Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Enhanced Tangential Flow Filtration of Precipitated Proteins Using Screened Membrane Cassettes. [PDF]
Membranes (Basel)Badinger Z, Behboudi A, Zydney AL.
europepmc +1 more source
Flow cytometric measurement of cytoplasmic pH: A critical evaluation of available fluorochromes [PDF]
, 1986 Elizabeth A. Musgrove +2 more
openalex +1 more source
Recent developments in organic redox flow batteries: A critical review
, 2017 P. Leung +8 more
semanticscholar +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
CO2, infections, and critical illnesses. [PDF]
Trauma Surg Acute Care OpenColeman LS.
europepmc +1 more source
Study of safety relief valve operation under ATWS conditions. [Super critical flow]
, 1979 E.S. Hutmacher, S.D. Whitten
openalex +2 more sources
Particle And Flow Field Holography A Critical Survey [PDF]
, 1985 James D. Trolinger
openalex +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Mean Airway Pressure-An Informative but Overlooked Indicator of Mechanical Power. [PDF]
Crit Care ExplorMichalik MG, Crooke PS, Marini JJ.
europepmc +1 more source