Results 31 to 40 of about 2,439,094 (286)
Biological cryo‐electron microscopy in China [PDF]
Protein Science, 2016 AbstractCryo‐electron microscopy (cryo‐EM) plays an increasingly more important role in structural biology. With the construction of an arm of the Chinese National Protein Science Facility at Tsinghua University, biological cryo‐EM has entered a phase of rapid development in China. This article briefly reviews the history of biological cryo‐EM in China,
Hong‐Wei Wang, Jianlin Lei, Yigong Shi
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Measuring the effects of ice thickness on resolution in single particle cryo-EM
Journal of Structural Biology: X, 2023 Ice thickness is a critical parameter in single particle cryo-EM – too thin ice can break during imaging or exclude the sample of interest, while ice that is too thick contributes to more inelastic scattering that precludes obtaining high resolution ...
Kasahun Neselu +5 more
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Adapting cryogenic correlative light and electron microscopy (cryo-CLEM) for food oxidation studies [PDF]
, 2023 Lipid oxidation is a major cause of product deterioration in protein stabilised oil-in-water food emulsions. The impact of protein emulsifiers on lipid oxidation and the stability depends on the specific type of protein emulsifiers used and the redox ...
Johannes, Hohlbein +5 more
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Cryo‐electron microscopy of vitreous sections [PDF]
The EMBO Journal, 2004 Since the beginning of the 1980s, cryo-electron microscopy of a thin film of vitrified aqueous suspension has made it possible to observe biological particles in their native state, in the absence of the usual artefacts of dehydration and staining.
Al-Amoudi, Ashraf +9 more
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Best practice: setting up and operating a mid-sized cryo-EM facility
Frontiers in Molecular Biosciences, 2023 Ever since the resolution revolution in 2013, cryo-electron microscopy (cryo-EM) has become a powerful methodology in structural biology that is especially suited to study the structure of large flexible molecular complexes.
Xing Meng +8 more
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High-throughput high-resolution cryo-electron crystallography [PDF]
, 2015 High-resolution structures of membrane and soluble proteins can be obtained by cryo-electron crystallography, given highly-ordered cryo-preparations of perfectly flat 2D crystals are available.
Scherer, Sebastian
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MATHEMATICS FOR CRYO-ELECTRON MICROSCOPY [PDF]
Proceedings of the International Congress of Mathematicians (ICM 2018), 2019 Proceedings of the International Congress of Mathematicians ...
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CRYO-ELECTRON MICROSCOPY OF SPLICEOSOMAL COMPONENTS
Annual Review of Biophysics and Biomolecular Structure, 2006 Splicing is an essential step of gene expression in which introns are removed from pre-mRNA to generate mature mRNA that can be translated by the ribosome. This reaction is catalyzed by a large and dynamic macromolecular RNP complex called the spliceosome. The spliceosome is formed by the stepwise integration of five snRNPs composed of U1, U2, U4, U5,
Stark, Holger, Lührmann, Reinhard
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Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes
Cell Reports, 2021 Summary: The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism, and appetite and is an important target for treatment of type 2 diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials,
Xin Zhang +5 more
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The cryo-electron microscopy structure of huntingtin [PDF]
Nature, 2018 Huntingtin (HTT) is a large (348 kDa) protein that is essential for embryonic development and is involved in diverse cellular activities such as vesicular transport, endocytosis, autophagy and the regulation of transcription. Although an integrative understanding of the biological functions of HTT is lacking, the large number of identified HTT ...
Guo, Q. +13 more
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