Results 291 to 300 of about 58,663 (324)

Exploring the Frozen Armory: Antiphage Defense Systems in Cold-Adapted Bacteria with a Focus on CRISPR-Cas Systems. [PDF]

Microorganisms
Sandsdalen GD, Kumar A, Hjerde E.
europepmc   +1 more source

Achieving scalable expansion of therapeutic porcine hepatocytes in vivo through serial transplantation

Animal Models and Experimental Medicine, EarlyView.
This represents the first serial hepatocyte transplantation pig model designed to investigate the massive hepatocyte proliferation in vivo. Leveraging this novel model, we achieved serial hepatocyte transplantation and regeneration in a porcine model, where the transplanted hepatocytes effectively repopulated the livers of two‐generation model pigs in ...
Zhiqiang Han, Xin Wang, Dawei Yu, Jing Wang, Ke Sun, Siqi Wang, Ying Zhang, Guihai Feng, Wei Li, Tang Hai, Jilong Ren   +10 more
wiley   +1 more source

A semi-lethal CRISPR-Cas system permits DNA acquisition in Enterococcus faecalis

, 2017
Karthik Hullahalli, Marinelle Rodrigues, Uyen Thy Nguyen, Kelli L. Palmer   +3 more
openalex   +1 more source

Detection and Analysis of CRISPR-Cas System Structures in Genome of Plasmid pYC-1 of Bacillus thuringiensis Strain YC-10

, 2018
N. A. Arefieva, Yu. P. Dzhioev, A. Yu. Borisenko, Л. А. Степаненко, N. P. Peretolchina, Yu. S. Bukin, В. И. Чемерилова, O. F. Vyatchina, О. А. Секерина, Yu. A. Markova, G. V. Yurinova, В. П. Саловарова, А. А. Приставка, V. A. Kuzminova, A. S. Martynova, В. И. Злобин   +15 more
openalex   +1 more source

Occurrence and diversity pattern of CRISPR-Cas systems in Acetobacter genus provides insights on adaptive defense mechanisms against to invasive DNAs. [PDF]

Front Microbiol
Ghaffarian S, Panahi B.
europepmc   +1 more source

Graphene Oxide‐Based Gene Modulation in Preferential Elimination of Lung Cancer Cells in a 3D Tumor Microenvironment Model

Advanced NanoBiomed Research, EarlyView.
Chemically modified graphene oxide nanocarriers provide a targeted lung cancer gene therapy platform. In a 3D tumor microenvironment model, these nanocarriers deliver therapeutic genes, regulate immune markers, and induce apoptosis in cancer cells. Combined gene delivery enhances cancer cell elimination, demonstrating the potential of this approach for
Francesca Grilli, Sadman Sakib, Fabio Variola, Shan Zou   +3 more
wiley   +1 more source

Engineered Hydrogels Revolutionize Locoregional Cancer Immunotherapy

Advanced NanoBiomed Research, EarlyView.
This review systematically summarizes the latest research progress on injectable hydrogels in cancer immunotherapy, highlighting their unique advantages in precise immune activation, local drug‐controlled release, and immune microenvironment remodeling. These features provide innovative solutions to overcome the bottlenecks of systemic side effects and
Jiyong Wei, Chunliu Huang, Zenghua Zhou, Yanni Lan   +3 more
wiley   +1 more source

Nanoparticles Functionalized with Polymer Brushes for Gene Delivery

Advanced NanoBiomed Research, EarlyView.
The engineering of the next generation gene delivery vectors requires the precise control of the architecture and physicochemical interactions controlling the transport, targeting, uptake, and release of nucleic acid materials to targeted cells. Polymer brush‐functionalized nanomaterials offer unprecedented control over such design and properties, and ...
Carlos E. Neri‐Cruz, Julien E. Gautrot
wiley   +1 more source

PCR‐Free Site‐Directed Mutagenesis on Repetitive Sequences Using Single‐Stranded DNA‐Assisted Double‐Stranded DNA Nicking by DNAzymes

Angewandte Chemie International Edition, Accepted Article.
Site‐directed mutagenesis (SDM) is a key driver for many biochemical investigations and biotechnological applications. Despite decades of development, it is still difficult to perform SDM on some sequences, including highly repetitive ones, because polymerase chain reaction (PCR) is often used as the most effective method for SDM, but results in ...
Mingkuan Lyu, Linggen Kong, Xiangli Shao, Yi Lu   +3 more
wiley   +1 more source