Results 301 to 310 of about 170,253 (336)

[33] Membrane-impermeant cross-linking reagents

1989
Publisher Summary Membrane-impermeant cross-linking reagents based on sulfosuccinimidyl ester chemistry have made accessible several new approaches to the study of membrane-protein structure and function. The impermeant character of these reagents allows an experimenter not only to study the subunits of an oligomeric membrane protein that are ...
James V. Staros, P. S. R. Anjaneyulu
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Synthesis of “long,” hydrophilic, protein-cross-linking reagents

Analytical Biochemistry, 1974
Abstract Since most of the protein cross-linking reagents in use are strongly hydrophobic, their length cannot be increased beyond approximately 20 A between the protein-reactive groups, before denaturation of most proteins becomes noticeable at already a very few cross-links per molecule.
K. Wetz, H. Fasold, Ch. Meyer
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A new nucleic acid-protein cross-linking reagent

Molecular and General Genetics MGG, 1977
A new photoactivable reagent is described, which allows the formation of RNA-protein cross-links via disulfide bridges in combination with mercaptobutyrimidate. The reconstituted L24 protein-23S RNA complex from the large subunit of E. coli ribosomes has been used as a model system for the cross-linking.
Oste, Christian, Parfait, Raymond, Bollen, Alex, Crichton, Robert R.R.   +3 more
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An amino acid-based heterofunctional cross-linking reagent

Amino Acids, 2014
We describe the synthesis and characterization of a new lysine-based heterofunctional cross-linking reagent. It carries two readily available aminooxy functionalities and an activated and protected thiol group that is capable of generating reducible disulfides, the former enable bioorthogonal modification of ketones and aldehydes by the formation of an
Kalina Peneva, Marco Lelle
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Accessibility and cross-linking of native neurofilaments to chemical reagents

International Journal of Biochemistry, 1984
Native neurofilaments were submitted to cross-linking reactions with bifunctional reagents (DMA, DMS and DSS) and to chemical reactions with sterically bulky reagents such as EEDQ and DTAF , as well as a glutaraldehyde-activated gel. The 160K and 70K neurofilament proteins reacted slightly more than the 210K neurofilament protein with DMS and DSS.
Kia-Ki Han, André Delacourte, M. Mazzuca, Catherine Francois   +3 more
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Novel DNA Cross-Linking Reagents

2016
Abstract DNA interstrand cross-links (ICLs) are the sources of the cytotoxicity of many anticancer agents. DNA cross-linking agents are used as anticancer agents, for DNA damage and repair study, for nucleic acid detection, and for construction of DNA nanomaterials.
Xiaohua Peng, Heli Fan
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Cross-linking reagents and membrane organization

Trends in Biochemical Sciences, 1979
Abstract Bifunctional cross-linking reagents have been widely used to study quaternary structure in oligomeric enzymes — their application to membrane systems involves a new level of problems but is proving informative.
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Chemical cross-links of proteins by using bifunctional reagents

International Journal of Biochemistry, 1984
Abstract 1. 1. The chemical identification of spatial arrangements of the subunits in oligomeric proteins is exclusively achieved by the analysis of the reaction products of the protein and bifunctional reagents. 2. 2. Since the pioneer work of Hartman and Wold ( Biochemistry 6 , 2439–2448, 1967) the bifunctional reagent such as bis-imido ...
Kia-Ki Han, Claude Richard, André Delacourte   +2 more
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Chemical Cross-Linking: Reagents and Problems in Studies of Membrane Structure

Annual Review of Biochemistry, 1977
INTRODUCTION AND PERSPECTIVE ....... 524 THE PROCEDURE AND THE PROBLEMS. . . . . .... ...... . . .. . ... . . .... .... . 525 CHEMISTRY OF SELECTED REAGENTS 527 Alkyl Imidates (lmidoesters} 527 Synthesis 528 Reaction paths and mechanisms 528 Use as modifYing reagents 531 Acyl Azides 532 Sulfhydryl Reagents 532 Nitrenes 534 ...
Kevin Peters, Frederic M. Richards
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A new cleavable reagent for cross-linking and reversible immobilization of proteins

Biochemical and Biophysical Research Communications, 1979
Abstract We have prepared a new bifunctional reagent for the cross-linking and reversible immobilization of proteins through their amine groups. This compound, ethylene glycolyl bis(succinimidyl succinate), reacts rapidly with proteins, at pH 7 and at high dilution.
Garfield P. Royer, Paul K. Smith, Peter M. Abdella   +2 more
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