Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
Digital literacy and creative teaching behavior among nursing educators: the chain mediating roles of self-efficacy and digital stress. [PDF]
Chong-Wen W +5 more
europepmc +1 more source
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
How Creative Self-Regulation Is Associated with Adolescent Creativity: The Sequential Mediating Roles of Cognitive Flexibility and Creative Self-Efficacy. [PDF]
Xu X, Tang S, Chen T, Jin H.
europepmc +1 more source
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Editorial: At the borders of movement, art, and neurosciences, volume II. [PDF]
Cheron G +4 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
How instructional leadership and creativity related factors shape students' reading performance: evidence from a Bayesian multilevel mediation approach using PISA 2022 Türkiye data. [PDF]
Aydemir NE, Fidan T.
europepmc +1 more source
Cognitive underpinnings of malevolent creativity: the role of problem construction. [PDF]
Linnell A, Reiter-Palmon R.
europepmc +1 more source
Children's and adolescents' perceptions of Discovery Colleges in Germany: low awareness but high acceptability. [PDF]
Omundo J +5 more
europepmc +1 more source

