Results 11 to 20 of about 75,041 (301)
New CRISPR-Cas systems discovered [PDF]
Cell Research, 2017 In bacteria and archaea, CRISPR-Cas adaptive immune systems utilize RNA-guided endonucleases to defend against invasion by foreign nucleic acids of bacteriophage, virus and plasmid origin. In a recent paper published in Nature, Burstein et al. identified the first Cas9 protein in uncultivated archaea and two novel CRISPR-CasX and CRISPR-CasY systems in
Hui, Yang, Dinshaw J, Patel
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Research advances on pathogenic nucleic acid detection technology based on CRIPSR/Cas system
浙江大学学报. 农业与生命科学版, 2023 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) (CRISPR/Cas) system, an ancient bacterial and archaeal immune system, has rapidly developed into a popular gene-editing tool, which largely promotes the ...
LI Hongzhao +4 more
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CRISPR-Cas systems in multicellular cyanobacteria [PDF]
RNA Biology, 2018 Novel CRISPR-Cas systems possess substantial potential for genome editing and manipulation of gene expression. The types and numbers of CRISPR-Cas systems vary substantially between different organisms. Some filamentous cyanobacteria harbor > 40 different putative CRISPR repeat-spacer cassettes, while the number of cas gene instances is much lower ...
Shengwei Hou +6 more
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CASPredict: a web service for identifying Cas proteins [PDF]
PeerJ, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated (Cas) proteins constitute the CRISPR-Cas systems, which play a key role in prokaryote adaptive immune system against invasive foreign elements.
Shanshan Yang, Jian Huang, Bifang He
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Mechanisms regulating the CRISPR-Cas systems
Frontiers in Microbiology, 2023 The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats- CRISPR associated proteins) is a prokaryotic system that enables sequence specific recognition and cleavage of nucleic acids. This is possible due to cooperation between CRISPR array which contains short fragments of DNA called spacers that are complimentary to the targeted ...
Marta Zakrzewska +3 more
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Frontiers in Plant Science, 2022 Different genome editing approaches have been used to engineer resistance against plant viruses. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas; CRISPR/Cas) systems to create pinpoint genetic ...
Fredy D. A. Silva +1 more
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CRISPR-Cas systems in Proteus mirabilis
Infection, Genetics and Evolution, 2021 The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a bacterial defense mechanism against bacteriophages composed of two different parts: the CRISPR array and the Cas genes. The spacer acquisition is done by the adaptation module consisting of the hallmark Cas1 Cas2 proteins, which inserts new spacers into the CRISPR array.
Mahnaz Shafaei, Fallah +3 more
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Insight into the molecular mechanism of the transposon-encoded type I-F CRISPR-Cas system
Journal of Genetic Engineering and Biotechnology, 2023 CRISPR-Cas9 is a popular gene-editing tool that allows researchers to introduce double-strand breaks to edit parts of the genome. CRISPR-Cas9 system is used more than other gene-editing tools because it is simple and easy to customize.
Amnah Alalmaie +2 more
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Using CRISPR-Cas systems as antimicrobials [PDF]
Current Opinion in Microbiology, 2017 Although CRISPR-Cas systems naturally evolved to provide adaptive immunity in bacteria and archaea, Cas nucleases can be co-opted to target chromosomal sequences rather than invasive genetic elements. Although genome editing is the primary outcome of self-targeting using CRISPR-based technologies in eukaryotes, self-targeting by CRISPR is typically ...
Bikard, David, Barrangou, Rodolphe
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Development and Application of CRISPR/Cas System in Rice
Rice Science, 2019 In the past several years, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system has been harnessed as an efficient and powerful tool for targeted genome editing in different prokaryotic and ...
Ren Jun +3 more
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