Results 301 to 310 of about 713,183 (311)

RNA-targeting CRISPR–Cas systems

Nature Reviews Microbiology, 2022
CRISPR-Cas is a widespread adaptive immune system in bacteria and archaea that protects against viral infection by targeting specific invading nucleic acid sequences. Whereas some CRISPR-Cas systems sense and cleave viral DNA, type III and type VI CRISPR-Cas systems sense RNA that results from viral transcription and perhaps invasion by RNA viruses ...
Sam P. B. van Beljouw, Jasper Sanders, Alicia Rodríguez-Molina, Stan J. J. Brouns   +3 more
openaire   +2 more sources

CRISPR/Cas System: An Introduction

2021
The process of altering genetic code to modify DNA precisely and efficiently within a cell is known as genome editing. All techniques used for changing a gene in a cell are based on cutting of double-stranded DNA through DNA endonucleases at specific site.
Aftab Ahmad, Nayla Munawar
openaire   +2 more sources

Enhancing CRISPR/Cas systems with nanotechnology

Trends in Biotechnology, 2023
CRISPR/Cas systems have revolutionized biology and medicine, and have led to new paradigms in disease diagnostics and therapeutics. However, these complexes suffer from key limitations regarding barriers to cellular entry, stability in biological environments, and off-target effects.
Rupali Chowdhry, Steven Z. Lu, Seungheon Lee, Shivudu Godhulayyagari, Sasha B. Ebrahimi, Devleena Samanta   +5 more
openaire   +2 more sources

Current understanding of the cyanobacterial CRISPR-Cas systems and development of the synthetic CRISPR-Cas systems for cyanobacteria

Enzyme and Microbial Technology, 2020
Cyanobacteria are photosynthetic microorganisms that are capable of converting CO2 to value-added chemicals. Engineering of cyanobacteria with synthetic biology tools, including the CRISPR-Cas system, has allowed an opportunity for biological CO2 utilization.
Napisa Pattharaprachayakul, Mieun Lee, Aran Incharoensakdi, Han Min Woo   +3 more
openaire   +3 more sources

The CRISPR/Cas System in Human Cancer

2023
The use of CRISPR as a genetic editing tool modified the oncology field from its basic to applied research for opening a simple, fast, and cheaper way to manipulate the genome. This chapter reviews some of the major uses of this technique for in vitro- and in vivo-based biological screenings, for cellular and animal model generation, and new derivative
Martín Hérnan, Bonamino, Eduardo Mannarino, Correia   +1 more
openaire   +2 more sources

An introduction and use of the CRISPR-Cas systems

2021
Clusters of regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated proteins (Cas) system (CRISPR-Cas) is a rapidly evolving field of targeted genome engineering. The type II CRISPR-Cas9 is used for genome editing of many organisms. Single guide RNA (sgRNA) can bind to Cas9 protein that can target desired sequences in presence of
openaire   +3 more sources

CRISPR-Cas system and coevolution of bacteria and phages

Hereditas (Beijing), 2011
In response to the challenge from phages, bacteria evolve a number of defense systems against phage invasion. Meanwhile, the phages evolve multiple counter-defense mechanism as well under the selection pressure from bacteria. The evolution relationship between bacteria and phages, as well as the functional mechanism, still remains to be uncovered.
Bo Du, Tie-Min Li
openaire   +3 more sources

The CRISPR-Cas system in molecular diagnostics

Clinica Chimica Acta
Robust, sensitive, and rapid molecular detection tools are essential prerequisites for disease diagnosis and epidemiological control. However, the current mainstream tests necessitate expensive equipment and specialized operators, impeding the advancement of molecular diagnostics. The CRISPR-Cas system is an integral component of the bacterial adaptive
Rao, Song, Zhongyi, Chen, Hongtao, Xiao, Haojun, Wang   +3 more
openaire   +2 more sources

Advances in CRISPR-Cas systems for epigenetics

The CRISPR-Cas9 method has revolutionized the gene editing. Epigenetic changes, including DNA methylation, RNA modification, and changes in histone proteins, have been intensively studied and found to play a key role in the pathogenesis of human diseases.
Mahnoor, Ilyas, Qasim, Shah, Alvina, Gul, Huzaifa, Ibrahim, Rania, Fatima, Mustafeez Mujtaba, Babar, Jayakumar, Rajadas   +6 more
openaire   +2 more sources

The CRISPR/Cas system in zebrafish

2020
De Santis, Flavia, Terriente, Javier, Di Donato, Vincenzo   +2 more
openaire   +1 more source