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Biological cryo‐electron microscopy in China [PDF]
Protein Science, 2016 AbstractCryo‐electron microscopy (cryo‐EM) plays an increasingly more important role in structural biology. With the construction of an arm of the Chinese National Protein Science Facility at Tsinghua University, biological cryo‐EM has entered a phase of rapid development in China. This article briefly reviews the history of biological cryo‐EM in China,
Hong‐Wei Wang, Jianlin Lei, Yigong Shi
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Artificial Intelligence in Cryo-Electron Microscopy
Life, 2022 Cryo-electron microscopy (cryo-EM) has become an unrivaled tool for determining the structure of macromolecular complexes. The biological function of macromolecular complexes is inextricably tied to the flexibility of these complexes. Single particle cryo-EM can reveal the conformational heterogeneity of a biochemically pure sample, leading to well ...
Jeong Chung, Clarissa Durie, Jinseok Lee
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Cryo-electron microscopy-based drug design
Frontiers in Molecular BiosciencesStructure-based drug design (SBDD) has gained popularity owing to its ability to develop more potent drugs compared to conventional drug-discovery methods. The success of SBDD relies heavily on obtaining the three-dimensional structures of drug targets. X-ray crystallography is the primary method used for solving structures and aiding the SBDD workflow;
Ecenur Cebi +5 more
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Assembly mechanism of the pleomorphic immature poxvirus scaffold
Nature Communications, 2022 Immature poxviruses are characterized by nonicosahedral semiordered protein scaffolds critical for morphogenesis. Here, the authors use cryo-EM structures of Vaccinia virus D13 scaffold intermediates to explain their assembly mechanism.
Jaekyung Hyun +3 more
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Cohomology of Cryo-Electron Microscopy [PDF]
SIAM Journal on Applied Algebra and Geometry, 2017 The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this article, we show that the basic premise of cryo-EM --- patching together 2-dimensional projections to reconstruct a 3-dimensional object --- is naturally one of Cech cohomology with SO(2)-
Ke Ye, Lek-Heng Lim
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Nature Communications, 2023 The portal-scaffold complex is believed to nucleate the assembly of herpesvirus procapsids. During capsid maturation, two events occur: scaffold expulsion and DNA incorporation. The portal-scaffold interaction and the conformational changes that occur to
Zhihai Li +5 more
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Structural insights into RNA bridging between HIV-1 Vif and antiviral factor APOBEC3G
Nature Communications, 2023 Great effort has been devoted to discovering the basis of A3G-Vif interaction, the key event of HIV’s counteraction mechanism to evade antiviral innate immune response.
Takahide Kouno +6 more
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COVID-19 serological survey using micro blood sampling
Scientific Reports, 2021 During August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of ...
Melissa M. Matthews +12 more
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Temporal dynamics of charge buildup in cryo-electron microscopy
Journal of Structural Biology: X, 2023 It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly understood.
Makoto Tokoro Schreiber +4 more
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Cryo-electron microscopy of viruses [PDF]
Nature, 1984 Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional
Adrian, Marc +3 more
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