Results 1 to 10 of about 17,043 (166)

A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex

Cell Reports, 2019
Summary: LSD1 and LSD2 are homologous histone demethylases with opposite biological outcomes related to chromatin silencing and transcription elongation, respectively.
Chiara Marabelli, Biagina Marrocco, Simona Pilotto, Sagar Chittori, Sarah Picaud, Sara Marchese, Giuseppe Ciossani, Federico Forneris, Panagis Filippakopoulos, Guy Schoehn, Daniela Rhodes, Sriram Subramaniam, Andrea Mattevi   +12 more
doaj   +1 more source

Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments

Nature Communications, 2020
For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information.
Steven Z. Chou, Thomas D. Pollard
doaj   +1 more source

Tropomyosin Positions in Regulated Thin Filaments Revealed by Cryoelectron Microscopy [PDF]

Biophysical Journal, 1999
Past attempts to detect tropomyosin in electron micrograph images of frozen-hydrated troponin-regulated thin filaments under relaxing conditions have not been successful. This raised the possibility that tropomyosin may be disordered on filaments in the off-state, a possibility at odds with the steric blocking model of muscle regulation.
Xu, C., Craig, R., Tobacman, L., Horowitz, R., Lehman, W.   +4 more
openaire   +2 more sources

Protocol for the purification of replisomes from the Xenopus laevis egg extract system for single-particle cryo-EM analysis

STAR Protocols
Summary: Here, we present a large-scale FLAG immunoprecipitation protocol to isolate large protein complexes driving DNA replication at replicating chromatin assembled in Xenopus laevis egg extract.
Paolo Passaretti, Milos A. Cvetkovic, Alessandro Costa, Agnieszka Gambus   +3 more
doaj   +1 more source

Advances in Structure Modeling Methods for Cryo-Electron Microscopy Maps

Molecules, 2019
Cryo-electron microscopy (cryo-EM) has now become a widely used technique for structure determination of macromolecular complexes. For modeling molecular structures from density maps of different resolutions, many algorithms have been developed.
Eman Alnabati, Daisuke Kihara
doaj   +1 more source

Cryoelectron microscopy of low density lipoprotein in vitreous ice

Biophysical Journal, 1995
In this report, images of low density lipoprotein (LDL) in vitreous ice at approximately 30 A resolution are presented. These images show that LDL is a quasi-spherical particle, approximately 220-240 A in diameter, with a region of low density (lipid) surrounded by a ring (in projection) of high density believed to represent apolipoprotein B-100.
Spin, J.M., Atkinson, D.
openaire   +2 more sources

Cryo-EM and cryo-ET reveal the molecular architecture and host interactions of mycobacteriophage Douge

Cell Reports
Summary: Recent reports highlight the efficacy of engineered mycobacteriophages to treat non-tuberculosis mycobacterial disease. Molecular insights into mycobacteriophage architecture and host interactions could allow structure-guided phage engineering ...
Jitendra Maharana, Chun-Hsiung Wang, Li-An Tsai, Yi-Ting Liao, Cheng-Han Yang, Melvin C. Shen, Lourriel S. Macale, Thang Ngoc Tran, Joemark Narsico, Ronelito J. Perez, Sunil Kumar Tewary, Jian-Li Wu, Hong-You Lin, Shu-Wei Chang, Aaron Franklin, Patrick J. Moynihan, Deborah Jacobs-Sera, Krista G. Freeman, Graham F. Hatfull, Todd L. Lowary, Meng-Chiao Ho   +20 more
doaj   +1 more source

Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages

Bio-Protocol
A number of extracellular helical protein polymers are crucial for supporting bacterial motility. The bacterial flagellum is a polymeric appendage used to support cellular motility.
Juan Sanchez, Joseph Baumgardt, Elizabeth Wright   +2 more
doaj   +1 more source

Hypothesis for a mechanism of beam-induced motion in cryo-electron microscopy

IUCrJ, 2020
Estimates of heat-transfer rates during plunge-cooling and the patterns of ice observed in cryo-EM samples indicate that the grid bars cool much more slowly than do the support foil and sample near the middle of the grid openings. The resulting transient
Robert E. Thorne
doaj   +1 more source

Cryo-Electron Microscopy Studies of Biomolecular Structure and Dynamics

Micromachines
The technical innovation of the last decade has provided novel tools that are now transforming the field of biophysics by bringing remarkable atomic level insights into the mechanisms employed by bio-micromachines to sustain life [...]
Arkadiusz W. Kulczyk
doaj   +1 more source