Results 41 to 50 of about 133,701 (285)
Cryo-electron microscopy shapes up [PDF]
Nature, 2018 As the imaging technique produces ever-sharper protein structures, researchers are racing to develop tools to assess how accurate they are. As the imaging technique produces ever-sharper protein structures, researchers are racing to develop tools to assess how accurate they are.
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Cryo-electron microscopy of chromatin biology [PDF]
Acta Crystallographica Section D Structural Biology, 2017 The basic unit of chromatin, the nucleosome core particle (NCP), controls how DNA in eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have sought to understand how this protein–DNA complex can help to control so many diverse tasks.
Marcus D. Wilson, Alessandro Costa
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Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion. [PDF]
, 2012 The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle-vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Brunger, Axel T +13 more
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Testing of therapies in a novel nebulin nemaline myopathy model demonstrate a lack of efficacy
Acta Neuropathologica Communications, 2018 Nemaline myopathies are heterogeneous congenital muscle disorders causing skeletal muscle weakness and, in some cases, death soon after birth. Mutations in nebulin, encoding a large sarcomeric protein required for thin filament function, are responsible ...
Tamar E. Sztal +5 more
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Microstructural characterization of a Canadian oil sand [PDF]
, 2012 The microstructure of oil sand samples extracted at a depth of 75 m from the estuarine Middle McMurray formation (Alberta, Canada) has been investigated by using high resolution 3D X-Ray microtomography ($\mu$CT) and Cryo Scanning Electron Microscopy ...
Delage, Pierre +4 more
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Switchable resolution in soft x-ray tomography of single cells. [PDF]
, 2020 The diversity of living cells, in both size and internal complexity, calls for imaging methods with adaptable spatial resolution. Soft x-ray tomography (SXT) is a three-dimensional imaging technique ideally suited to visualizing and quantifying the ...
Chao, Weilun +10 more
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Cryo-electron Microscopy of Membrane Proteins [PDF]
, 2013 Electron crystallography is used to study membrane proteins in the form of planar, two-dimensional (2D) crystals, or other crystalline arrays such as tubular crystals. This method has been used to determine the atomic resolution structures of bacteriorhodopsin, tubulin, aquaporins, and several other membrane proteins.
Goldie, Kenneth N. +5 more
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Cryo-electron microscopy of membrane proteins [PDF]
Methods, 2018 Membrane proteins represent a large proportion of the proteome, but have characteristics that are problematic for many methods in modern molecular biology (that have often been developed with soluble proteins in mind). For structural studies, low levels of expression and the presence of detergent have been thorns in the flesh of the membrane protein ...
Thonghin, Nopnithi +3 more
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Atomic Resolution Structure of the Oncolytic Parvovirus LuIII by Electron Microscopy and 3D Image Reconstruction. [PDF]
, 2017 LuIII, a protoparvovirus pathogenic to rodents, replicates in human mitotic cells, making it applicable for use to kill cancer cells. This virus group includes H-1 parvovirus (H-1PV) and minute virus of mice (MVM).
Agbandje-McKenna, Mavis +13 more
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Cryo‐electron microscopy of vitreous sections [PDF]
The EMBO Journal, 2004 Since the beginning of the 1980s, cryo-electron microscopy of a thin film of vitrified aqueous suspension has made it possible to observe biological particles in their native state, in the absence of the usual artefacts of dehydration and staining.
Al-Amoudi, Ashraf +9 more
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