Results 311 to 320 of about 1,254,871 (374)

Design of highly functional genome editors by modelling CRISPR-Cas sequences. [PDF]

Nature
Ruffolo JA, Nayfach S, Gallagher J, Bhatnagar A, Beazer J, Hussain R, Russ J, Yip J, Hill E, Pacesa M, Meeske AJ, Cameron P, Madani A.   +12 more
europepmc   +1 more source

Antiviral CRISPR-Cas strategies

Combination antiretroviral therapy (cART) can effectively inhibit replication of the human immunodeficiency virus (HIV) and prevent progression to AIDS. However none of the currently available drugs inactivate the HIV DNA that is integrated on one of the chromosomes of all infected cells.
openaire   +1 more source

Integrin β3 Orchestrates Hepatic Steatosis via a Novel CD36‐Dependent Lipid Uptake Complex

Advanced Science, EarlyView.
In MASH, ITGB3 recruits LYN and drives its ubiquitin‐proteasomal degradation via phosphorylation. This relieves DHHC5 inhibition, enabling ITGB3/DHHC5/CD36 complex assembly to enhance CD36 palmitoylation and fatty acid uptake, thereby exacerbating disease. Targeting ITGB3 blocks this pathogenic axis and ameliorates MASH.
Ying Zhang, Lei Dai, Zhongquan Cui, Ye Zhou, Wenyuan Dong, Hongcheng Jiang, Mengwen Wang, Xiaodan Zhong, Wei Dong, Zhang Yue, Thati Madhusudhan, Hongjie Wang, Xiong Zhong Ruan, Hesong Zeng   +13 more
wiley   +1 more source

CRISPR-Cas targeting in <i>Haloferax volcanii</i> promotes within-species gene exchange by triggering homologous recombination. [PDF]

Microlife
Choudhary DK, Turgeman-Grott I, Robinzon S, Gophna U.   +3 more
europepmc   +1 more source

Droplet Digital CRISPR for Nucleic Acid Detection

Advanced Science, EarlyView.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang, Roy S. K. Walker, Anwar Sunna, Tracie J. Barber, Ming Li   +4 more
wiley   +1 more source

CRISPR-Cas in actinomycetes: still a lot to be discovered. [PDF]

Microlife
Mitousis L, Musiol-Kroll E, Wohlleben W.
europepmc   +1 more source

Genome‐Wide CRISPR Screen Reveals PIK3CA Inhibition Enhances Lipid Nanoparticle‐Mediated siRNA Delivery

Advanced Science, EarlyView.
A genome‐wide CRISPR screen and identifies PIK3CA as a key regulator of lipid nanoparticle (LNP) uptake is performed. Modulating PIK3CA with small‐molecule inhibitor BAY1082439 enhanced LNP uptake, siRNA delivery, and gene silencing in a variety of epithelial cells, and improved therapeutic outcomes in vivo.
Wenhan Wang, Kangfu Chen, Zongjie Wang
wiley   +1 more source

Exploring CRISPR-Cas: The transformative impact of gene editing in molecular biology. [PDF]

Mol Ther Nucleic Acids
Pandey V, Sharma S, Pokharel YR.
europepmc   +1 more source

Comparative Single‐Cell Transcriptomic Atlas Reveals the Genetic Regulation of Reproductive Traits

Advanced Science, EarlyView.
A cross‐species single‐cell transcriptomic atlas of reproductive and central nervous system tissues from sheep and humans reveals conserved cellular programs and regulatory networks that regulated fertility. Integration with GWAS for sheep lifetime average litter size identifies UNC5–SLIT–BMP signaling as a core pathway coordinating neuroendocrine ...
Bingru Zhao, Hanpeng Luo, Dailu Guan, Xuefeng Fu, Feng Wang, Guoming Zhang, Shanglai Li, Hua Yang, Yanli Zhang   +8 more
wiley   +1 more source

Cascade CRISPR/cas Enables More Sensitive Detection of <i>Toxoplasma gondii</i> and <i>Listeria monocytogenes</i> than Single CRISPR/cas. [PDF]

Microorganisms
Chen D, Sun M, Li B, Ma J, Zhang Q, Yin W, Li J, Wei M, Liu L, Yang P, Shen Y.   +10 more
europepmc   +1 more source