Results 51 to 60 of about 98,107 (317)

Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation. [PDF]

PLoS Biology, 2014
Mechanisms behind how the immune system signals to the brain in response to systemic inflammation are not fully understood. Transgenic mice expressing Cre recombinase specifically in the hematopoietic lineage in a Cre reporter background display ...
Kirsten Ridder, Sascha Keller, Maria Dams, Anne-Kathleen Rupp, Jessica Schlaudraff, Domenico Del Turco, Julia Starmann, Jadranka Macas, Darja Karpova, Kavi Devraj, Candan Depboylu, Britta Landfried, Bernd Arnold, Karl H Plate, Günter Höglinger, Holger Sültmann, Peter Altevogt, Stefan Momma   +17 more
doaj   +1 more source

Viral Cre-LoxP tools aid genome engineering in mammalian cells

Journal of Biological Engineering, 2017
Background Targeted nucleases have transformed genome editing technology, providing more efficient methods to make targeted changes in mammalian genome.
Ranjita Sengupta, Amy Mendenhall, Nandita Sarkar, Chandreyee Mukherjee, Amirali Afshari, Joseph Huang, Biao Lu   +6 more
doaj   +1 more source

A Novel Cre/lox71-Based System for Inducible Expression of Recombinant Proteins and Genome Editing

Cells, 2022
In this study, we developed a novel Cre/lox71-based system for the controlled transient expression of target genes. We used the bacteriophage P1 Cre recombinase, which harbors a short, highly specific DNA-binding site and does not have endogenous binding
Maxim Karagyaur, Daniyar Dyikanov, Pyotr Tyurin-Kuzmin, Stalik Dzhauari, Mariya Skryabina, Maksim Vigovskiy, Alexandra Primak, Natalia Kalinina, Vsevolod Tkachuk   +8 more
doaj   +1 more source

Cre Recombinase

Microbiology Spectrum, 2015
ABSTRACT The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread. In addition to the numerous in vivo and in vitro applications that have been reported since Cre was first shown to function in ...
openaire   +2 more sources

A Transgenic Rat for Investigating the Anatomy and Function of Corticotrophin Releasing Factor Circuits. [PDF]

, 2015
Corticotrophin-releasing factor (CRF) is a 41 amino acid neuropeptide that coordinates adaptive responses to stress. CRF projections from neurons in the central nucleus of the amygdala (CeA) to the brainstem are of particular interest for their role in ...
Blasio, Angelo, Crawford, Elena, Dadgar, Jahan, De Guglielmo, Giordano, George, Olivier, Hopf, F Woodward, Janak, Patricia H, Keiflin, Ronald, Kharazia, Viktor, Maiya, Rajani, Messing, Robert O, Millan, E Zayra, Pomrenze, Matthew B, Rice, Kenner C   +13 more
core   +2 more sources

Functional study of residual iCre activity relevant for split-Cre applications

Current Research in Biotechnology
Cre-lox system is a major tool in mouse molecular genetics instrumental in promoting somatic recombination to spatiotemporally control transcriptional activation/inhibition in subsets of cells or tissues in vivo.
Antea Minetti, Noemi Barsotti, Massimo Pasqualetti   +2 more
doaj   +1 more source

A CreER Mouse to Study Melanin Concentrating Hormone Signaling in the Developing Brain [PDF]

, 2018
The neuropeptide, melanin concentrating hormone (MCH), and its G protein‐coupled receptor, melanin concentrating hormone receptor 1 (Mchr1), are expressed centrally in adult rodents. MCH signaling has been implicated in diverse behaviors such as feeding,
Antonellis, Patrick J., Bansal, Ruchi, Berbari, Nicolas F., Emond, Michelle R., Engle, Staci E., Jontes, James D., Kesterson, Robert A., Mykytyn, Kirk, Whitehouse, Logan S.   +8 more
core   +2 more sources

TNF receptor–related factor 3 inactivation promotes the development of intrahepatic cholangiocarcinoma through NF‐κB‐inducing kinase–mediated hepatocyte transdifferentiation

Hepatology, EarlyView., 2022
Abstract Background and Aims Intrahepatic cholangiocarcinoma (ICC) is a deadly but poorly understood disease, and its treatment options are very limited. The aim of this study was to identify the molecular drivers of ICC and search for therapeutic targets.
Yuto Shiode, Takahiro Kodama, Satoshi Shigeno, Kazuhiro Murai, Satoshi Tanaka, Justin Y. Newberg, Jumpei Kondo, Shogo Kobayashi, Ryoko Yamada, Hayato Hikita, Ryotaro Sakamori, Hiroshi Suemizu, Tomohide Tatsumi, Hidetoshi Eguchi, Nancy A. Jenkins, Neal G. Copeland, Tetsuo Takehara   +16 more
wiley   +1 more source

Myh6-driven Cre recombinase activates the DNA damage response and the cell cycle in the myocardium in the absence of loxP sites

Disease Models & Mechanisms, 2020
Regeneration of muscle in the damaged myocardium is a major objective of cardiovascular research, for which purpose many investigators utilize mice containing transgenes encoding Cre recombinase to recombine loxP-flanked target genes. An unfortunate side
Xinrui Wang, Amelia Lauth, Tina C. Wan, John W. Lough, John A. Auchampach   +4 more
doaj   +1 more source

Specificity and off-target effects of AAV8-TBG viral vectors for the manipulation of hepatocellular gene expression in mice

Biology Open, 2021
Mice are a widely used pre-clinical model system in large part due to their potential for genetic manipulation. The ability to manipulate gene expression in specific cells under temporal control is a powerful experimental tool.
Christos Kiourtis, Ania Wilczynska, Colin Nixon, William Clark, Stephanie May, Thomas G. Bird   +5 more
doaj   +1 more source