Developing Graphene Grids for Cryoelectron Microscopy [PDF]
Frontiers in Molecular Biosciences, 2022 Cryogenic electron microscopy (cryo-EM) single particle analysis has become one of the major techniques used to study high-resolution 3D structures of biological macromolecules.
Hongcheng Fan +5 more
doaj +2 more sources
Purification and cryoelectron microscopy structure determination of human V-ATPase [PDF]
STAR Protocols, 2021 Summary: Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are multi-component, ATP-driven proton pumps, which play important roles in many physiological processes by acidifying intracellular vesicles, organelles, and the extracellular ...
Longfei Wang +3 more
doaj +2 more sources
Determining structures in a native environment using single-particle cryoelectron microscopy images [PDF]
The Innovation, 2021 Cryo-electron tomography is a powerful tool for structure determination in the native environment. However, this method requires the acquisition of tilt series, which is time-consuming and severely slows structure determination. By treating the densities
Jing Cheng +3 more
doaj +2 more sources
Cryoelectron microscopy structures of a human neutralizing antibody bound to MERS-CoV spike glycoprotein [PDF]
Frontiers in Microbiology, 2022 Neutralizing monoclonal antibodies (mAbs) against highly pathogenic coronaviruses represent promising candidates for clinical intervention. Here, we isolated a potent neutralizing monoclonal antibody, MERS-S41, from a yeast displayed scFv library using ...
Shuyuan Zhang +10 more
doaj +2 more sources
Cryoelectron Microscopy Structures of AdeB Illuminate Mechanisms of Simultaneous Binding and Exporting of Substrates [PDF]
mBio, 2021 Acinetobacter ...
Christopher E. Morgan +7 more
doaj +2 more sources
The cryoelectron microscopy structure of the human CDK-activating kinase [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2020 Basil Greber +2 more
exaly +2 more sources
Macromolecular refinement of X-ray and cryoelectron microscopy structures with Phenix/OPLS3e for improved structure and ligand quality [PDF]
Structure, 2021 Nigel Moriarty +2 more
exaly +2 more sources
Capsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2019 Alexis Huet +2 more
exaly +2 more sources
Antibody-Based Affinity Cryoelectron Microscopy at 2.6-Å Resolution [PDF]
Structure, 2016 Wen Jiang, Guimei Yu, Kunpeng Li
exaly +2 more sources
High-resolution single-particle cryo-EM of samples vitrified in boiling nitrogen
IUCrJ, 2021 Based on work by Dubochet and others in the 1980s and 1990s, samples for single-particle cryo-electron microscopy (cryo-EM) have been vitrified using ethane, propane or ethane/propane mixtures.
Tyler Engstrom +7 more
doaj +1 more source