Results 101 to 110 of about 64,886 (293)

Advances in biotechnology: genomics and genome editing [PDF]

, 2017
Genomics, the study of genes, their functions and related techniques has become a crucial science for developing understanding of life processes and how they evolve.
Beccari, Tommaso, Dundar, Munis, Gartland, Jill S., Gartland, Kevan M.A., Magni, Mariapia Viola   +4 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Development and Application of CRISPR/Cas System in Rice

Rice Science, 2019
In the past several years, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system has been harnessed as an efficient and powerful tool for targeted genome editing in different prokaryotic and ...
Ren Jun, Hu Xixun, Wang Kejian, Wang Chun   +3 more
doaj   +1 more source

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

BIOINFORMATIC SEARCH OF CRISPR/CAS SYSTEM STRUCTURES IN GENOME OF PCT281 PLASMID OF BACILLUS THURINGIENSIS SUBSP. CHINENSIS STRAIN CT-43

Acta Biomedica Scientifica, 2018
Background. CRISPR/Cas systems loci are one of the functionally important patterns in bacterial genome which perform the role of “adaptive immune defense” from foreign nucleic acids.
N. A. Arefyeva, Yu. P. Dzhioev, A. Yu. Borisenko, V. I. Chemerilova, O. F. Vyatchina, O. A. Sekerina, L. A. Stepanenko, Yu. A. Markova, G. V. Yurinova, V. P. Salovarova, A. A. Pristavka, V. A. Kuzminova, O. N. Reva, V. I. Zlobin   +13 more
doaj   +1 more source

Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. [PDF]

, 2018
The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus.
Chen, Yuan-Chuan, Liu, Fenyong, Sheng, Jingxue, Trang, Phong   +3 more
core   +2 more sources

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

The CRISPR-Cas system

, 2020
Derived from and inspired by the adaptive immune system of bacteria, CRISPR has gone from basic biology knowledge to a revolutionizing biotechnological tool, applicable in many research areas such as medicine, industry and agriculture. The full mechanism of CRISPR-Cas9 was first published in 2012 and various CRISPR-Cas systems have already passed the ...
Stens, Cassandra, Enoksson, Isabella, Berggren, Sara   +2 more
openaire   +1 more source

Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells. [PDF]

, 2019
Repurposed CRISPR-Cas molecules provide a useful tool set for broad applications of genomic editing and regulation of gene expression in prokaryotes and eukaryotes. Recent discovery of phage-derived proteins, anti-CRISPRs, which serve to abrogate natural
Abbott, Timothy R, Bondy-Denomy, Joseph, Carter, Matthew A, Chavez, Michael, Dominguez, Antonia A, Gao, Yuchen, Kipniss, Nathan H, La Russa, Marie, Lau, Matthew B, Nakamura, Muneaki, Qi, Lei S, Smolke, Christina D, Srinivasan, Prashanth, Xu, Xiaoshu, Zhao, Dehua   +14 more
core   +3 more sources