Results 41 to 50 of about 18,275,652 (330)

Cas1 and Cas2 From the Type II-C CRISPR-Cas System of Riemerella anatipestifer Are Required for Spacer Acquisition

Frontiers in Cellular and Infection Microbiology, 2018
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins provide acquired genetic immunity against the entry of mobile genetic elements (MGEs).
Yang He, Yang He, Yang He, Mingshu Wang, Mingshu Wang, Mingshu Wang, Mafeng Liu, Mafeng Liu, Mafeng Liu, Li Huang, Li Huang, Li Huang, Chaoyue Liu, Chaoyue Liu, Chaoyue Liu, Xin Zhang, Xin Zhang, Xin Zhang, Haibo Yi, Haibo Yi, Haibo Yi, Anchun Cheng, Anchun Cheng, Anchun Cheng, Dekang Zhu, Dekang Zhu, Qiao Yang, Qiao Yang, Qiao Yang, Ying Wu, Ying Wu, Ying Wu, Xinxin Zhao, Xinxin Zhao, Xinxin Zhao, Shun Chen, Shun Chen, Shun Chen, Renyong Jia, Renyong Jia, Renyong Jia, Shaqiu Zhang, Shaqiu Zhang, Shaqiu Zhang, Yunya Liu, Yunya Liu, Yunya Liu, Yanling Yu, Yanling Yu, Yanling Yu, Ling Zhang, Ling Zhang, Ling Zhang   +52 more
doaj   +1 more source

The virulence factor regulator and quorum sensing regulate the type I-F CRISPR-Cas mediated horizontal gene transfer in Pseudomonas aeruginosa

Frontiers in Microbiology, 2022
Pseudomonas aeruginosa is capable of thriving in diverse environments due to its network of regulatory components for effective response to stress factors.
Stephen Dela Ahator, Stephen Dela Ahator, Yang Liu, Yang Liu, Jianhe Wang, Lian-Hui Zhang   +5 more
doaj   +1 more source

DNA Nanomaterials for Delivery of Clustered Regularly Interspaced Short Palindromic Repeats/Cas Systems

Small Structures, 2023
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein (CRISPR/Cas) system has been exploited as an efficient gene editing tool with precise site specificity and high efficiency; the delivery of the CRISPR/Cas system is
Zhaoyue Lv, Zhemian Li, Peiran Li, Chi Yao, Dayong Yang   +4 more
doaj   +1 more source

CRISPR-Cas Systems Reduced to a Minimum [PDF]

Molecular Cell, 2019
In two recent studies in Molecular Cell, Wright et al. (2019) report complete spacer integration by a Cas1 mini-integrase and Edraki et al. (2019) describe accurate genome editing by a small Cas9 ortholog with less stringent PAM requirements.
Almendros, Cristóbal, Kieper, Sebastian N., Brouns, Stan J.J.   +2 more
openaire   +3 more sources

Determination of Acr-mediated immunosuppression in Pseudomonas aeruginosa

MethodsX, 2023
Bacteria have a broad array of defence mechanisms to fight bacteria-specific viruses (bacteriophages, phages) and other invading mobile genetic elements. Among those mechanisms, the ‘CRISPR-Cas’ (Clustered Regularly Interspaced Short Palindromic Repeats –
Benoit J. Pons, Edze R. Westra, Stineke van Houte   +2 more
doaj   +1 more source

Multiple nucleic acid cleavage modes in divergent type III CRISPR systems [PDF]

, 2016
CRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of transcription-dependent DNA targeting ...
Zhang, Jing, White, Malcolm Frederick, Tello, Agnes, Liu, Huanting, Graham, Shirley   +4 more
core   +1 more source

Development and Application of CRISPR/Cas in Microbial Biotechnology

Frontiers in Bioengineering and Biotechnology, 2020
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods ...
Wentao Ding, Wentao Ding, Yang Zhang, Shuobo Shi   +3 more
doaj   +1 more source

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition [PDF]

, 2015
This work was supported by a grant from the Biotechnology and Biological Sciences Research Council (REF: BB/M000400/1 to MFW).The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements.
Bolt, Edward, White, Malcolm F., Rollie, C, White, Malcolm F, Anna Sophie Brinkmann, Malcolm F White, Clare Rollie, Edward L Bolt, Brinkmann, Anna Sophie, Rollie, Clare, Bolt, EL, Rollie, Clare Jane Catherine, Schneider, Stefanie, White, MF, Stefanie Schneider, Bolt, Edward L., Schneider, S, Brinkmann, AS   +17 more
core   +1 more source

CRISPR-mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus [PDF]

, 2014
European SulfoSYS-project [SysMo P–N-01-09-23] and grant [9P23000 and P25369] by the Austrian Research fund (to C.S.) and by grant [BB/K000314/1] from the Biotechnology and Biological Sciences Research Council (to M.F.W.). Funding for open access charge:
Manica, A., Zebec, Z., Schleper, C., Zhang, J., White, M.F.   +4 more
core   +1 more source

Characterization of CRISPR-Cas Systems in Clinical Klebsiella pneumoniae Isolates Uncovers Its Potential Association With Antibiotic Susceptibility

Frontiers in Microbiology, 2018
Prokaryotic CRISPR-Cas systems limit the acquisition of genetic elements and provide immunity against invasive bacteriophage. The characteristics of CRISPR-Cas systems in clinical Klebsiella pneumoniae isolates are still unknown. Here, 97 K.
Hsin-Yu Li, Cheng-Yen Kao, Wei-Hung Lin, Po-Xing Zheng, Jing-Jou Yan, Ming-Cheng Wang, Ching-Hao Teng, Chin-Chung Tseng, Jiunn-Jong Wu   +8 more
doaj   +1 more source