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Cryoelectron microscopy of refrozen cryosections
Journal of Structural Biology, 2003Cryoelectron microscopy makes it possible to record high-resolution detail from large and complex structures. However, its application to understanding cellular structure is limited by the requirement that samples should be no thicker than approximately 0.5-1 microm.
Pradeep K Luther, Edward P Morris
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iMEM: Isolation of Plasma Membrane for Cryoelectron Microscopy [PDF]
Structure, 2016 The plasma membrane and the cell cortex are essential parts of the eukaryotic cell. The plasma membrane delimitates the cell and mediates communication with the outside. The cell cortex is the submembrane cytoskeleton shaping the cell and is able to reorganize for the passage of material.
Sven Beckmann, Benoit Zuber
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Cryoelectron microscopy and cryoelectron tomography of the nuclear pre-mRNA processing machine
Journal of Structural Biology, 2002Large nuclear ribonucleoprotein particles, which can be viewed as the naturally assembled precursor messenger RNA (pre-mRNA) processing machine, were analyzed in frozen-hydrated preparations by cryoelectron microscopy. A general and reproducible strategy for preparing ice-embedded large nuclear ribonucleoprotein (lnRNP) particles at sufficiently high ...
Ohad Medalia +2 more
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Cryoelectron microscopy of macromolecular complexes
Biology of the Cell, 1994Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X‐ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies.
R H, Wade, E A, Hewat
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Cryoelectron Microscopy of Fission Yeast
Cold Spring Harbor Protocols, 2017Fission yeast cells can be prepared for electron microscopy (EM) in the frozen-hydrated state. This eliminates the requirement for dehydration and heavy metal staining when preparing samples for EM. As with room temperature imaging, however, the yeast must be sectioned to make them thin enough for transmission of the electron beam.
Mary K, Morphew +2 more
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Cryoelectron microscopy of vitrified Sendai virions
Journal of Virological Methods, 1988Morphology of vitrified Sendai virions was studied by transmission type electron microscopy. Almost all the virions appeared to be completely spherical, although their diameters differed. A possibly continuously long nucleocapsid was seen running helically in an envelope. Spikes were seen on the virion surfaces.
Y, Hosaka, T, Watabe
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Cryoelectron Microscopy of Liposomes
2005A thin aqueous film of suspended lipid vesicles?micelles is the object of choice for vitrification and subsequent study by cryoelectron microscopy. Just prior to vitrification, a thin film (compare with a soap film) is vulnerable to heat and mass exchange.
Peter M, Frederik, D H W, Hubert
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Automated Identification of Filaments in Cryoelectron Microscopy Images
Journal of Structural Biology, 2001Since the foundation for the three-dimensional image reconstruction of helical objects from electron micrographs was laid more than 30 years ago, there have been sustained developments in specimen preparation, data acquisition, image analysis, and interpretation of results.
Bridget Carragher +2 more
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Virus structures revealed by advanced cryoelectron microscopy methods
Structure, 2023Before the resolution revolution, cryoelectron microscopy (cryo-EM) single-particle analysis (SPA) already achieved resolutions beyond 4 Å for certain icosahedral viruses, enabling ab initio atomic model building of these viruses. As the only samples that achieved such high resolution at that time, cryo-EM method development was closely intertwined ...
Dongjie, Zhu +2 more
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