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CRISPR: An overview of CRISPR-Cas systems and their diagnostic methods for pathogen detection

Life Sciences, Medicine and Biomedicine
Early and rapid diagnosis of pathogens is crucial for preventing and controlling epidemic diseases. While traditional microbial detection methods serve their purpose, they often have limitations such as low sensitivity and specificity.
Pui Yuei Lee, Christina Shook Cheng Chong, Mee Hoong See, Lee Lee Lai, Paul Michels, Choon Jiat Lee, Rukumani Devi Velayuthan, Yoke Kiet Doon, Yong Hui Tan, Yin Yin Lau, Sze Wan Poong, Phaik Eem Lim, Crystale Siew Ying Lim   +12 more
doaj   +3 more sources

Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system [PDF]

Heliyon
CRISPR-Cas, the adaptive immune system exclusive to prokaryotes, confers resistance against foreign mobile genetic elements. The CRISPR-Cas system is now being exploited by scientists in a diverse range of genome editing applications.
Hui Cheng, Haoyue Deng, Dongdao Ma, Mengyuan Gao, Zhihan Zhou, Heng Li, Shejuan Liu, Tieshan Teng   +7 more
doaj   +2 more sources

Beyond adaptive immunity: Functional diversity of the type III-A CRISPR-Cas system in Mycobacterium tuberculosis [PDF]

Cell Insight
Wenjing Yu, Xindi Huang, Yangbo Hu, Shiyun Chen   +3 more
openalex   +2 more sources

AcrIIA7 hijacks tracrRNA to block CRISPR-Cas system [PDF]

Nat Commun
Hyun Ho Park, So Yeon Lee
europepmc   +3 more sources

[Research Progress on the Application of CRISPR/Cas System in Rapid Testing of Drug-Resistant Bacteria in Clinical Practice]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Zhen Song, Hui Li, Yanan Zhao, Min Li
openalex   +2 more sources

Analysis of genetic structure and function of clustered regularly interspaced short palindromic repeats loci in 110 Enterococcus strains

Frontiers in Microbiology, 2023
Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) are an adaptive immune system involved in specific defenses against the invasion of foreign mobile genetic elements, such as plasmids and phages.
Shuan Tao, Shuan Tao, Dongdong Zhou, Huimin Chen, Na Li, Lin Zheng, Yewei Fang, Yao Xu, Qi Jiang, Wei Liang   +9 more
doaj   +1 more source

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

Diagnostics, 2022
The CRISPR/Cas system is a protective adaptive immune system against attacks from foreign mobile genetic elements. Since the discovery of the excellent target-specific sequence recognition ability of the CRISPR/Cas system, the CRISPR/Cas system has shown
Junwei Li, Yuexia Wang, Bin Wang, Juan Lou, Peng Ni, Yuefei Jin, Shuaiyin Chen, Guangcai Duan, Rongguang Zhang   +8 more
doaj   +1 more source

An Endogenous Staphylococcus aureus CRISPR-Cas System Limits Phage Proliferation and Is Efficiently Excised from the Genome as Part of the SCCmec Cassette

Microbiology Spectrum, 2023
CRISPR-Cas is an adaptive immune system that allows bacteria to inactivate mobile genetic elements. Approximately 50% of bacteria harbor CRISPR-Cas; however, in the human pathogen Staphylococcus aureus, CRISPR-Cas loci are less common and often studied ...
Kasper Mikkelsen, Janine Zara Bowring, Yong Kai Ng, Frida Svanberg Frisinger, Julie Kjærsgaard Maglegaard, Qiuchun Li, Raphael N. Sieber, Andreas Petersen, Paal Skytt Andersen, Jakob T. Rostøl, Nina Molin Høyland-Kroghsbo, Hanne Ingmer   +11 more
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CRISPR-Cas in Acinetobacter baumannii Contributes to Antibiotic Susceptibility by Targeting Endogenous AbaI

Microbiology Spectrum, 2022
Acinetobacter baumannii is a well-known human opportunistic pathogen in nosocomial infections, and the emergence of multidrug-resistant Acinetobacter baumannii has become a complex problem for clinical anti-infective treatments.
Yuhang Wang, Jie Yang, Xiaoli Sun, Mengying Li, Pengyu Zhang, Zhongtian Zhu, Hongmei Jiao, Tingting Guo, Guocai Li   +8 more
doaj   +1 more source

Whole-Genome Analysis of Acinetobacter baumannii Strain AB43 Containing a Type I-Fb CRISPR-Cas System: Insights into the Relationship with Drug Resistance

Molecules, 2022
The CRISPR-Cas system is a bacterial and archaea adaptive immune system and is a newly recognized mechanism for controlling antibiotic resistance gene transfer. Acinetobacter baumannii (A.
Tingting Guo, Jie Yang, Xiaoli Sun, Yuhang Wang, Liying Yang, Guimei Kong, Hongmei Jiao, Guangyu Bao, Guocai Li   +8 more
doaj   +1 more source