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Regulation of CRISPR–Cas adaptive immune systems
Current Opinion in Microbiology, 2017CRISPR-Cas systems are prokaryotic immune systems that allow defense against invasion by foreign genetic elements. Through the acquisition of genetic memory representing prior exposure, the CRISPR-Cas machinery identifies complementary nucleic acids and mediates their destruction.
Adrian G, Patterson +2 more
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CRISPR-Cas systems: Challenges and future prospects
2021The advancement gained over the past couple of decades in clustered regularly interspaced short palindromic repeats and CRISPR associated proteins (CRISPR-Cas) systems have revolutionized the field of synthetic biology, therapeutics, diagnostics and metabolic engineering. The technique has enabled the process of genome editing to be very precise, rapid,
Nisarg, Gohil +4 more
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Engineering virus resistance via CRISPR–Cas systems
Current Opinion in Virology, 2018In prokaryotes, CRISPR/Cas adaptive immunity systems target and destroy nucleic acids derived from invading bacteriophages and other foreign genetic elements. In eukaryotes, the native function of these systems has been exploited to combat viruses in mammals and plants.
Ahmed Mahas, Magdy Mahfouz
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Engineering Translational Activators with CRISPR-Cas System
ACS Synthetic Biology, 2015RNA parts often serve as critical components in genetic engineering. Here we report a design of translational activators which is composed of an RNA endoribonuclease (Csy4) and two exchangeable RNA modules. Csy4, a member of Cas endoribonuclease, cleaves at a specific recognition site; this cleavage releases a cis-repressive RNA module (crRNA) from the
Pei, Du +4 more
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CRISPR/Cas Systems towards Next-Generation Biosensing
Trends in Biotechnology, 2019Beyond its remarkable genome editing ability, the CRISPR/Cas9 effector has also been utilized in biosensing applications. The recent discovery of the collateral RNA cleavage activity of the Cas13a effector has sparked even greater interest in developing novel biosensing technologies for nucleic acid detection and promised significant advances in CRISPR
Yi, Li +3 more
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Gene regulation by engineered CRISPR-Cas systems
Current Opinion in Microbiology, 2014The clustered regularly interspaced short palindromic repeat (CRISPR) arrays and their CRISPR associated (Cas) proteins constitute adaptive immune systems in bacteria and archaea that provide protection from bacteriophages, plasmids and other mobile genetic elements (MGEs).
Peter C, Fineran, Ron L, Dy
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CRISPR‐Cas systems and RNA‐guided interference
WIREs RNA, 2013AbstractClustered regularly interspaced short palindromic repeats (CRISPR) together with associated sequences (cas) form the CRISPR‐Cas system, which provides adaptive immunity against viruses and plasmids in bacteria and archaea. Immunity is built through acquisition of short stretches of invasive nucleic acids into CRISPR loci as ‘spacers'.
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Functionally diverse type V CRISPR-Cas systems
Science, 2019Additional, diverse CRISPR systems CRISPR systems have been revolutionizing molecular biology. Mining the metagenomic database, Yan et al. systematically discovered additional subtypes of type V CRISPR-Cas systems.
Winston X. Yan +11 more
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Advances in CRISPR-Cas systems for epigenetics
The CRISPR-Cas9 method has revolutionized the gene editing. Epigenetic changes, including DNA methylation, RNA modification, and changes in histone proteins, have been intensively studied and found to play a key role in the pathogenesis of human diseases.Mahnoor, Ilyas +6 more
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Efficient computation reveals rare CRISPR–Cas systems
Nature Reviews Genetics, 2023openaire +2 more sources