Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Valorisation of glycerol by Pichia pastoris under increased air pressure [PDF]
Pichia pastoris has many biotechnological applications. Two aspects of the species have contributed to its utility: (1) fermentation techniques were developed for maintaining extremely high cell densities in excess of 100 g/L dry weight, and (2) because ...
Belo, Isabel, Lopes, Marlene, Mota, M.
core
Inhibition of Fructose-1,6-bisphosphatase by Aminoimidazole Carboxamide Ribotide Prevents Growth of Salmonella enterica purH Mutants on Glycerol [PDF]
The enzyme fructose-1,6-bisphosphatase (FBP) is key regulatory point in gluconeogenesis. Mutants of Salmonella enterica lacking purH accumulate 5-amino-4-imidazole carboxamide ribotide (AICAR) and are unable to utilize glycerol as sole carbon and energy ...
Boyd, Jeffrey M. +2 more
core +1 more source
Harnessing Fungal Biowelding for Constructing Mycelium‐Engineered Materials
Mycelium‐bound composites (MBCs) offer low‐carbon alternatives for construction, yet interfacial bonding remains a critical challenge. This review examines fungal biowelding as a biocompatible adhesive, elucidating mycelium‐mediated interfacial mechanisms and their role in material assembly. Strategies to optimize biowelding are discussed, highlighting
Xue Brenda Bai +2 more
wiley +1 more source
Anaerobic digestion of crude glycerol for biohydrogen production [PDF]
Biodiesel has become one of the main interests for the diesel replacement because it is renewable, and can be produced from various sources. However, the rapid acceleration of biodiesel production consequently contributes towards the mass amount of ...
Ahmad, Mohd. Azlan
core
Anaerobic co-digestion of residues from the cold pressing and trans-esterification of oilseed rape (OSR) with other farm wastes was considered as a means of enhancing the sustainability of on-farm biodiesel production.
Banks, Charles J. +2 more
core +1 more source
Discovery of macrocyclic inhibitors of apurinic/apyrimidinic endonuclease 1 [PDF]
Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential base excision repair enzyme that is upregulated in a number of cancers, contributes to resistance of tumors treated with DNA-alkylating or -oxidizing agents, and has recently been identified as ...
Beglov, Dmitri +12 more
core +7 more sources
Packaging of Macroscopic Material Payloads: Needs, Challenges, Concepts, and Future Directions
This review introduces a unified framework that decomposes any macroscopic packaging system into the payload, packaging material, and packaging strategy and combines them into a conceptual packaging equation: packaging strategy = payload + packaging material.
Venkata S. R. Jampani, Manos Anyfantakis
wiley +1 more source
Beech wood treated with polyglycerol succinate: a new effective method for its protection and stabilization [PDF]
This paper deals with an original and non-biocidal chemical treatment consisting of a vacuum/pressure impregnation step of beech wood with a water-borne mixture made from heat-activated condensation of succinic anhydride (SA) and glycerol (G).
Fredon, Emmanuel +4 more
core +1 more source
Laser‐Induced Graphene from Waste Almond Shells
Almond shells, an abundant agricultural by‐product, are repurposed to create a fully bioderived almond shell/chitosan composite (ASC) degradable in soil. ASC is converted into laser‐induced graphene (LIG) by laser scribing and proposed as a substrate for transient electronics.
Yulia Steksova +9 more
wiley +1 more source

