recombinase technology is widely used for gene targeting studies. The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg(UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT.
Jasmin, Kristianto, Michael G, Johnson, Ryley K, Zastrow, Abigail B, Radcliff, Robert D, Blank   +4 more
openaire   +2 more sources

Novel recombination system using Cre recombinase alpha complementation

Biotechnology Letters, 2007
A major limitation for the use of Cre recombinase is its toxicity and a lack of temporal control over its activity. We have developed a new recombination system using Cre recombinase alpha-complementation. Cre recombinase was divided and one fragment (beta) was introduced into cells between two loxP sites with a CMV promoter in the upstream.
A. Seidi, M. Mie, EIRY KOBATAKE
openaire   +2 more sources

Induced DNA recombination by Cre recombinase protein transduction

genesis, 2002
AbstractSummary: Cre is a DNA recombinase that recognizes 34 base‐pair loxP sites of recombination. We have developed a cell‐permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP‐flanked targets by simply adding TATCre to cell cultures.
Sunil K, Joshi, Kahoko, Hashimoto, Pandelakis A, Koni   +2 more
openaire   +2 more sources

Cre recombinase‐mediated gene targeting of mesenchymal cells

genesis, 2004
AbstractLoss‐of‐function approaches by the Cre/loxP technology have provided powerful tools for functional analyses of genes of interest expressed preferentially in a particular tissue. Here we describe the generation of transgenic mouse lines expressing Cre recombinase under the control of the promoter/enhancer unit of the gene for the α2 chain of ...
Lore, Florin, Heike, Alter, Hermann-Josef, Gröne, Axel, Szabowski, Günther, Schütz, Peter, Angel   +5 more
openaire   +2 more sources

Thick ascending limb-specific expression of Cre recombinase

American Journal of Physiology-Renal Physiology, 2003
Evaluation of thick ascending limb (TAL) function has been hindered by the limited ability to selectively examine the function of this nephron segment in vivo. To address this, a Cre/loxP strategy was employed whereby the Tamm-Horsfall (THP) promoter was used to drive Cre recombinase expression in transgenic mice.
Peter K, Stricklett, Deborah, Taylor, Raoul D, Nelson, Donald E, Kohan   +3 more
openaire   +2 more sources

Red light responsive Cre recombinase for bacterial optogenetics

ACS Synthetic Biology
AbstractOptogenetic tools have been used in a wide range of microbial engineering applications that benefit from the tunable, spatiotemporal control that light affords. However, the majority of current optogenetic constructs for bacteria respond to blue light, limiting the potential for multichromatic control.
Fereshteh Jafarbeglou, Mary J. Dunlop
openaire   +2 more sources

Efficient, inducible Cre‐recombinase activation in vascular endothelium

genesis, 2008
AbstractIn recent years, gene‐targeting studies in mice have elucidated many molecular mechanisms in vascular biology. However, it has been difficult to apply this approach to the study of postnatal animals because mutations affecting the vasculature are often embryonically lethal.
Suzanne, Claxton, Vassiliki, Kostourou, Shalini, Jadeja, Pierre, Chambon, Kairbaan, Hodivala-Dilke, Marcus, Fruttiger   +5 more
openaire   +2 more sources

Functional expression of the Cre recombinase in actinomycetes

Applied Microbiology and Biotechnology, 2008
Site-specific recombinases revolutionized "in vivo" genetic engineering because they can catalyze precise excisions, integrations, inversions, or translocations of DNA between their distinct recognition target sites. We have constructed a synthetic gene encoding Cre recombinase with the GC content 67.7% optimized for expression in high-GC bacteria and ...
Fedoryshyn, Marta, Welle, E., Bechthold, Andreas, Luzhetskyy, Andriy   +3 more
openaire   +3 more sources

Preparing Mate-Paired Illumina Libraries Using Cre Recombinase

2017
Large insert mate pair reads have been used in de novo assembly and discovery of structural variants. We developed a new approach, Cre-LoxP inverse PCR paired end (CLIP-PE), which exploits the advantages of (1) Cre-LoxP recombination system to efficiently circularize large DNA fragments, (2) inverse PCR to enrich for the desired products that contain ...
Ze, Peng, Nandita, Nath, Zhiying, Zhao, Jeff L, Froula, Jan-Fang, Cheng, Feng, Chen   +5 more
openaire   +2 more sources

Engineering Cre Recombinase for Genome Engineering

2016
Cre recombinase recombines its DNA target, loxP sites, without help of accessory proteins or DNA repair systems. The simplicity of Cre-lox system has been widely utilized for genome editing, especially in mouse genetics. The goal of this dissertation is to constructCrerecombinase variants that will operate uponrecombination target sites (RTs) present ...
openaire   +2 more sources