Results 301 to 310 of about 1,254,871 (374)

Characterization and engineering of a type IV-A3 CRISPR-Cas system for genome editing in Escherichia coli. [PDF]

Nucleic Acids Res
Semsey S, Søndberg E, Røen M, Hallström B, Petersen AØ, Alfastsen L, Bosch BR, Wohl B, Clube J, van der Helm E, Groendahl C, Mougiakos I.   +11 more
europepmc   +1 more source

Correction: DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination

Ruijie Deng, Yaxuan Bai, Yumei Liu, Yunhao Lu, Zhifeng Zhao, Yi Deng, Hao Yang   +6 more
openalex   +2 more sources

SETDB2 Mitigates Podocyte Dysfunction in Diabetic Kidney Disease Through Epigenetic Silencing of SMAD3

Advanced Science, EarlyView.
SETDB2 epigenetically represses Smad3 transcription by increasing H3K9me3 enrichment at its promoter, thereby mitigating podocyte dysfunction in DKD. The transcription factor TCF21 binds directly to the Setdb2 promoter and enhances its expression in podocytes. Abstract Podocyte dysfunction represents both an early pathological hallmark and a key driver
Lanfang Li, Shimin Jiang, Qi Jin, Peng Qu, Yingjie Guo, Xushan Lan, Xinyu Li, Cuiting Sun, Sinan Ai, Xin Li, Weiliang Sun, Jing Guo, Wenge Li, Liang Peng, Lihong Liu   +14 more
wiley   +1 more source

CRISPR-Cas-Based Diagnostics in Biomedicine: Principles, Applications, and Future Trajectories. [PDF]

Biosensors (Basel)
Zhou Z, Cho IH, Kadam US.
europepmc   +1 more source

Targeted Extracellular Vesicles Deliver Asiaticoside to Inhibit AURKB/DRP1‐Mediated Mitochondrial Fission and Attenuate Hypertrophic Scar Formation

Advanced Science, EarlyView.
Hypertrophic scar formation is driven by excessive mitochondrial fission in wound macrophages, which we discover is governed by a novel AURKB‐DRP1(Ser616) axis. The study develops a targeted therapy using cRGD‐decorated extracellular vesicles to deliver the natural compound Asiaticoside specifically to macrophages.
Luyu Li, Chenli Si, Xue Wang, Xiaojin Wu, Ying Shang, Shengfang Ge, Yong Wang, Yong Zuo, Zhen Zhang   +8 more
wiley   +1 more source

CARF-HAD phosphatase effectors provide immunity during the type III-A CRISPR-Cas response. [PDF]

Nucleic Acids Res
Stella G, Ye L, Brady SF, Marraffini L.
europepmc   +1 more source

GPCRs in CAR‐T Cell Immunotherapy: Expanding the Target Landscape and Enhancing Therapeutic Efficacy

Advanced Science, EarlyView.
Chimeric antigen receptor T cell therapy faces dual challenges of target scarcity and an immunosuppressive microenvironment in solid tumors. This review highlights how G protein‐coupled receptors can serve as both novel targets to expand the therapeutic scope and functional modules to enhance CAR‐T cell efficacy.
Zhuoqun Liu, Yuchen Xiao, Yamin Zhao, Lihe Dai, David J.H. Shih, Shikang Liang, Honglei Tian, Liu Liu, Feng Tian, Bing Liu, Lei Chang, Chao Zhang   +11 more
wiley   +1 more source

A Modular and Customizable CRISPR/Cas Toolkit for Epigenome Editing of Cis-regulatory Modules. [PDF]

Adv Sci (Weinh)
Zhang L, Fu J, Long T, Zhang C, Fan F, Lang Z, Zhu JK.   +6 more
europepmc   +1 more source

EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis

Advanced Science, EarlyView.
The translation factor Eukaryotic translation initiation factor 5A (eIF5A) is essential for male fertility in mice. It supports the translation of proteins crucial for heterochromatin organization and acrosome formation. eIF5A deficiency disrupts chromocenter integrity, increases chromatin accessibility, and causes transcriptional dysregulation ...
Yuling Cai, Tongtong Li, Qian Fang, Ziyou Bao, Feng Kong, Huitao Qi, Hanzhen Li, Mingyu Zhang, Wei Wang, Yongxia Guan, Wenbo Liu, Xiangfeng Chen, Zi‐Jiang Chen, Xiaohua Jiang, Xin Wang, Hongbin Liu   +15 more
wiley   +1 more source