Results 161 to 170 of about 1,110,843 (197)

Cryoelectron microscopy of macromolecular complexes

Biology of the Cell, 1994
Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X‐ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies.
R H, Wade, E A, Hewat
openaire   +3 more sources

Cryoelectron microscopy of refrozen cryosections

Journal of Structural Biology, 2003
Cryoelectron microscopy makes it possible to record high-resolution detail from large and complex structures. However, its application to understanding cellular structure is limited by the requirement that samples should be no thicker than approximately 0.5-1 microm.
Pradeep K, Luther, Edward P, Morris
openaire   +2 more sources

Cryoelectron Microscopy of Fission Yeast

Cold Spring Harbor Protocols, 2017
Fission yeast cells can be prepared for electron microscopy (EM) in the frozen-hydrated state. This eliminates the requirement for dehydration and heavy metal staining when preparing samples for EM. As with room temperature imaging, however, the yeast must be sectioned to make them thin enough for transmission of the electron beam.
Mary K, Morphew, Thomas H, Giddings, J Richard, McIntosh   +2 more
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Cryoelectron Microscopy of Liposomes

2005
A thin aqueous film of suspended lipid vesicles?micelles is the object of choice for vitrification and subsequent study by cryoelectron microscopy. Just prior to vitrification, a thin film (compare with a soap film) is vulnerable to heat and mass exchange.
Peter M, Frederik, D H W, Hubert
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Cryoelectron microscopy of vitrified Sendai virions

Journal of Virological Methods, 1988
Morphology of vitrified Sendai virions was studied by transmission type electron microscopy. Almost all the virions appeared to be completely spherical, although their diameters differed. A possibly continuously long nucleocapsid was seen running helically in an envelope. Spikes were seen on the virion surfaces.
Y, Hosaka, T, Watabe
openaire   +2 more sources

Homology Modeling and Cryoelectron Microscopy

2018
Zachary F Burton
exaly   +2 more sources

Full-length, Oligomeric Structure of Wzz Determined by Cryoelectron Microscopy Reveals Insights into Membrane-Bound States

Structure, 2017
Wzz is an integral inner membrane protein involved in regulating the length of lipopolysaccharide O-antigen glycans and essential for the virulence of many Gram-negative pathogens.
Robert Ford, Daniel Clare, Chris Whitfield   +2 more
exaly   +2 more sources

Cryoelectron Microscopy of Icosahedral Virus Particles

2007
With the rapid progresses in both instrumentation and computing, it is increasingly straightforward and routine to determine the structures of icosahedral viruses to subnanometer resolutions (6-10 A) by cryoelectron microscopy and image reconstruction.
Wen, Jiang, Wah, Chiu
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Virus structures revealed by advanced cryoelectron microscopy methods

Structure, 2023
Before the resolution revolution, cryoelectron microscopy (cryo-EM) single-particle analysis (SPA) already achieved resolutions beyond 4 Å for certain icosahedral viruses, enabling ab initio atomic model building of these viruses. As the only samples that achieved such high resolution at that time, cryo-EM method development was closely intertwined ...
Dongjie, Zhu, Duanfang, Cao, Xinzheng, Zhang   +2 more
openaire   +2 more sources

Automated Identification of Filaments in Cryoelectron Microscopy Images

Journal of Structural Biology, 2001
Since the foundation for the three-dimensional image reconstruction of helical objects from electron micrographs was laid more than 30 years ago, there have been sustained developments in specimen preparation, data acquisition, image analysis, and interpretation of results.
Y, Zhu, B, Carragher, D J, Kriegman, R A, Milligan, C S, Potter   +4 more
openaire   +2 more sources