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Recent Advances in Cryo Electron Microscopy and Tomography [PDF]

Structural Dynamics
In the recent years, cryo-electron microscopy (cryo-EM) single-particle analysis (SPA) has greatly benefited from a multitude of technological advances cumulating in breaking the atomic-resolution barrier for biological macromolecules.
Eliza Nieweglowska, Marc M. H. Storms, Matt Joens   +2 more
doaj   +5 more sources

Artificial Intelligence in Cryo-Electron Microscopy

Life, 2022
Cryo-electron microscopy (cryo-EM) has become an unrivaled tool for determining the structure of macromolecular complexes. The biological function of macromolecular complexes is inextricably tied to the flexibility of these complexes.
Jeong Min Chung, Clarissa L. Durie, Jinseok Lee   +2 more
doaj   +3 more sources

Cryo-electron microscopy-based drug design [PDF]

Frontiers in Molecular Biosciences
Structure-based drug design (SBDD) has gained popularity owing to its ability to develop more potent drugs compared to conventional drug-discovery methods.
Ecenur Cebi, Joohyun Lee, Vinod Kumar Subramani, Nayeon Bak, Changsuk Oh, Kyeong Kyu Kim   +5 more
doaj   +2 more sources

Assembly mechanism of the pleomorphic immature poxvirus scaffold

Nature Communications, 2022
Immature poxviruses are characterized by nonicosahedral semiordered protein scaffolds critical for morphogenesis. Here, the authors use cryo-EM structures of Vaccinia virus D13 scaffold intermediates to explain their assembly mechanism.
Jaekyung Hyun, Hideyuki Matsunami, Tae Gyun Kim, Matthias Wolf   +3 more
doaj   +1 more source

Cryo-electron microscopy structures of capsids and in situ portals of DNA-devoid capsids of human cytomegalovirus

Nature Communications, 2023
The portal-scaffold complex is believed to nucleate the assembly of herpesvirus procapsids. During capsid maturation, two events occur: scaffold expulsion and DNA incorporation. The portal-scaffold interaction and the conformational changes that occur to
Zhihai Li, Jingjing Pang, Rongchao Gao, Qingxia Wang, Maoyan Zhang, Xuekui Yu   +5 more
doaj   +1 more source

Cohomology of Cryo-Electron Microscopy [PDF]

SIAM Journal on Applied Algebra and Geometry, 2017
The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this article, we show that the basic premise of cryo-EM --- patching together 2-dimensional projections to reconstruct a 3-dimensional object --- is naturally one of Cech cohomology with SO(2)-
Ke Ye, Lek-Heng Lim
openaire   +2 more sources

Structural insights into RNA bridging between HIV-1 Vif and antiviral factor APOBEC3G

Nature Communications, 2023
Great effort has been devoted to discovering the basis of A3G-Vif interaction, the key event of HIV’s counteraction mechanism to evade antiviral innate immune response.
Takahide Kouno, Satoshi Shibata, Megumi Shigematsu, Jaekyung Hyun, Tae Gyun Kim, Hiroshi Matsuo, Matthias Wolf   +6 more
doaj   +1 more source

COVID-19 serological survey using micro blood sampling

Scientific Reports, 2021
During August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of ...
Melissa M. Matthews, Tae Gyun Kim, Satoshi Shibata, Noriko Shibata, Christian Butcher, Jaekyung Hyun, Keon Young Kim, Theodore Robb, Siang Sheng Jheng, Masashi Narita, Tomoari Mori, Mary Collins, Matthias Wolf   +12 more
doaj   +1 more source

Temporal dynamics of charge buildup in cryo-electron microscopy

Journal of Structural Biology: X, 2023
It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly understood.
Makoto Tokoro Schreiber, Alan Maigné, Marco Beleggia, Satoshi Shibata, Matthias Wolf   +4 more
doaj   +1 more source

Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy

Cell Discovery, 2022
Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals. Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical ...
Aojie Wang, Feng Zhou, Congcong Liu, Dongsheng Gao, Ruxi Qi, Yiheng Yin, Sheng Liu, Yuanzhu Gao, Lutang Fu, Yinhe Xia, Yawei Xu, Chuanqing Wang, Zheng Liu   +12 more
doaj   +1 more source