Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Climate-stressed crop mycobiomes as pre-harvest sentinels of human mycotoxin exposure: an AI-enabled One Health framework linking dysbiosis, exposomics, and toxicopathology. [PDF]
Das SK, Karak P, Parvatikar P.
europepmc +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
ArqPy: A Python Toolbox for Remote Sensing Image Preprocessing and AI-Assisted Interpretation of Derived Products for Archaeological Prospection. [PDF]
Iranzo C +3 more
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Perennial and annual cropping sequences differentially influence soil functionality in Luvisolic soils. [PDF]
Bhattarai K +7 more
europepmc +1 more source
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Editorial: Plant-virus interactions: crop resistance in focus
Jiban Kumar Kundu +3 more
doaj +1 more source
Factors affecting the infestation status of African sugarcane stalk borer (Eldana saccharina Walker) (Lepidoptera: Pyralidae) in Tanzania. [PDF]
Wambura HD, Rwegasira GM, Martin MJ.
europepmc +1 more source

