Efficient CPP-mediated Cre protein delivery to developing and adult CNS tissues
BMC Biotechnology, 2009 Background Understanding and manipulating gene function in physiological conditions is a major objective for both fundamental and applied research. In contrast to other experimental settings, which use either purely genetic or gene delivery (viral or non-
Levi Giovanni +4 more
doaj +1 more source
Cre Recombinase: a genomic manipulation tool
The FASEB Journal, 2020 Comprehending human biology necessitates an understanding of molecular mechanisms that contribute to human physiology. For example, to determine why drug users relapse after stress, researchers must identify the neurological pathways involved in the addiction, and understand how certain stimuli trigger relapse.
David Richard Sampe +9 more
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Contribution of recent transgenic models and transcriptional profiling studies to our understanding of the mechanisms by which androgens control spermatogenesis [PDF]
, 2008 Publisher ...
Claessens, Frank +7 more
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Molecular Oncology, EarlyView.This work identified serum proteins associated with pancreatic epithelial neoplasms (PanINs) and early‐stage PDAC. Proteomics screens assessed genetically engineered mice with abundant PanINs, KPC mice (Lox‐STOP‐Lox‐KrasG12D/+ Lox‐STOP‐Lox‐Trp53R172H/+ Pdx1‐Cre) before PDAC development and also early‐stage PDAC patients (n = 31), compared to benign ...
Hannah Mearns +10 more
wiley +1 more source
Binary recombinase systems for high-resolution conditional mutagenesis [PDF]
, 2017 Conditional mutagenesis using Cre recombinase expressed from tissue specific promoters facilitates analyses of gene function and cell lineage tracing. Here, we describe two novel dual-promoter-driven conditional mutagenesis systems designed for greater ...
Hermann, Mario +9 more
core
Myocardial deletion of Smad4 using a novel alpha skeletal muscle actin Cre recombinase transgenic mouse causes misalignment of the cardiac outflow tract [PDF]
, 2010 S MAD4 acts as the converging point f or T GF β and BMP signaling in heart development. Here, we investigated the role of S MAD4 in heart development usi ng a novel α skeletal muscle actin Cre recombinase (MuCre) transgenic mouse strain.
Azhar, M +6 more
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Visual Recovery Reflects Cortical MeCP2 Sensitivity in Rett Syndrome
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Rett syndrome (RTT) is a devastating neurodevelopmental disorder with developmental regression affecting motor, sensory, and cognitive functions. Sensory disruptions contribute to the complex behavioral and cognitive difficulties and represent an important target for therapeutic interventions.
Alex Joseph Simon +12 more
wiley +1 more source
Detection and organ-specific ablation of neuroendocrine cells by synaptophysin locus-based BAC cassette in transgenic mice. [PDF]
PLoS ONE, 2013 The role of cells of the diffuse neuroendocrine system in development and maintenance of individual organs and tissues remains poorly understood. Here we identify a regulatory region sufficient for accurate in vivo expression of synaptophysin (SYP), a ...
Chieh-Yang Cheng +2 more
doaj +1 more source
Analysis of AtCLV3 promoter and its utilization for expressing cre recombinase in Indian mustard
The Indian Journal of Agricultural Sciences, 2021 Constitutive expression of the transgene is not always desirable because of its pleiotropic effects and metabolic pay-off. Therefore, tissue-specific expression of the transgene can be more significant in reducing the unintended effects of the transgene.
DEEPA DHATWALIA +4 more
doaj +1 more source
SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. [PDF]
, 2013 The secretory pathway of eukaryotic cells packages cargo proteins into COPII-coated vesicles for transport from the endoplasmic reticulum (ER) to the Golgi.
Adams, Elizabeth +17 more
core +1 more source