Results 41 to 50 of about 75,041 (301)

Therapeutic Applications of the CRISPR-Cas System

Bioengineering, 2022
The clustered regularly interspaced palindromic repeat (CRISPR)-Cas system has revolutionized genetic engineering due to its simplicity, stability, and precision since its discovery. This technology is utilized in a variety of fields, from basic research in medicine and biology to medical diagnosis and treatment, and its potential is unbounded as new ...
Kyungmin Kang, Youngjae Song, Inho Kim, Tae-Jung Kim   +3 more
openaire   +3 more sources

Characterization of CRISPR-Cas Systems in Clinical Klebsiella pneumoniae Isolates Uncovers Its Potential Association With Antibiotic Susceptibility

Frontiers in Microbiology, 2018
Prokaryotic CRISPR-Cas systems limit the acquisition of genetic elements and provide immunity against invasive bacteriophage. The characteristics of CRISPR-Cas systems in clinical Klebsiella pneumoniae isolates are still unknown. Here, 97 K.
Hsin-Yu Li, Cheng-Yen Kao, Wei-Hung Lin, Po-Xing Zheng, Jing-Jou Yan, Ming-Cheng Wang, Ching-Hao Teng, Chin-Chung Tseng, Jiunn-Jong Wu   +8 more
doaj   +1 more source

Development and Application of CRISPR/Cas in Microbial Biotechnology

Frontiers in Bioengineering and Biotechnology, 2020
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods ...
Wentao Ding, Wentao Ding, Yang Zhang, Shuobo Shi   +3 more
doaj   +1 more source

Boosting plant immunity with CRISPR/Cas [PDF]

, 2015
CRISPR/Cas has recently been transferred to plants to make them resistant to geminiviruses, a damaging family of DNA viruses. We discuss the potential and the limitations of this method.See related Research: http://www.genomebiology.com/2015/16/1 ...
Chaparro-Garcia, Angela, Kamoun, Sophien, Nekrasov, Vladimir   +2 more
core   +1 more source

CRISPR/Cas: A powerful tool for gene function study and crop improvement

Journal of Advanced Research, 2021
Background: It is a long-standing goal of scientists and breeders to precisely control a gene for studying its function as well as improving crop yield, quality, and tolerance to various environmental stresses.
Dangquan Zhang, Zhiyong Zhang, Turgay Unver, Baohong Zhang   +3 more
doaj   +1 more source

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids.
A Bolotin, A Noy, AJR Heck, AL Gnatt, Albert J R Heck, AM Maxam, Ambrosius P L Snijders, Arjan Barendregt, B Wiedenheft, Blake Wiedenheft, C Hale, C Pourcel, CR Hale, D Han, DH Haft, DY Lee, Edze R Westra, Egbert J Boekema, Esther van Duijn, FJ Mojica, FJ Mojica, FV Karginov, GT Oostergetel, H Deveau, H Deveau, J Carte, J Li, J van der Oost, JB Ma, JE Garneau, Jelle B Bultema, Jennifer A Doudna, John van der Oost, K Lorenzen, K Yu, Kaihong Zhou, KS Makarova, LA Marraffini, LA Marraffini, LA Marraffini, M Thomas, M van Heel, M Zhou, Magnus Lundgren, Marieke R Beijer, Mark J Dickman, Matthijs M Jore, MJ Dickman, MR Singleton, N Tahallah, P Horvath, P Horvath, R Barrangou, R Jansen, RE Haurwitz, Reinhild Wurm, RH van den Heuvel, RK Lillestøl, Rolf Wagner, S Houwing, Sakharam P Waghmare, SC Raghavan, SJ Brouns, SP Waghmare, Stan J J Brouns, T Itoh, T Wilson-Sali, TA Baker, TH Tang, V Kunin, Y Agari, Ümit Pul   +71 more
core   +5 more sources

Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus

Frontiers in Microbiology, 2023
Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S.
Richard Bauer, Dorina Haider, Aline Grempels, Rebecca Roscher, Stefanie Mauerer, Barbara Spellerberg   +5 more
doaj   +1 more source

The physicist's guide to one of biotechnology's hottest new topics: CRISPR-Cas

, 2018
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, constantly evolving immune system in bacteria and archaea cells.
Bonomo, Melia E., Deem, Michael W.
core   +1 more source

CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli [PDF]

, 2013
Prokaryotes immunize themselves against transmissible genetic elements by the integration (acquisition) in clustered regularly interspaced short palindromic repeats (CRISPR) loci of spacers homologous to invader nucleic acids, defined as protospacers ...
Almendros, Cristóbal, Díez-Villaseñor, César, García-Martínez, Jesús, Guzmán, Noemí M., Mojica, Francisco J.M.   +4 more
core   +2 more sources

SnapShot: Class 1 CRISPR-Cas Systems

Cell, 2017
Class 1 CRISPR-Cas systems are characterized by effector modules consisting of multiple subunits. Class 1 systems comprise about 90% of all CRISPR-Cas loci identified in bacteria and archaea and can target both DNA and RNA.
Kira S, Makarova, Feng, Zhang, Eugene V, Koonin   +2 more
openaire   +2 more sources