Results 11 to 20 of about 45,956 (217)

Insight into the molecular mechanism of the transposon-encoded type I-F CRISPR-Cas system

Journal of Genetic Engineering and Biotechnology, 2023
CRISPR-Cas9 is a popular gene-editing tool that allows researchers to introduce double-strand breaks to edit parts of the genome. CRISPR-Cas9 system is used more than other gene-editing tools because it is simple and easy to customize.
Amnah Alalmaie, Saousen Diaf, Raed Khashan   +2 more
doaj   +1 more source

Research progress of CRISPR/Cas systems in nucleic acid detection of infectious diseases

iLABMED, 2023
Infectious diseases are a serious threat to human health, and accurate, rapid and convenient early detection of pathogens is the first step of active treatment.
Jinying Dong, Yuguang Du, Lei Zhou
doaj   +1 more source

Research Progress on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)-Isothermal Amplification in the Detection of Foodborne Pathogens [PDF]

Shipin Kexue
Food safety incidents caused by foodborne pathogens have attracted widespread attention. In order to ensure food safety, it is essential to adopt effective detection means.
ZHANG Qi, PANG Lidong, SU Qunchao, SONG Danliangmin, YANG Xinyan, JIANG Yujun, ZHANG Wei
doaj   +1 more source

Phylogenetic Distribution of CRISPR-Cas Systems in Staphylococcus lugdunensis

Microbiology Spectrum, 2021
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) genes (CRISPR-Cas) are present in many bacterial genomes with functions beyond adaptive immunity.
Cheng-Yen Kao, Jang-Jih Lu, Lee-Chung Lin, Hsiao-Chi Lin, Shih-Cheng Chang   +4 more
doaj   +1 more source

Disabling a Type I-E CRISPR-Cas Nuclease with a Bacteriophage-Encoded Anti-CRISPR Protein

mBio, 2017
CRISPR (clustered regularly interspaced short palindromic repeat)-Cas adaptive immune systems are prevalent defense mechanisms in bacteria and archaea.
April Pawluk, Megha Shah, Marios Mejdani, Charles Calmettes, Trevor F. Moraes, Alan R. Davidson, Karen L. Maxwell   +6 more
doaj   +1 more source

Characterization of 67 Confirmed Clustered Regularly Interspaced Short Palindromic Repeats Loci in 52 Strains of Staphylococci

Frontiers in Microbiology, 2021
Staphylococcus aureus (S. aureus), which is one of the most important species of Staphylococci, poses a great threat to public health. Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) are an ...
Ying Wang, Tingting Mao, Yinxia Li, Wenwei Xiao, Xuan Liang, Guangcai Duan, Haiyan Yang   +6 more
doaj   +1 more source

Cas1 and Cas2 From the Type II-C CRISPR-Cas System of Riemerella anatipestifer Are Required for Spacer Acquisition

Frontiers in Cellular and Infection Microbiology, 2018
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins provide acquired genetic immunity against the entry of mobile genetic elements (MGEs).
Yang He, Yang He, Yang He, Mingshu Wang, Mingshu Wang, Mingshu Wang, Mafeng Liu, Mafeng Liu, Mafeng Liu, Li Huang, Li Huang, Li Huang, Chaoyue Liu, Chaoyue Liu, Chaoyue Liu, Xin Zhang, Xin Zhang, Xin Zhang, Haibo Yi, Haibo Yi, Haibo Yi, Anchun Cheng, Anchun Cheng, Anchun Cheng, Dekang Zhu, Dekang Zhu, Qiao Yang, Qiao Yang, Qiao Yang, Ying Wu, Ying Wu, Ying Wu, Xinxin Zhao, Xinxin Zhao, Xinxin Zhao, Shun Chen, Shun Chen, Shun Chen, Renyong Jia, Renyong Jia, Renyong Jia, Shaqiu Zhang, Shaqiu Zhang, Shaqiu Zhang, Yunya Liu, Yunya Liu, Yunya Liu, Yanling Yu, Yanling Yu, Yanling Yu, Ling Zhang, Ling Zhang, Ling Zhang   +52 more
doaj   +1 more source

The virulence factor regulator and quorum sensing regulate the type I-F CRISPR-Cas mediated horizontal gene transfer in Pseudomonas aeruginosa

Frontiers in Microbiology, 2022
Pseudomonas aeruginosa is capable of thriving in diverse environments due to its network of regulatory components for effective response to stress factors.
Stephen Dela Ahator, Stephen Dela Ahator, Yang Liu, Yang Liu, Jianhe Wang, Lian-Hui Zhang   +5 more
doaj   +1 more source

Determination of Acr-mediated immunosuppression in Pseudomonas aeruginosa

MethodsX, 2023
Bacteria have a broad array of defence mechanisms to fight bacteria-specific viruses (bacteriophages, phages) and other invading mobile genetic elements. Among those mechanisms, the ‘CRISPR-Cas’ (Clustered Regularly Interspaced Short Palindromic Repeats –
Benoit J. Pons, Edze R. Westra, Stineke van Houte   +2 more
doaj   +1 more source

DNA Nanomaterials for Delivery of Clustered Regularly Interspaced Short Palindromic Repeats/Cas Systems

Small Structures, 2023
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein (CRISPR/Cas) system has been exploited as an efficient gene editing tool with precise site specificity and high efficiency; the delivery of the CRISPR/Cas system is
Zhaoyue Lv, Zhemian Li, Peiran Li, Chi Yao, Dayong Yang   +4 more
doaj   +1 more source