Results 31 to 40 of about 17,043 (166)

Structure of the p53/RNA polymerase II assembly

Communications Biology, 2021
Liou et al. report a 4.6 Å resolution structure of the human p53/ RNA polymerase II assembly, using single particle cryoelectron microscopy. This study suggests that p53’s functional domains regulate the DNA binding activity of RNA polymerase II ...
Shu-Hao Liou, Sameer K. Singh, Robert H. Singer, Robert A. Coleman, Wei-Li Liu   +4 more
doaj   +1 more source

Cryoelectron Microscopy Reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae Type IV Pili at Sub-nanometer Resolution [PDF]

Structure, 2017
Mathieu Coureuil, Lisa Craig, Tomasz Osiński   +2 more
exaly   +2 more sources

Cryoelectron Microscopy of Vitrified Specimens [PDF]

, 1987
Water is the most abundant component of biological material, but it is systematically excluded from conventional electron microscopy. This is because water evaporates rapidly under the vacuum conditions of an electron microscope. Cryoelectron microscopy has long been seen as a possible avenue to overcome this limitation, but until recently the direct ...
Dubochet, Jacques, Adrian, Marc, Chang, Jiin-Ju, Lepault, Jean, McDowall, Alasdair W.   +4 more
openaire   +3 more sources

Structure of the CLC-1 chloride channel from Homo sapiens

eLife, 2018
CLC channels mediate passive Cl− conduction, while CLC transporters mediate active Cl− transport coupled to H+ transport in the opposite direction. The distinction between CLC-0/1/2 channels and CLC transporters seems undetectable by amino acid sequence.
Eunyong Park, Roderick MacKinnon
doaj   +1 more source

Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image simulation

Nature Communications, 2021
Understanding the biomolecular corona is of key importance to nanomedicine. Here, the authors report on cryo-electron and tomographic imaging of the corona formed on model nanoparticles and the 3D reconstruction of the corona to study the distribution ...
Sara Sheibani, Kaustuv Basu, Ali Farnudi, Aliakbar Ashkarran, Muneyoshi Ichikawa, John F. Presley, Khanh Huy Bui, Mohammad Reza Ejtehadi, Hojatollah Vali, Morteza Mahmoudi   +9 more
doaj   +1 more source

Fully three-dimensional defocus-gradient corrected backprojection in cryoelectron microscopy [PDF]

Ultramicroscopy, 2010
Joanna Klukowska, Gabor Herman, Ivan Kazantsev   +2 more
exaly   +2 more sources

Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization

Cell Reports, 2018
Summary: Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans ...
S. Saif Hasan, Chengqun Sun, Arthur S. Kim, Yasunori Watanabe, Chun-Liang Chen, Thomas Klose, Geeta Buda, Max Crispin, Michael S. Diamond, William B. Klimstra, Michael G. Rossmann   +10 more
doaj   +1 more source

Cryoelectron Microscopy of Mouse Mammary Tumor Virus [PDF]

Journal of Virology, 2004
ABSTRACT Cryoelectron microscopy of Mouse mammary tumor virus , a Betaretrovirus , provided information about glycoprotein structure and core formation. The virions showed the broad range of diameters typical of retroviruses.
Briggs, J., Watson, B., Gowen, B., Fuller, S.   +3 more
openaire   +3 more sources

Cryo-EM structure of an activated VIP1 receptor-G protein complex revealed by a NanoBiT tethering strategy

Nature Communications, 2020
Vasoactive intestinal polypeptide receptor (VIP1R) is a widely expressed class B G protein-coupled receptor and a drug target for the treatment of inflammatory diseases.
Jia Duan, Dan-dan Shen, X. Edward Zhou, Peng Bi, Qiu-feng Liu, Yang-xia Tan, You-wen Zhuang, Hui-bing Zhang, Pei-yu Xu, Si-Jie Huang, Shan-shan Ma, Xin-heng He, Karsten Melcher, Yan Zhang, H. Eric Xu, Yi Jiang   +15 more
doaj   +1 more source

Temporal dynamics of charge buildup in cryo-electron microscopy

Journal of Structural Biology: X, 2023
It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly understood.
Makoto Tokoro Schreiber, Alan Maigné, Marco Beleggia, Satoshi Shibata, Matthias Wolf   +4 more
doaj   +1 more source