Results 101 to 110 of about 58,663 (324)

Identifying prognostic targets in metastatic prostate cancer beyond AR

FEBS Open Bio, EarlyView.
Genome‐wide functional screens combined with a large gene expression database and clinical outcomes can identify new therapeutic vulnerabilities in prostate cancer. Eight potentially druggable targets demonstrated strong dependency in cell lines, were associated with worse prognosis clinically, and showed evidence of protein expression in prostate ...
Emily Feng, Eric Feng, Tracy Berg, Isabella S. Nguyen, Lilac G. Nguyen, William Chen, Meng Zhang, David Quigley, Marina Sharifi, Haolong Li, Ilsa Coleman, Peter S. Nelson, Martin Sjöström, Shuang G. Zhao   +13 more
wiley   +1 more source

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. [PDF]

, 2018
CRISPR-Cas systems provide microbes with adaptive immunity to infectious nucleic acids and are widely employed as genome editing tools. These tools use RNA-guided Cas proteins whose large size (950 to 1400 amino acids) has been considered essential to ...
Banfield, JF, Burstein, D, Chen, JS, Cofsky, JC, Doudna, JA, Harrington, LB, Kyrpides, NC, Ma, E, Paez-Espino, D, Witte, IP   +9 more
core  

CRISPR-Cas systems target endogenous genes to impact bacterial physiology and alter mammalian immune responses

Molecular Biomedicine, 2022
CRISPR-Cas systems are an immune defense mechanism that is widespread in archaea and bacteria against invasive phages or foreign genetic elements.
Qun Wu, Luqing Cui, Yingying Liu, Rongpeng Li, Menghong Dai, Zhenwei Xia, Min Wu   +6 more
doaj   +1 more source

CRISPR-Cas Systems and Genome Editing: Beginning the Era of CRISPR/Cas Therapies for Humans. [PDF]

Int J Mol Sci
Harnessing of CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated genes) systems for detection, chemical modification, and sequence editing of nucleic acids dramatically changed many fields of fundamental science, biotechnology, and biomedicine [...]
Karpov DS.
europepmc   +3 more sources

CNS Mitochondria‐Derived Vesicle in Blood: Potential Biomarkers for Brain Mitochondria Dysfunction

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial dysfunction is a hallmark of neurodegenerative diseases like Alzheimer's (AD) and Parkinson's (PD). Our goal was to develop practical, noninvasive methods to assess mitochondrial status through the detection of mitochondria‐derived vesicles (MDVs).
Qi Liu, Wentao Chen, Yahong Wu, Zhen Guo, Jun Chen, Chen Tian, Pan Wang, Shaopeng Zeng, Bin Xu, Jing Duan, Shilong Han, Xiao Xiong, Jing Zhang   +12 more
wiley   +1 more source

Development and Application of CRISPR/Cas in Microbial Biotechnology

Frontiers in Bioengineering and Biotechnology, 2020
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods ...
Wentao Ding, Wentao Ding, Yang Zhang, Shuobo Shi   +3 more
doaj   +1 more source

The Regulation of Trace Metal Elements in Cancer Ferroptosis

Advanced Biology, EarlyView.
The induction of ferroptosis inhibits tumor growth, enhances anticancer efficacy, and overcomes drug resistance. Recent evidence shows nonferrous metal elements play a role in ferroptosis. This review focuses on how trace metals regulate ferroptosis processes like iron accumulation, lipid peroxidation, and antioxidant defense.
Xiaoyan Wang, Yuanyuan Xue, Lei Chang, Xuena Zhu, Wenjun Liu, Tingbo Liang   +5 more
wiley   +1 more source

CRISPR-Cas in Escherichia coli: regulation by H-NS, LeuO and temperature [PDF]

, 2020
CRISPR-Cas adaptive immune systems are present in many bacteria and archaea and provide protection against invading DNA such as phages and plasmids. These systems are very versatile and complex in their gene composition and genomic architecture.
Damjan Mitić, Dora Markulin, Ivana Ivančić Baće, Marin Radovčić   +3 more
core   +2 more sources

Alleviation of Aging‐Related Hallmarks in a Mouse Model of Progeria via a Nanoparticle‐Based Artificial Transcription Factor

Advanced Functional Materials, EarlyView.
Oct4‐nanoscript, a biomimetic nanoparticle‐based artificial transcription factor, precisely regulates cellular rejuvenation by activating Oct4 target genes, restoring epigenetic marks, and reducing DNA damage. In a progeria model, it effectively rescued aging‐associated pathologies and extended lifespan.
Hongwon Kim, Junyeop Kim, Euiyeon Lee, Brandon Conklin, Yannan Hou, Sumin Kim, Yerim Hwang, Ki‐Bum Lee, Jongpil Kim   +8 more
wiley   +1 more source

Synthetic Strategy for mRNA Encapsulation and Gene Delivery with Nanoscale Metal‐Organic Frameworks

Advanced Functional Materials, EarlyView.
This research utilizes the ZIF‐8 for the encapsulation and intracellular delivery of nucleic acids, specifically mRNA, for applications in gene delivery. Integrating PEI addresses the issue of mRNA leakage from ZIF‐8, resulting in the delivery and expression of green fluorescent protein (GFP) in vitro and firefly luciferase in vivo.
Harrison Douglas Lawson, Huy Hoang Nguyen, Keng‐Jung Lee, Nattarat Wongsuwan, Ayesha Tupe, Mengrou Lu, Mariah Lynn Arral, Anne Behre, Zihan Ling, Kathryn Ann Whitehead, Adam Walter Feinberg, Xi Ren, Si‐Yang Zheng   +12 more
wiley   +1 more source