Results 41 to 50 of about 68,000 (256)

Thermoresponsive Hydrogels with Improved Actuation Function by Interconnected Microchannels

Advanced Intelligent Systems, 2022
Stimuli−responsive hydrogels are important in soft actuators research, as they change volume in response to environmental factors. Thermoresponsive hydrogels, such as poly(N‐isopropylacrylamide) (pNIPAM), typically have slow response rates and exert ...
Tobias Spratte, Christine Arndt, Irene Wacker, Margarethe Hauck, Rainer Adelung, Rasmus R. Schröder, Fabian Schütt, Christine Selhuber-Unkel   +7 more
doaj   +1 more source

Pharmacological hallmarks of allostery at the M4 muscarinic receptor elucidated through structure and dynamics

eLife, 2023
Allosteric modulation of G protein-coupled receptors (GPCRs) is a major paradigm in drug discovery. Despite decades of research, a molecular-level understanding of the general principles that govern the myriad pharmacological effects exerted by GPCR ...
Ziva Vuckovic, Jinan Wang, Vi Pham, Jesse I Mobbs, Matthew J Belousoff, Apurba Bhattarai, Wessel AC Burger, Geoff Thompson, Mahmuda Yeasmin, Vindhya Nawaratne, Katie Leach, Emma T van der Westhuizen, Elham Khajehali, Yi-Lynn Liang, Alisa Glukhova, Denise Wootten, Craig W Lindsley, Andrew Tobin, Patrick Sexton, Radostin Danev, Celine Valant, Yinglong Miao, Arthur Christopoulos, David M Thal   +23 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Cryo‐electron microscopy of vitrified chromosomes in situ. [PDF]

The EMBO Journal, 1986
Chromosomes of metaphase-arrested Chinese hamster ovary (CHO) and HeLa cells were examined in situ, unfixed and unstained, by cryo-electron microscopy. In hydrated, vitrified cryo-sections, chromosomes exhibit a characteristic homogeneous, grainy texture, which, on optical diffraction, gives rise to a broad reflection corresponding to 11 nm.
McDowall, A.W., Smith, J.M., Dubochet, J.   +2 more
openaire   +5 more sources

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Engineered protein subunit COVID19 vaccine is as immunogenic as nanoparticles in mouse and hamster models

Scientific Reports
Initial studies on the immunogenicity of SARS-CoV-2 (CoV-2) S glycoprotein (“spike”) as a protein subunit vaccine suggested sub-optimal efficacy in mammals.
Melissa M. Matthews, Tae Gyun Kim, Keon Young Kim, Vladimir Meshcheryakov, Higor Alves Iha, Miho Tamai, Daiki Sasaki, Paola Laurino, Saacnicteh Toledo-Patiño, Mary Collins, Tzung-Yang Hsieh, Satoshi Shibata, Noriko Shibata, Fumiko Obata, Jun Fujii, Toshihiro Ito, Hiroshi Ito, Hiroki Ishikawa, Matthias Wolf   +18 more
doaj   +1 more source

Cryo-electron microscopy shapes up [PDF]

Nature, 2018
As the imaging technique produces ever-sharper protein structures, researchers are racing to develop tools to assess how accurate they are. As the imaging technique produces ever-sharper protein structures, researchers are racing to develop tools to assess how accurate they are.
openaire   +2 more sources

Cryo-Electron Microscopy for Cancer

International Journal of Trends in OncoScience, 2023
A review paper on cryo-electron microscopy (cryo-EM) is essential to assess the recent advancements in this revolutionary imaging technique. As cryo-EM continues to revolutionize structural biology, a comprehensive review can consolidate the knowledge, highlight technical challenges, and offer insights into future developments, promoting better ...
Dr Bablee Jyoti, Dr Anand Mohan Jha, Dr Jagadeesh Dhamodharan, Dr Aswinprakash Subramanian   +3 more
openaire   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source