Developing Graphene Grids for Cryoelectron Microscopy [PDF]
Frontiers in Molecular Biosciences, 2022 Cryogenic electron microscopy (cryo-EM) single particle analysis has become one of the major techniques used to study high-resolution 3D structures of biological macromolecules.
Hongcheng Fan +5 more
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Purification and cryoelectron microscopy structure determination of human V-ATPase [PDF]
STAR Protocols, 2021 Summary: Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are multi-component, ATP-driven proton pumps, which play important roles in many physiological processes by acidifying intracellular vesicles, organelles, and the extracellular ...
Longfei Wang +3 more
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Determining structures in a native environment using single-particle cryoelectron microscopy images [PDF]
The Innovation, 2021 Cryo-electron tomography is a powerful tool for structure determination in the native environment. However, this method requires the acquisition of tilt series, which is time-consuming and severely slows structure determination. By treating the densities
Jing Cheng +3 more
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Cryoelectron microscopy structures of a human neutralizing antibody bound to MERS-CoV spike glycoprotein [PDF]
Frontiers in Microbiology, 2022 Neutralizing monoclonal antibodies (mAbs) against highly pathogenic coronaviruses represent promising candidates for clinical intervention. Here, we isolated a potent neutralizing monoclonal antibody, MERS-S41, from a yeast displayed scFv library using ...
Shuyuan Zhang +10 more
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Residue-level error detection in cryoelectron microscopy models. [PDF]
Structure, 2023 Building accurate protein models into moderate resolution (3-5 Å) cryoelectron microscopy (cryo-EM) maps is challenging and error prone. We have developed MEDIC (Model Error Detection in Cryo-EM), a robust statistical model that identifies local backbone errors in protein structures built into cryo-EM maps by combining local fit-to-density with deep ...
Reggiano G +4 more
europepmc +4 more sources
Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy. [PDF]
J Vis Exp, 2023 Cryogenic electron microscopy (cryoEM) has emerged as a powerful technique for probing the atomic structure of macromolecular complexes. Sample preparation for cryoEM requires preserving specimens in a thin layer of vitreous ice, typically suspended within the holes of a fenestrated support film. However, all commonly used sample preparation approaches
Basanta B, Chen W, Pride DE, Lander GC.
europepmc +3 more sources
Contributions of single-particle cryoelectron microscopy toward fighting COVID-19. [PDF]
Trends Biochem Sci, 2022 Single-particle cryoelectron microscopy (cryo-EM), whose full capabilities have been realized only within the past decade, has had a pivotal role in the fight against COVID-19. This is due to the technique's intrinsic power to depict both structural and dynamic features of molecules; in this case, of the spike protein of SARS-CoV-2.
Rapp M, Shapiro L, Frank J.
europepmc +4 more sources
Cryoelectron Microscopy Structures of AdeB Illuminate Mechanisms of Simultaneous Binding and Exporting of Substrates [PDF]
mBio, 2021 Acinetobacter ...
Christopher E. Morgan +7 more
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Structure of the AAVhu.37 capsid by cryoelectron microscopy. [PDF]
Acta Crystallogr F Struct Biol Commun, 2020 Adeno-associated viruses (AAVs) are used as in vivo gene-delivery vectors in gene-therapy products and have been heavily investigated for numerous indications. Over 100 naturally occurring AAV serotypes and variants have been isolated from primate samples.
Kaelber JT +6 more
europepmc +4 more sources
CryoSeek: A strategy for bioentity discovery using cryoelectron microscopy. [PDF]
Proc Natl Acad Sci U S AStructural biology is experiencing a paradigm shift from targeted structural determination to structure-guided discovery of previously uncharacterized bioentities. We employed cryoelectron microscopy (cryo-EM) to analyze filtered water samples collected from the Tsinghua Lotus Pond.
Wang T +6 more
europepmc +3 more sources