Results 11 to 20 of about 68,000 (256)
Cryo-electron microscopy of viruses [PDF]
Nature, 1984 Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional
Adrian, Marc +3 more
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Structural basis for genome packaging, retention, and ejection in human cytomegalovirus
Nature Communications, 2021 Human cytomegalovirus (HCMV) is the prototypical member of the β-herpesvirinae subfamily and the leading viral cause of congenital infections that can lead to birth defects and it can also cause life-threatening disease in immunocompromised individuals ...
Zhihai Li +3 more
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Nature Communications, 2023 The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral
Rafael Ayala +9 more
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Biological cryo‐electron microscopy in China [PDF]
Protein Science, 2016 AbstractCryo‐electron microscopy (cryo‐EM) plays an increasingly more important role in structural biology. With the construction of an arm of the Chinese National Protein Science Facility at Tsinghua University, biological cryo‐EM has entered a phase of rapid development in China. This article briefly reviews the history of biological cryo‐EM in China,
Hong‐Wei Wang, Jianlin Lei, Yigong Shi
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Measuring the effects of ice thickness on resolution in single particle cryo-EM
Journal of Structural Biology: X, 2023 Ice thickness is a critical parameter in single particle cryo-EM – too thin ice can break during imaging or exclude the sample of interest, while ice that is too thick contributes to more inelastic scattering that precludes obtaining high resolution ...
Kasahun Neselu +5 more
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Cryo‐electron microscopy of vitreous sections [PDF]
The EMBO Journal, 2004 Since the beginning of the 1980s, cryo-electron microscopy of a thin film of vitrified aqueous suspension has made it possible to observe biological particles in their native state, in the absence of the usual artefacts of dehydration and staining.
Al-Amoudi, Ashraf +9 more
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Best practice: setting up and operating a mid-sized cryo-EM facility
Frontiers in Molecular Biosciences, 2023 Ever since the resolution revolution in 2013, cryo-electron microscopy (cryo-EM) has become a powerful methodology in structural biology that is especially suited to study the structure of large flexible molecular complexes.
Xing Meng +8 more
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4D Cryo-Electron Microscopy of Proteins [PDF]
Journal of the American Chemical Society, 2013 Cryo-electron microscopy is a form of transmission electron microscopy that has been used to determine the 3D structure of biological specimens in the hydrated state and with high resolution. We report the development of 4D cryo-electron microscopy by integrating the fourth dimension, time, into this powerful technique.
Fitzpatrick, AWP +3 more
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MATHEMATICS FOR CRYO-ELECTRON MICROSCOPY [PDF]
Proceedings of the International Congress of Mathematicians (ICM 2018), 2019 Proceedings of the International Congress of Mathematicians ...
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Cryo-electron Microscopy of Membrane Proteins [PDF]
, 2013 Electron crystallography is used to study membrane proteins in the form of planar, two-dimensional (2D) crystals, or other crystalline arrays such as tubular crystals. This method has been used to determine the atomic resolution structures of bacteriorhodopsin, tubulin, aquaporins, and several other membrane proteins.
Goldie, Kenneth N +5 more
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