Results 31 to 40 of about 1,110,843 (197)

Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization

Cell Reports, 2018
Summary: Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans ...
S. Saif Hasan, Chengqun Sun, Arthur S. Kim, Yasunori Watanabe, Chun-Liang Chen, Thomas Klose, Geeta Buda, Max Crispin, Michael S. Diamond, William B. Klimstra, Michael G. Rossmann   +10 more
doaj   +1 more source

Cryo-EM structure of an activated VIP1 receptor-G protein complex revealed by a NanoBiT tethering strategy

Nature Communications, 2020
Vasoactive intestinal polypeptide receptor (VIP1R) is a widely expressed class B G protein-coupled receptor and a drug target for the treatment of inflammatory diseases.
Jia Duan, Dan-dan Shen, X. Edward Zhou, Peng Bi, Qiu-feng Liu, Yang-xia Tan, You-wen Zhuang, Hui-bing Zhang, Pei-yu Xu, Si-Jie Huang, Shan-shan Ma, Xin-heng He, Karsten Melcher, Yan Zhang, H. Eric Xu, Yi Jiang   +15 more
doaj   +1 more source

Temporal dynamics of charge buildup in cryo-electron microscopy

Journal of Structural Biology: X, 2023
It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly understood.
Makoto Tokoro Schreiber, Alan Maigné, Marco Beleggia, Satoshi Shibata, Matthias Wolf   +4 more
doaj   +1 more source

Structure and Function of a Multi‐Megadalton Virus‐Like Proteolytic Dodecahedron

Angewandte Chemie, EarlyView.
Zuzalysin, a virulence metallopeptidase of the periodontal pathogen Porphyromonas gingivalis, is secreted as a latent, flexible ≈95‐kDa monomer. Calcium triggers cysteine‐switch activation and hierarchical assembly into pentamers, bipentamers, tripentamers, and a ≈5.6‐MDa, ≈355‐Å dodecahedron. Structures at 1.8–3.6 Å reveal a virus‐sized catalytic cage
Mariusz Madej, Arturo Rodríguez‐Banqueri, Danuta Mizgalska, Borys Szmigielski, Zuzanna Nowakowska, Małgorzata Benedyk‐Machaczka, Monika Bzowska, Katarzyna Mikruta, Juan Sebastián Ramírez‐Larrota, Chinanu Agunanne, Olivier Julien, Anthony J. O'Donoghue, Carsten Scavenius, Mario López‐Martín, Enrique Marcos, Łukasz Koziej, Sebastian Glatt, Pablo Guerra, Ulrich Eckhard, Jan Potempa, F. Xavier Gomis‐Rüth   +20 more
wiley   +2 more sources

Morphology of influenza B/Lee/40 determined by cryo-electron microscopy. [PDF]

PLoS ONE, 2014
Cryo-electron microscopy projection image analysis and tomography is used to describe the overall architecture of influenza B/Lee/40. Algebraic reconstruction techniques with utilization of volume elements (blobs) are employed to reconstruct tomograms of
Garrett Katz, Younes Benkarroum, Hui Wei, William J Rice, Doris Bucher, Alexandra Alimova, Al Katz, Joanna Klukowska, Gabor T Herman, Paul Gottlieb   +9 more
doaj   +1 more source

Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes

Cell Reports, 2021
Summary: The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism, and appetite and is an important target for treatment of type 2 diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials,
Xin Zhang, Matthew J. Belousoff, Yi-Lynn Liang, Radostin Danev, Patrick M. Sexton, Denise Wootten   +5 more
doaj   +1 more source

Correlative light electron microscopy of giant viruses with the SECOM system

New Microbes and New Infections, 2018
Bright-field transmission electron microscopy (TEM), TEM-negative staining technique, resin-embedding and ultramicrotomy, scanning TEM, scanning electron microscopy, atomic force microscopy and cryoelectron microscopy are imaging techniques used for ...
J.-P. Baudoin, S. Hari, D. Sahmi-Bounsiar, S.I. Traore, J. Bou-Khalil, J. Andréani, B. La Scola   +6 more
doaj   +1 more source

Recent advances in retroviruses via cryo-electron microscopy

Retrovirology, 2018
Cryo-electron microscopy has undergone a revolution in recent years and it has contributed significantly to a number of different areas in biological research.
Johnson Mak, Alex de Marco
doaj   +1 more source

Cryo-electron microscopy of extracellular vesicles from cerebrospinal fluid.

PLoS ONE, 2020
Extracellular vesicles (EVs) are membrane-enclosed vesicles which play important role for cell communication and physiology. EVs are found in many human biological fluids, including blood, breast milk, urine, cerebrospinal fluid (CSF), ejaculate, saliva ...
Anton Emelyanov, Tatiana Shtam, Roman Kamyshinsky, Luiza Garaeva, Nikolai Verlov, Irina Miliukhina, Anastasia Kudrevatykh, Gaspar Gavrilov, Yulia Zabrodskaya, Sofya Pchelina, Andrey Konevega   +10 more
doaj   +1 more source

Learning to automate cryo-electron microscopy data collection with Ptolemy

IUCrJ, 2023
Over the past decade, cryo-electron microscopy (cryoEM) has emerged as an important method for determining near-native, near-atomic resolution 3D structures of biological macromolecules.
Paul T. Kim, Alex J. Noble, Anchi Cheng, Tristan Bepler   +3 more
doaj   +1 more source