Results 51 to 60 of about 18,275,652 (330)

Applications of CRISPR–Cas systems in neuroscience [PDF]

Nature Reviews Neuroscience, 2015
Genome-editing tools, and in particular those based on CRISPR-Cas (clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein) systems, are accelerating the pace of biological research and enabling targeted genetic interrogation in almost any organism and cell type. These tools have opened the door to the development of
Heidenreich, Matthias, Zhang, Feng
openaire   +4 more sources

Structure of the archaeal Cascade subunit Csa5 : Relating the small subunits of CRISPR effector complexes [PDF]

, 2013
This work was funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC) (REF: BB/G011400/1) to M.F.W. and J.H.N. and a BBSRC-funded studentship to J.R.The Cascade complex for CRISPR-mediated antiviral immunity uses CRISPR ...
Naismith, Jim, White, Malcolm F, Anderson, Linzi, Liu, Huanting, Reeks, Judith Anne, Graham, Shirley   +5 more
core   +1 more source

CRISPR-Cas Controls Cryptic Prophages

, 2022
The bacterial archetypal adaptive immune system, CRISPR-Cas, is thought to be repressed in the best-studied bacterium, Escherichia coli K-12. We show here that the E.
Severinov, Konstantin, Toshinari Maeda, Wood, Thomas K., Toshinari Maeda (76008), Konstantin Severinov, Song, Sooyeon, Sooyeon Song, Konstantin Severinov (11333), Michael J. Benedik (2353612), Michael J. Benedik, Thomas K. Wood, Laura Fernández-García, Maeda, Toshinari, Thomas K. Wood (49042), Ekaterina Semenova, Fernández-García, Laura, Laura Fernández-García (3854878), Ekaterina Semenova (13071), Benedik, Michael J., Semenova, Ekaterina, Sooyeon Song (4891132)   +20 more
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CRISPR-Cas antimicrobials: Challenges and future prospects

, 2018
Antimicrobial resistance (AMR) poses a serious threat to modern medicine and may render common infections untreatable. The discovery of new antibiotics has come to a relative standstill during the last decade [1], and developing novel approaches to ...
E Pursey (21893429), WH Gaze (21844058), D Sünderhauf (21875165), S van Houte (21852338), ER Westra (21852341)   +4 more
core   +6 more sources

Origins and evolution of CRISPR-Cas systems [PDF]

Philosophical Transactions of the Royal Society B: Biological Sciences, 2019
CRISPR-Cas, the bacterial and archaeal adaptive immunity systems, encompass a complex machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic elements (MGE), into CRISPR arrays embedded in microbial genomes. Transcripts of the inserted segments (spacers) are employed by CRISPR-Cas systems as guide (g)RNAs for ...
Eugene V. Koonin, Kira S. Makarova
openaire   +2 more sources

Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9 [PDF]

, 2015
CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high
Rojas-Fernandez, Alejandro, Hay, Ronald T., Macartney, Thomas, Herhaus, Lina, Sapkota, Gopal P., Lachaud, Christophe   +5 more
core   +1 more source

COVID-19: CRISPR/Cas-like System of nsp3 Promotes the Mutant Recombination and Drug Resistance [PDF]

, 2021
Patients with novel coronavirus pneumonia usually suffer from bacterial and fungal infections, and the drug resistance problem caused by the pandemic is becoming more and more serious. Simultaneously, the SARS-COV-2 virus has a rapid mutation phenomenon,
liu, wenzhong, Li, hualan
core   +1 more source

CRISPR/Cas: A powerful tool for gene function study and crop improvement

Journal of Advanced Research, 2021
Background: It is a long-standing goal of scientists and breeders to precisely control a gene for studying its function as well as improving crop yield, quality, and tolerance to various environmental stresses.
Dangquan Zhang, Zhiyong Zhang, Turgay Unver, Baohong Zhang   +3 more
doaj   +1 more source

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids.
Beijer, Marieke R., Mark J Dickman, Dickman, Mark J.,, Snijders, AP, Matthijs M Jore, Rolf Wagner, Sakharam P Waghmare, Beijer, Marieke, Wagner, Rolf, Brouns, Stan J. J., Barendregt, Arjan,, Snijders, Ambrosius, Dickman, Mark, Oost, J., van der, Arjan Barendregt, Boekema, Egbert, Dickman, Mark J., Bultema, J.B., Boekema, EJ, Waghmare, Sakharam P.,, Brouns, S.J.J., Wurm, Reinhild,, Westra, ER, Pul, U, Boekema, Egbert J., Doudna, Jennifer, Pul, Ümit,, Beijer, Marieke R.,, Egbert J Boekema, Jelle B Bultema, Westra, Edze, Snijders, Ambrosius P.L.,, Lundgren, Magnus, Jennifer A Doudna, Bultema, Jelle B.,, Wagner, R, John van der Oost, Westra, Edze R.,, van der Oost, J, Beijer, M.R., Wurm, R, Beijer, MR, Edze R Westra, Doudna, Jennifer A.,, Kaihong Zhou, Duijn, E., van, Jore, M.M., Heck, AJ, van der Oost, J., Waghmare, Sakharam, Jore, MM, Westra, E.R., Brouns, SJ, Doudna, Jennifer A., Wurm, Reinhild, Wagner, Rolf,, Ambrosius P L Snijders, Zhou, K, Barendregt, Arjan, Albert J R Heck, Brouns, Stan J.J.,, Magnus Lundgren, Pul, Uemit, Boekema, Egbert J.,, van Duijn, Esther, Snijders, Ambrosius P. L., Heck, Albert J. R., Lundgren, M, Pul, Ümit, Zhou, Kaihong,, Oost, John van der,, Blake Wiedenheft, Wiedenheft, Blake,, Wiedenheft, Blake, Waghmare, SP, van der Oost, John, Reinhild Wurm, Stan J J Brouns, Brouns, Stan, Jore, Matthijs M.,, Waghmare, Sakharam P., Bultema, Jelle, Zhou, Kaihong, Dickman, MJ, Lundgren, Magnus,, Barendregt, A, Bultema, JB, Jore, Matthijs, Duijn, Esther van,, Jore, Matthijs M., Lundgren, N.M.J., Ümit Pul, Marieke R Beijer, Wiedenheft, B, van Duijn, E., Heck, Albert J.R.,, Heck, Albert, Bultema, Jelle B., van Duijn, E, Westra, Edze R., Esther van Duijn, Doudna, JA   +101 more
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Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9 [PDF]

, 2014
This work was supported in part by NIH grant 1DP5OD017892 to S.LToxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the
Saima M Sidik, Sebastian Lourido, Caroline G. Hackett (226300), Hackett, Caroline G., Lourido, Sebastian, Caroline G Hackett, Saima M. Sidik (590022), Nicholas J Westwood, Nicholas J. Westwood (254891), Sidik, Saima M., Sebastian Lourido (590023), Fanny Tran, Tran, Fanny, Westwood, Nicholas J., Fanny Tran (571869)   +14 more
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