Results 31 to 40 of about 68,000 (256)

Cryo-electron microscopy of chromatin biology [PDF]

Acta Crystallographica Section D Structural Biology, 2017
The basic unit of chromatin, the nucleosome core particle (NCP), controls how DNA in eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have sought to understand how this protein–DNA complex can help to control so many diverse tasks.
Marcus D. Wilson, Alessandro Costa
openaire   +3 more sources

Cryo-electron microscopy of the giant viruses

Microscopy, 2021
Abstract High-resolution study of the giant viruses presents one of the latest challenges in cryo-electron microscopy (EM) of viruses. Too small for light microscopy but too large for easy study at high resolution by EM, they range in size from ∼0.2 to 2 μm from high-symmetry icosahedral viruses, such as Paramecium burseria Chlorella ...
Raymond N, Burton-Smith, Kazuyoshi, Murata   +1 more
openaire   +2 more sources

Cryo-electron microscopy of membrane proteins [PDF]

Methods, 2018
Membrane proteins represent a large proportion of the proteome, but have characteristics that are problematic for many methods in modern molecular biology (that have often been developed with soluble proteins in mind). For structural studies, low levels of expression and the presence of detergent have been thorns in the flesh of the membrane protein ...
Nopnithi, Thonghin, Vasileios, Kargas, Jack, Clews, Robert C, Ford   +3 more
openaire   +2 more sources

A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization

Biomolecules, 2023
Viral entry and fertilization are distinct biological processes that share a common mechanism: membrane fusion. In viral entry, enveloped viruses attach to the host cell membrane, triggering a series of conformational changes in the viral fusion proteins.
Farshad C. Azimi, Trevor T. Dean, Karine Minari, Luis G. M. Basso, Tyler D. R. Vance, Vitor Hugo B. Serrão   +5 more
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

FEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile, Riccardo Diamanti, Daniel Lundin, Soni Sharma, Robin Lissner, Coenraad P. Kuil, Martin Högbom, Dirk Bald, Dan Sjöstrand   +8 more
wiley   +1 more source

Structural Insights into Cell Wall-Related Vesicle Secretion with Different Mechanisms

Plants
Exocytosis is a fundamental biological process in all eukaryotes involving the vesicular transport of cellular cargo to the plasma membrane or extracellular space.
Jiawen Yang, Sheng Chang, Linlin Li, Xiaoyu Ma, Hua Han, Zheng Liu, Jian Zhu   +6 more
doaj   +1 more source

Orthogonal matrix retrieval in cryo-electron microscopy [PDF]

2015 IEEE 12th International Symposium on Biomedical Imaging (ISBI), 2015
In single particle reconstruction (SPR) from cryo-electron microscopy (cryo-EM), the 3D structure of a molecule needs to be determined from its 2D projection images taken at unknown viewing directions. Zvi Kam showed already in 1980 that the autocorrelation function of the 3D molecule over the rotation group SO(3) can be estimated from 2D projection ...
Tejal Bhamre, Teng Zhang 0002, Amit Singer   +2 more
openaire   +3 more sources

Identification of the plant mitochondrial OrfX protein: A mass spectrometry approach

FEBS Letters, EarlyView.
The mitochondrial genome of plants contains an open reading frame, orfx, which encodes a rare protein that has so far escaped mass spectrometric detection. The protein resembles the c‐subunit of bacterial twin‐arginine‐motif‐dependent protein translocases (TatC).
Matthias Döring, Jan de Jonge, Hans‐Peter Braun, Nils Rugen   +3 more
wiley   +1 more source

The RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes

Nature Communications, 2016
Continuous cycles of nucleo-cytoplasmic transport require disassembly of transport receptor-Ran-GTP complexes in the cytoplasm. Here the authors elucidate the specific function of the RanBP2 complex in the disassembly process.
Tobias Ritterhoff, Hrishikesh Das, Götz Hofhaus, Rasmus R. Schröder, Annette Flotho, Frauke Melchior   +5 more
doaj   +1 more source