Developing Graphene Grids for Cryoelectron Microscopy [PDF]
Frontiers in Molecular Biosciences, 2022 Cryogenic electron microscopy (cryo-EM) single particle analysis has become one of the major techniques used to study high-resolution 3D structures of biological macromolecules.
Hongcheng Fan +5 more
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Purification and cryoelectron microscopy structure determination of human V-ATPase [PDF]
STAR Protocols, 2021 Summary: Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are multi-component, ATP-driven proton pumps, which play important roles in many physiological processes by acidifying intracellular vesicles, organelles, and the extracellular ...
Longfei Wang +3 more
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Determining structures in a native environment using single-particle cryoelectron microscopy images [PDF]
The Innovation, 2021 Cryo-electron tomography is a powerful tool for structure determination in the native environment. However, this method requires the acquisition of tilt series, which is time-consuming and severely slows structure determination. By treating the densities
Jing Cheng +3 more
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Cryoelectron microscopy structures of a human neutralizing antibody bound to MERS-CoV spike glycoprotein [PDF]
Frontiers in Microbiology, 2022 Neutralizing monoclonal antibodies (mAbs) against highly pathogenic coronaviruses represent promising candidates for clinical intervention. Here, we isolated a potent neutralizing monoclonal antibody, MERS-S41, from a yeast displayed scFv library using ...
Shuyuan Zhang +10 more
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Cryoelectron Microscopy Structures of AdeB Illuminate Mechanisms of Simultaneous Binding and Exporting of Substrates [PDF]
mBio, 2021 Acinetobacter ...
Christopher E. Morgan +7 more
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Macromolecular refinement of X-ray and cryoelectron microscopy structures with Phenix/OPLS3e for improved structure and ligand quality [PDF]
Structure, 2021 Gydo C P Van Zundert +2 more
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High-resolution single-particle cryo-EM of samples vitrified in boiling nitrogen
IUCrJ, 2021 Based on work by Dubochet and others in the 1980s and 1990s, samples for single-particle cryo-electron microscopy (cryo-EM) have been vitrified using ethane, propane or ethane/propane mixtures.
Tyler Engstrom +7 more
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Antibody-Based Affinity Cryoelectron Microscopy at 2.6-Å Resolution [PDF]
Structure, 2016 Guimei Yu, KunPeng Li, Pengwei Huang
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Artificial Intelligence in Cryo-Electron Microscopy
Life, 2022 Cryo-electron microscopy (cryo-EM) has become an unrivaled tool for determining the structure of macromolecular complexes. The biological function of macromolecular complexes is inextricably tied to the flexibility of these complexes.
Jeong Min Chung +2 more
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Mechanistic understanding of human SLFN11
Nature Communications, 2022 Schlafen 11 serves as an antiviral restriction factor and a predictive biomarker in cancer. Here, the authors use cryoelectron microscopy and biochemical assays to understand tRNA endoribonuclease and DNA binding functions of human Schlafen 11.
Felix J. Metzner +5 more
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