Results 21 to 30 of about 45,956 (217)

Characterization of CRISPR-Cas Systems in Clinical Klebsiella pneumoniae Isolates Uncovers Its Potential Association With Antibiotic Susceptibility

Frontiers in Microbiology, 2018
Prokaryotic CRISPR-Cas systems limit the acquisition of genetic elements and provide immunity against invasive bacteriophage. The characteristics of CRISPR-Cas systems in clinical Klebsiella pneumoniae isolates are still unknown. Here, 97 K.
Hsin-Yu Li, Cheng-Yen Kao, Wei-Hung Lin, Po-Xing Zheng, Jing-Jou Yan, Ming-Cheng Wang, Ching-Hao Teng, Chin-Chung Tseng, Jiunn-Jong Wu   +8 more
doaj   +1 more source

Development and Application of CRISPR/Cas in Microbial Biotechnology

Frontiers in Bioengineering and Biotechnology, 2020
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods ...
Wentao Ding, Wentao Ding, Yang Zhang, Shuobo Shi   +3 more
doaj   +1 more source

CRISPR/Cas: A powerful tool for gene function study and crop improvement

Journal of Advanced Research, 2021
Background: It is a long-standing goal of scientists and breeders to precisely control a gene for studying its function as well as improving crop yield, quality, and tolerance to various environmental stresses.
Dangquan Zhang, Zhiyong Zhang, Turgay Unver, Baohong Zhang   +3 more
doaj   +1 more source

Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus

Frontiers in Microbiology, 2023
Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S.
Richard Bauer, Dorina Haider, Aline Grempels, Rebecca Roscher, Stefanie Mauerer, Barbara Spellerberg   +5 more
doaj   +1 more source

CRISPR/Cas systems: Delivery and application in gene therapy

Frontiers in Bioengineering and Biotechnology, 2022
The CRISPR/Cas systems in prokaryotes such as bacteria and archaea are the adaptive immune system to prevent infection from viruses, phages, or other foreign substances. When viruses or phages first invade the bacteria, Cas proteins recognize and cut the
Jie Huang, Yitong Zhou, Jie Li, Aiping Lu, Aiping Lu, Aiping Lu, Chao Liang, Chao Liang, Chao Liang   +8 more
doaj   +1 more source

CRISPR: An overview of CRISPR-Cas systems and their diagnostic methods for pathogen detection

Life Sciences, Medicine and Biomedicine
Early and rapid diagnosis of pathogens is crucial for preventing and controlling epidemic diseases. While traditional microbial detection methods serve their purpose, they often have limitations such as low sensitivity and specificity.
Pui Yuei Lee, Christina Shook Cheng Chong, Mee Hoong See, Lee Lee Lai, Paul Michels, Choon Jiat Lee, Rukumani Devi Velayuthan, Yoke Kiet Doon, Yong Hui Tan, Yin Yin Lau, Sze Wan Poong, Phaik Eem Lim, Crystale Siew Ying Lim   +12 more
doaj   +1 more source

Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant Pseudomonas aeruginosa

mBio, 2015
Pseudomonas aeruginosa is an antibiotic-refractory pathogen with a large genome and extensive genotypic diversity. Historically, P. aeruginosa has been a major model system for understanding the molecular mechanisms underlying type I clustered regularly ...
Alex van Belkum, Leah B. Soriaga, Matthew C. LaFave, Srividya Akella, Jean-Baptiste Veyrieras, E. Magda Barbu, Dee Shortridge, Bernadette Blanc, Gregory Hannum, Gilles Zambardi, Kristofer Miller, Mark C. Enright, Nathalie Mugnier, Daniel Brami, Stéphane Schicklin, Martina Felderman, Ariel S. Schwartz, Toby H. Richardson, Todd C. Peterson, Bolyn Hubby, Kyle C. Cady   +20 more
doaj   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Bioinformational analysis of Yersinia pseudotuberculosis IP32953 CRISPR/cas system

Acta Biomedica Scientifica, 2016
The results of this study include Yersinia pseudotuberculosis CRISPR/Cas system structure analysis. CRISPR/Cas system is a specific adaptive protection against heterogeneous genetic elements.
N. P. Peretolchina, Y. P. Dzhioev, A. Y. Borisenko, E. A. Voskresenskaya, A. I. Paramonov, L. A. Stepanenko, O. V. Kolbaseeva, V. I. Zlobin   +7 more
doaj   +1 more source

CRISPR-Cas System in Antibiotic Resistance Plasmids in Klebsiella pneumoniae

Frontiers in Microbiology, 2020
CRISPR-Cas (clustered regularly interspersed short palindromic repeats-CRISPR-associated protein) is a microbial adaptive immune system involved in defense against different types of mobile genetic elements.
Muhammad Kamruzzaman, Jonathan R. Iredell, Jonathan R. Iredell   +2 more
doaj   +1 more source