Results 41 to 50 of about 98,107 (317)
Frontiers in Molecular Neuroscience, 2023 Improvements in the speed and cost of expression profiling of neuronal tissues offer an unprecedented opportunity to define ever finer subgroups of neurons for functional studies.
Manon Bohic +28 more
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Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells [PDF]
, 2012 The adaptive immune response to viral vectors reduces vector-mediated transgene expression from the brain. It is unknown, however, whether this loss is caused by functional downregulation of transgene expression or death of transduced cells.
Ahmed +60 more
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Generation of primary tumors with Flp recombinase in FRT-flanked p53 mice
Disease Models & Mechanisms, 2012 SUMMARY The site-specific recombinases Cre and Flp can mutate genes in a spatially and temporally restricted manner in mice. Conditional recombination of the tumor suppressor gene p53 using the Cre-loxP system has led to the development of multiple ...
Chang-Lung Lee +7 more
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Photoactivatable Cre recombinase 3.0 for in vivo mouse applications
Nature Communications, 2020 Previous versions of photoactivatable Cre recombinase (PA-Cre) suffered from unintentional recombination in dark conditions. Here, the authors develop an improved version of PA-Cre, called PA-Cre 3.0, which shows reduced leakiness and improved efficiency
Kumi Morikawa +16 more
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Synapsis of loxP Sites by Cre Recombinase [PDF]
Journal of Biological Chemistry, 2007 Cre recombinase catalyzes site-specific recombination between 34-bp loxP sites in a variety of topological and cellular contexts. An obligatory step in the recombination reaction is the association, or synapsis, of Cre-bound loxP sites to form a tetrameric protein assembly that is competent for strand exchange.
Kaushik, Ghosh +2 more
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Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending [PDF]
, 2016 Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicobacter
Adams +71 more
core +4 more sources
Molecular Brain, 2020 The defining features of a neuron are its functional and anatomical connections with thousands of other neurons in the brain. Together, these neurons form functional networks that direct animal behavior. Current approaches that allow the interrogation of
Audrey Tze Ting Khoo +3 more
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Endothelium‐specific Cre recombinase activity in flk‐1‐Cre transgenic mice [PDF]
Developmental Dynamics, 2004 AbstractThe use of the Cre‐loxP recombination system allows the conditional inactivation of genes in mice. The availability of transgenic mice in which the Cre recombinase expression is highly cell type specific is a prerequisite to successfully use this system.
Alexander H, Licht +3 more
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Mutants of Cre recombinase with improved accuracy [PDF]
, 2014 Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted towards use as transgene delivery ...
Church, George M., Eroshenko, Nikolai
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Cre-loxP Reporter Mouse Reveals Stochastic Activity of the Foxp3 Promoter
Frontiers in Immunology, 2019 Mouse models that combine specific loxP-flanked gene sequences with Cre recombinase expressed from cell-regulated promoters have become important tools to investigate gene function.
Peter D. Bittner-Eddy +2 more
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