Results 71 to 80 of about 8,721,470 (292)

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Estimating Discharge From Undular Hydraulic Jumps: Feasibility Assessment Based on Flume Experiments

open access: yesWater Resources Research
Rapids are common in steep rivers, often forming where flow transitions from supercritical (Froude number, Fr > 1) to subcritical (Fr 
Daniel C. White   +8 more
doaj   +1 more source

Long-range, critical-point dynamics in oil field flow rate data [PDF]

open access: yes, 2006
Earthquake triggering data exhibit long-range spatio-temporal correlations of the power-law form C(l) ∼ l−α and anomalously-slow temporal diffusion of the mean triggering distance of the form: 〈l〉 ∼ tH, with H < 0.5.
Papasouliotis, O.   +6 more
core   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

open access: yesFEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar   +4 more
wiley   +1 more source

Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota

open access: yesNature Communications, 2020
Bioorthogonal non-canonical amino acid tagging (BONCAT) coupled with flow cytometry (FACS) has been utilized to profile active microbiome of environmental samples.
Talia D. Valentini   +6 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

open access: yesFEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik   +5 more
wiley   +1 more source

Large Eddy Simulation of the Flow Past a Circular Cylinder at Super-Critical Reynolds Numbers

open access: yes, 2020
Turbulent flow past a circular cylinder at super-critical Reynolds numbers is simulated using large eddy simulation in this study. A novel combination of O- and H-grid structures is used to reduce mesh cells and, in turn, the computational cost.
Yang, Z., Ahmadi, M.
core  

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

High Accuracy Volume Flow Rate Measurement Using Vortex Counting [PDF]

open access: yes, 2013
A prototype device for measuring the volumetric flow-rate by counting vortices has been designed and realized. It consists of a square-section pipe in which are placed a two-dimensional bluff body and a strain gauge force sensor.
RAVELET, Florent   +3 more
core   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

open access: yesFEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang   +4 more
wiley   +1 more source

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