Results 161 to 170 of about 22,654 (207)

Cryoelectron Microscopy of Fission Yeast

Cold Spring Harbor Protocols, 2017
Fission yeast cells can be prepared for electron microscopy (EM) in the frozen-hydrated state. This eliminates the requirement for dehydration and heavy metal staining when preparing samples for EM. As with room temperature imaging, however, the yeast must be sectioned to make them thin enough for transmission of the electron beam.
Mary K, Morphew, Thomas H, Giddings, J Richard, McIntosh   +2 more
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Cryoelectron microscopy of refrozen cryosections

Journal of Structural Biology, 2003
Cryoelectron microscopy makes it possible to record high-resolution detail from large and complex structures. However, its application to understanding cellular structure is limited by the requirement that samples should be no thicker than approximately 0.5-1 microm.
Pradeep K, Luther, Edward P, Morris
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Cryoelectron microscopy of vitrified Sendai virions

Journal of Virological Methods, 1988
Morphology of vitrified Sendai virions was studied by transmission type electron microscopy. Almost all the virions appeared to be completely spherical, although their diameters differed. A possibly continuously long nucleocapsid was seen running helically in an envelope. Spikes were seen on the virion surfaces.
Y, Hosaka, T, Watabe
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Virus structures revealed by advanced cryoelectron microscopy methods

Structure, 2023
Before the resolution revolution, cryoelectron microscopy (cryo-EM) single-particle analysis (SPA) already achieved resolutions beyond 4 Å for certain icosahedral viruses, enabling ab initio atomic model building of these viruses. As the only samples that achieved such high resolution at that time, cryo-EM method development was closely intertwined ...
Dongjie, Zhu, Duanfang, Cao, Xinzheng, Zhang   +2 more
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Cryoelectron Microscopy of Liposomes

2005
A thin aqueous film of suspended lipid vesicles?micelles is the object of choice for vitrification and subsequent study by cryoelectron microscopy. Just prior to vitrification, a thin film (compare with a soap film) is vulnerable to heat and mass exchange.
Peter M, Frederik, D H W, Hubert
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Cryoelectron microscopy and cryoelectron tomography of the nuclear pre-mRNA processing machine

Journal of Structural Biology, 2002
Large nuclear ribonucleoprotein particles, which can be viewed as the naturally assembled precursor messenger RNA (pre-mRNA) processing machine, were analyzed in frozen-hydrated preparations by cryoelectron microscopy. A general and reproducible strategy for preparing ice-embedded large nuclear ribonucleoprotein (lnRNP) particles at sufficiently high ...
Medalia, O., Typke, D., Hegerl, R., Angenitzki, M., Sperling, J., Sperling, R.   +5 more
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Cryoelectron Microscopy of Icosahedral Virus Particles

2007
With the rapid progresses in both instrumentation and computing, it is increasingly straightforward and routine to determine the structures of icosahedral viruses to subnanometer resolutions (6-10 A) by cryoelectron microscopy and image reconstruction.
Wen, Jiang, Wah, Chiu
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Cryoelectron microscopy with elemental sensitivity

Structure
In a recent issue of Nature Methods, Pfeil-Gardiner et al. (2024)1 combine electron energy-loss spectroscopy and single-particle cryoelectron microscopy to allow the spatially resolved imaging of the elemental composition of macromolecules.
Hannah, Ochner, Tanmay A M, Bharat
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Cryoultramicrotomy: cryoelectron microscopy of vitreous sections.

Methods in molecular biology (Clifton, N.J.), 2007
Cryoultramicrotomy allows the sectioning of vitrified biological samples. These biological samples are preserved at the atomic level and represent the real structure at the moment of freezing. Cryoultramicrotomy produces ultra-thin cryosections that are investigated in a cryoelectron microscope.
Dimitri, Vanhecke, Luca, Studer, Daniel, Studer   +2 more
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Structure of bluetongue virus particles by cryoelectron microscopy

Journal of Structural Biology, 1992
The structure of the bluetongue virus (BTV) particle, determined by cryoelectron microscopy and image analysis, reveals a well-ordered outer shell which differs markedly from other known Reoviridae. The inner shell is known to have an icosahedral structure with 260 triangular spikes of VP7 trimers arranged on a T = 13,l lattice. The outer shell is seen
E A, Hewat, T F, Booth, P, Roy
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