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Nature Cell Biology, 2019
MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina, Jouravleva, Phillip D, Zamore
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MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina, Jouravleva, Phillip D, Zamore
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ACM SIGGRAPH 2018 Posters, 2018
CRISPR/Cas9-NHEJ: Action in the Nucleus (2017) is derived from an interdisciplinary creative process. This paper discusses the creation of this 210° scientific visualization, the usage of data from the worldwide Protein Data Bank, and the audio-visual presentation in an interactive dome setup.
Martina R. Fröschl, Alfred Vendl
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CRISPR/Cas9-NHEJ: Action in the Nucleus (2017) is derived from an interdisciplinary creative process. This paper discusses the creation of this 210° scientific visualization, the usage of data from the worldwide Protein Data Bank, and the audio-visual presentation in an interactive dome setup.
Martina R. Fröschl, Alfred Vendl
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Nature Reviews Molecular Cell Biology, 2020
The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
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The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
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2018
In the present chapter, we present the protocols and guidelines to facilitate implementation of CRISPR-Cas9 technology in fungi where few or no genetic tools are in place. Hence, we firstly explain how to identify dominant markers for genetic transformation.
Jakob B, Hoof +2 more
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In the present chapter, we present the protocols and guidelines to facilitate implementation of CRISPR-Cas9 technology in fungi where few or no genetic tools are in place. Hence, we firstly explain how to identify dominant markers for genetic transformation.
Jakob B, Hoof +2 more
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CRISPR–Cas9 can cause chromothripsis
Nature Genetics, 2021Genome editing with CRISPR–Cas9 is beginning to be used clinically; promising results to date inspire hope for broad medical impact and mindfulness about safety. A new study shows that when Cas9 cuts its target, a fraction of the time, the target chromosome experiences a breakage process known as chromothripsis, thus prompting efforts to understand the
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2023
Students can use this worksheet to collect information regarding the process of CRISPR ...
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Students can use this worksheet to collect information regarding the process of CRISPR ...
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Liposomal delivery of CRISPR/Cas9
Cancer Gene Therapy, 2019Liposomes are one of the most widely investigated carriers for CRISPR/Cas9 delivery. The surface properties of liposomal carriers, including the surface charge, PEGylation, and ligand modification can significantly affect the gene silencing efficiency. Three barriers of systemic CRISPR/Cas9 delivery (long blood circulation, efficient tumor penetration,
Shuai, Zhen, Xu, Li
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Cascading CRISPR–Cas9 genome edits
Nature Reviews Genetics, 2021In this study in Molecular Cell, Clarke et al. describe a system that enables multiple Cas9-mediated genome edits to be introduced into cells in a defined, sequential order.
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CRISPR–Cas9 Structures and Mechanisms
Annual Review of Biophysics, 2017Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA.
Fuguo, Jiang, Jennifer A, Doudna
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