Results 341 to 350 of about 1,254,871 (374)

CRISPR/Cas‐Based MicroRNA Biosensors

Chemistry – A European Journal, 2023
AbstractAs important post‐transcriptional regulators, microRNAs (miRNAs) play irreplaceable roles in diverse cellular functions. Dysregulated miRNA expression is implicated in various diseases including cancers, and thus miRNAs have become the valuable biomarkers for disease monitoring.
Qian Zhang, Xinyi Zhang, Xiaoran Zou, Fei Ma, Chun‐Yang Zhang   +4 more
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RNA-targeting CRISPR–Cas systems

Nature Reviews Microbiology, 2022
CRISPR-Cas is a widespread adaptive immune system in bacteria and archaea that protects against viral infection by targeting specific invading nucleic acid sequences. Whereas some CRISPR-Cas systems sense and cleave viral DNA, type III and type VI CRISPR-Cas systems sense RNA that results from viral transcription and perhaps invasion by RNA viruses ...
Sam P. B. van Beljouw, Jasper Sanders, Alicia Rodríguez-Molina, Stan J. J. Brouns   +3 more
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Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives.

Chemical Society Reviews, 2023
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food safety, and environmental monitoring. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)-based detection, as a cutting-edged
Huimin Li, Yi Xie, Fumin Chen, Huiwen Bai, Leshan Xiu, Xiaoping Zhou, Xiaokui Guo, Qinqin Hu, K. Yin   +8 more
semanticscholar   +1 more source

CRISPR/Cas gene therapy

Journal of Cellular Physiology, 2020
AbstractClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated enzyme (Cas) is a naturally occurring genome editing tool adopted from the prokaryotic adaptive immune defense system. Currently, CRISPR/Cas9‐based genome editing has been becoming one of the most promising tools for treating human genetic diseases, including ...
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MicroRNA Detection with CRISPR/Cas

2023
Low-cost detection of miRNAs has caught broad attention in recent years due to the potential application of these small noncoding RNAs for diagnostics and therapeutic purposes. Their small size and low abundance, however, derive challenges in engineering robust detection tools.
Xinyuan, Qiu, Chuanyang, Liu, Chushu, Zhu, Lingyun, Zhu   +3 more
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CRISPR/Cas‐Powered Biosensing

Analysis & Sensing, 2022
AbstractCRISPR/Cas system is to be a powerful tool to construct the nucleic acid diagnostic platform. Specific recognition and cleavage of CRISPR/Cas systems enable selective recognition of analytes and physicochemical signal translation of biorecognition events.
Mengying Deng, Fengqin Li, Xiaolei Zuo, Min Li   +3 more
openaire   +1 more source

CRISPR-Cas immunity in prokaryotes

Nature, 2015
Prokaryotic organisms are threatened by a large array of viruses and have developed numerous defence strategies. Among these, only clustered, regularly interspaced short palindromic repeat (CRISPR)-Cas systems provide adaptive immunity against foreign elements. Upon viral injection, a small sequence of the viral genome, known as a spacer, is integrated
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CRISPR/Cas Powered Multiplexed Biosensing

Trends in Biotechnology, 2019
Multiplexed CRISPR/Cas biosensing offers various possibilities for diagnosing different diseases by analyzing many analytes from one single specimen. However, in this relatively new field, nearly no multiplexing approaches exist, as many challenges need to be addressed.
Bruch, Richard, Urban, Gerald A., Dincer, Can   +2 more
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CRISPR–Cas impedes archaeal mating

Nature Microbiology, 2018
The early identification of repetitive genomic loci in Haloferax species was instrumental in igniting interest in CRISPR–Cas systems. Now, work in this genus has revealed an important role of CRISPR–Cas in reducing an unusual form of inter-species archaeal mating that occurs by cell fusion.
openaire   +2 more sources