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Statement of Retraction: Easy efficient HDR-based targeted knock-in in <i>Saccharomyces cerevisiae</i> genome using CRISPR-Cas9 system. [PDF]
Bioengineeredeuropepmc +1 more source
Correction to "CRISPR/Cas9 screen in human iPSC-derived cortical neurons identifies NEK6 as a novel disease modifier of C9orf72 poly(PR) toxicity". [PDF]
Alzheimers Dementeuropepmc +1 more source
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2021
The gene scissors CRISPR/Cas9 has revolutionized genetic engineering in many ways. The technology promises to precisely cut and change gene segments from humans, animals and plants. But how should we deal with a technology that could irreversible change the genetic make-up? A public debate is of utmost importance to answer what science is allowed to do.
Reagan Mudziwapasi +15 more
+6 more sources
The gene scissors CRISPR/Cas9 has revolutionized genetic engineering in many ways. The technology promises to precisely cut and change gene segments from humans, animals and plants. But how should we deal with a technology that could irreversible change the genetic make-up? A public debate is of utmost importance to answer what science is allowed to do.
Reagan Mudziwapasi +15 more
+6 more sources
Neuron, 2023
Genome engineering technologies provide an entry point into understanding and controlling the function of genetic elements in health and disease. The discovery and development of the microbial defense system CRISPR-Cas yielded a treasure trove of genome engineering technologies and revolutionized the biomedical sciences.
Georgios Kalamakis, Randall J. Platt
openaire +2 more sources
Genome engineering technologies provide an entry point into understanding and controlling the function of genetic elements in health and disease. The discovery and development of the microbial defense system CRISPR-Cas yielded a treasure trove of genome engineering technologies and revolutionized the biomedical sciences.
Georgios Kalamakis, Randall J. Platt
openaire +2 more sources
2023
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +2 more sources
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +2 more sources
CRISPR Critters and CRISPR Cracks
The American Journal of Bioethics, 2015This essay focuses on possible nonhuman applications of CRISPR/Cas9 that are likely to be widely overlooked because they are unexpected and, in some cases, perhaps even "frivolous." We look at five uses for "CRISPR Critters": wild de-extinction, domestic de-extinction, personal whim, art, and novel forms of disease prevention.
R Alta, Charo, Henry T, Greely
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Science, 2021
New CRISPR enzyme activities add to the nucleic acid detection ...
Omar O, Abudayyeh +1 more
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New CRISPR enzyme activities add to the nucleic acid detection ...
Omar O, Abudayyeh +1 more
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CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics
Angewandte Chemie International Edition, 2023AbstractPolymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires a long turnaround time, expensive equipment, and trained personnel, limiting its widespread availability and diagnostic capacity.
Zhengyan Weng +7 more
openaire +2 more sources
2017
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +2 more sources
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +2 more sources