Results 41 to 50 of about 2,439,094 (286)
`Cryo-EM': electron cryomicroscopy, cryo electron microscopy or something else?
IUCrJ, 2023 Structural biology continues to benefit from an expanding toolkit, which is helping to gain unprecedented insight into the assembly and organization of multi-protein machineries, enzyme mechanisms and ligand/inhibitor binding.
Richard Henderson, Samar Hasnain
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Structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators
Nature Communications, 2022 The Kv1.3 potassium channel is expressed abundantly on activated T cells and mediates the cellular immune responses. Here, the authors report structures of the Kv1.3 potassium channel with and without immunoglobulin modulators, shedding light on the ...
Purushotham Selvakumar +10 more
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Strain engineering in alloy nanoparticles
Advances in Physics: X, 2023 The deformation of interatomic distances with respect to those of the perfect crystal generates atomic-level strain. In nanoalloys, strain can arise because of finite size, morphology, domain structure and lattice mismatch between their atomic compounds.
Diana Nelli +2 more
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Cryo-electron microscopy of membrane proteins [PDF]
Methods, 2018 Membrane proteins represent a large proportion of the proteome, but have characteristics that are problematic for many methods in modern molecular biology (that have often been developed with soluble proteins in mind). For structural studies, low levels of expression and the presence of detergent have been thorns in the flesh of the membrane protein ...
Nopnithi, Thonghin +3 more
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Cryo-electron microscopy of the chromatin fiber
Current Opinion in Structural Biology, 2020 The three-dimensional (3D) organization of chromatin plays a crucial role in the regulation of gene expression. Chromatin conformation is strongly affected by the composition, structural features and dynamic properties of the nucleosome, which in turn determine the nature and geometry of interactions that can occur between neighboring nucleosomes ...
Boopathi, Ramachandran +4 more
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Cryo-electron microscopy of the giant viruses
Microscopy, 2021 Abstract High-resolution study of the giant viruses presents one of the latest challenges in cryo-electron microscopy (EM) of viruses. Too small for light microscopy but too large for easy study at high resolution by EM, they range in size from ∼0.2 to 2 μm from high-symmetry icosahedral viruses, such as Paramecium burseria Chlorella ...
Raymond N, Burton-Smith +1 more
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Imaging and 3D reconstruction of membrane protein complexes by cryo-electron microscopy and single particle analysis [PDF]
, 2006 Cryo-electron microscopy (cryo-EM) in combination with single particle image processing and volume reconstruction is a powerful technology to obtain medium-resolution structures of large protein complexes, which are extremely difficult to crystallize and ...
Gregorini, Marco
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Testing of therapies in a novel nebulin nemaline myopathy model demonstrate a lack of efficacy
Acta Neuropathologica Communications, 2018 Nemaline myopathies are heterogeneous congenital muscle disorders causing skeletal muscle weakness and, in some cases, death soon after birth. Mutations in nebulin, encoding a large sarcomeric protein required for thin filament function, are responsible ...
Tamar E. Sztal +5 more
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Cryo-electron microscopy of chromatin biology [PDF]
Acta Crystallographica Section D Structural Biology, 2017 The basic unit of chromatin, the nucleosome core particle (NCP), controls how DNA in eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have sought to understand how this protein–DNA complex can help to control so many diverse tasks.
Marcus D. Wilson, Alessandro Costa
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RIBFIND: a web server for identifying rigid bodies in protein structures and to aid flexible fitting into cryo EM maps [PDF]
, 2012 Motivation: To better analyze low-resolution cryo electron microscopy maps of macromolecular assemblies, component atomic structures frequently have to be flexibly fitted into them.
Topf, Maya +3 more
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