Results 61 to 70 of about 18,275,652 (330)

CRISPR-Cas-Mediated Tethering Recruits the Yeast HMR Mating-Type Locus to the Nuclear Periphery but Fails to Silence Gene Expression

, 2021
To investigate the relationship between genome structure and function, we have developed a programmable CRISPR-Cas system for nuclear peripheral recruitment in yeast.
Daniel Cunningham-Bryant, Emily R. Cliff, Robin L. Kirkpatrick (11631769), Daniel Cunningham-Bryant (6333803), Joseph L. Harman (11631775), Brianna Fernandez, Emily R. Cliff (11631766), Brianna Fernandez (11631772), Joseph L. Harman, Jesse G. Zalatan, Jesse G. Zalatan (1341795), Robin L. Kirkpatrick   +11 more
core   +1 more source

Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus

Frontiers in Microbiology, 2023
Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S.
Richard Bauer, Dorina Haider, Aline Grempels, Rebecca Roscher, Stefanie Mauerer, Barbara Spellerberg   +5 more
doaj   +1 more source

New CRISPR-Cas systems discovered [PDF]

Cell Research, 2017
In bacteria and archaea, CRISPR-Cas adaptive immune systems utilize RNA-guided endonucleases to defend against invasion by foreign nucleic acids of bacteriophage, virus and plasmid origin. In a recent paper published in Nature, Burstein et al. identified the first Cas9 protein in uncultivated archaea and two novel CRISPR-CasX and CRISPR-CasY systems in
Hui, Yang, Dinshaw J, Patel
openaire   +2 more sources

CRISPR/Cas systems: Delivery and application in gene therapy

Frontiers in Bioengineering and Biotechnology, 2022
The CRISPR/Cas systems in prokaryotes such as bacteria and archaea are the adaptive immune system to prevent infection from viruses, phages, or other foreign substances. When viruses or phages first invade the bacteria, Cas proteins recognize and cut the
Jie Huang, Yitong Zhou, Jie Li, Aiping Lu, Aiping Lu, Aiping Lu, Chao Liang, Chao Liang, Chao Liang   +8 more
doaj   +1 more source

CRISPR-Cas systems in enterococci

Brazilian Journal of Microbiology
Enterococci are members of the microbiota of humans and other animals. They can also be found in the environment, associated with food, healthcare infections, and hospital settings. Due to their wide distribution, they are inserted in the One Health context.
Amanda Seabra Cabral, Fernanda de Freitas Lacerda, Vitor Luis Macena Leite, Filipe Martire de Miranda, Amanda Beiral da Silva, Bárbara Araújo dos Santos, Jailton Lobo da Costa Lima, Lúcia Martins Teixeira, Felipe Piedade Gonçalves Neves   +8 more
openaire   +2 more sources

Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant Pseudomonas aeruginosa

mBio, 2015
Pseudomonas aeruginosa is an antibiotic-refractory pathogen with a large genome and extensive genotypic diversity. Historically, P. aeruginosa has been a major model system for understanding the molecular mechanisms underlying type I clustered regularly ...
Alex van Belkum, Leah B. Soriaga, Matthew C. LaFave, Srividya Akella, Jean-Baptiste Veyrieras, E. Magda Barbu, Dee Shortridge, Bernadette Blanc, Gregory Hannum, Gilles Zambardi, Kristofer Miller, Mark C. Enright, Nathalie Mugnier, Daniel Brami, Stéphane Schicklin, Martina Felderman, Ariel S. Schwartz, Toby H. Richardson, Todd C. Peterson, Bolyn Hubby, Kyle C. Cady   +20 more
doaj   +1 more source

Annotation and Classification of CRISPR-Cas Systems [PDF]

, 2015
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (CRISPR-associated proteins) is a prokaryotic adaptive immune system that is represented in most archaea and many bacteria. Among the currently known prokaryotic defense systems, the CRISPR-Cas genomic loci show unprecedented complexity and diversity.
Kira S, Makarova, Eugene V, Koonin
openaire   +2 more sources

An updated evolutionary classification of CRISPR–Cas systems [PDF]

Nature Reviews Microbiology, 2015
The evolution of CRISPR-cas loci, which encode adaptive immune systems in archaea and bacteria, involves rapid changes, in particular numerous rearrangements of the locus architecture and horizontal transfer of complete loci or individual modules. These dynamics complicate straightforward phylogenetic classification, but here we present an approach ...
Kira S, Makarova, Yuri I, Wolf, Omer S, Alkhnbashi, Fabrizio, Costa, Shiraz A, Shah, Sita J, Saunders, Rodolphe, Barrangou, Stan J J, Brouns, Emmanuelle, Charpentier, Daniel H, Haft, Philippe, Horvath, Sylvain, Moineau, Francisco J M, Mojica, Rebecca M, Terns, Michael P, Terns, Malcolm F, White, Alexander F, Yakunin, Roger A, Garrett, John, van der Oost, Rolf, Backofen, Eugene V, Koonin   +20 more
openaire   +3 more sources

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

CRISPR-Cas System in Antibiotic Resistance Plasmids in Klebsiella pneumoniae

Frontiers in Microbiology, 2020
CRISPR-Cas (clustered regularly interspersed short palindromic repeats-CRISPR-associated protein) is a microbial adaptive immune system involved in defense against different types of mobile genetic elements.
Muhammad Kamruzzaman, Jonathan R. Iredell, Jonathan R. Iredell   +2 more
doaj   +1 more source