Results 31 to 40 of about 6,053 (258)
Cryo-electron microscopy of membrane proteins [PDF]
Methods, 2018 Membrane proteins represent a large proportion of the proteome, but have characteristics that are problematic for many methods in modern molecular biology (that have often been developed with soluble proteins in mind). For structural studies, low levels of expression and the presence of detergent have been thorns in the flesh of the membrane protein ...
Nopnithi, Thonghin +3 more
openaire +2 more sources
A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization
Biomolecules, 2023 Viral entry and fertilization are distinct biological processes that share a common mechanism: membrane fusion. In viral entry, enveloped viruses attach to the host cell membrane, triggering a series of conformational changes in the viral fusion proteins.
Farshad C. Azimi +5 more
doaj +1 more source
Cryo-electron microscopy of the chromatin fiber
Current Opinion in Structural Biology, 2020 The three-dimensional (3D) organization of chromatin plays a crucial role in the regulation of gene expression. Chromatin conformation is strongly affected by the composition, structural features and dynamic properties of the nucleosome, which in turn determine the nature and geometry of interactions that can occur between neighboring nucleosomes ...
Boopathi, Ramachandran +4 more
openaire +5 more sources
pH‐mediated activation of the lysosomal arginine sensor SLC38A9
FEBS Letters, EarlyView.Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley +1 more source
Cryo-electron microscopy shapes up [PDF]
Nature, 2018 As the imaging technique produces ever-sharper protein structures, researchers are racing to develop tools to assess how accurate they are. As the imaging technique produces ever-sharper protein structures, researchers are racing to develop tools to assess how accurate they are.
openaire +2 more sources
Thermoresponsive Hydrogels with Improved Actuation Function by Interconnected Microchannels
Advanced Intelligent Systems, 2022 Stimuli−responsive hydrogels are important in soft actuators research, as they change volume in response to environmental factors. Thermoresponsive hydrogels, such as poly(N‐isopropylacrylamide) (pNIPAM), typically have slow response rates and exert ...
Tobias Spratte +7 more
doaj +1 more source
Biophysical approaches for studying viral entry
FEBS Letters, EarlyView.Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley +1 more source
Orthogonal matrix retrieval in cryo-electron microscopy [PDF]
2015 IEEE 12th International Symposium on Biomedical Imaging (ISBI), 2015 In single particle reconstruction (SPR) from cryo-electron microscopy (cryo-EM), the 3D structure of a molecule needs to be determined from its 2D projection images taken at unknown viewing directions. Zvi Kam showed already in 1980 that the autocorrelation function of the 3D molecule over the rotation group SO(3) can be estimated from 2D projection ...
Tejal Bhamre +2 more
openaire +3 more sources
Structural Insights into Cell Wall-Related Vesicle Secretion with Different Mechanisms
PlantsExocytosis is a fundamental biological process in all eukaryotes involving the vesicular transport of cellular cargo to the plasma membrane or extracellular space.
Jiawen Yang +6 more
doaj +1 more source
Nature Communications, 2016 Continuous cycles of nucleo-cytoplasmic transport require disassembly of transport receptor-Ran-GTP complexes in the cytoplasm. Here the authors elucidate the specific function of the RanBP2 complex in the disassembly process.
Tobias Ritterhoff +5 more
doaj +1 more source