Results 31 to 40 of about 22,940 (199)

Cryo-EM structure of an activated VIP1 receptor-G protein complex revealed by a NanoBiT tethering strategy

Nature Communications, 2020
Vasoactive intestinal polypeptide receptor (VIP1R) is a widely expressed class B G protein-coupled receptor and a drug target for the treatment of inflammatory diseases.
Jia Duan, Dan-dan Shen, X. Edward Zhou, Peng Bi, Qiu-feng Liu, Yang-xia Tan, You-wen Zhuang, Hui-bing Zhang, Pei-yu Xu, Si-Jie Huang, Shan-shan Ma, Xin-heng He, Karsten Melcher, Yan Zhang, H. Eric Xu, Yi Jiang   +15 more
doaj   +1 more source

3-D Ultrastructure of O. tauri: Electron Cryotomography of an Entire Eukaryotic Cell [PDF]

, 2007
The hallmark of eukaryotic cells is their segregation of key biological functions into discrete, membrane-bound organelles. Creating accurate models of their ultrastructural complexity has been difficult in part because of the limited resolution of light
A Al-Amoudi, A Briegel, A Komeili, A Scheffel, AM van de Meene, AS Frangakis, AS Frangakis, B Farinas, B Palenik, B Storrie, C Courties, C Courties, C Suloway, CA Mannella, CA Mannella, CA Mannella, CA Mannella, CM Spahn, CV Iancu, CV Iancu, CW Akey, D Hoepfner, D Nicastro, D Stoffler, DN Mastronarde, E Derelle, E Shimoni, ER Wright, G Perkins, GC Johnston, GJ Jensen, GK Voeltz, GM Gaietta, GP Henderson, Grant J. Jensen, Gregory P. Henderson, HS Yoon, J Dubochet, J Kurner, JB Heymann, JL Hoog, JP Caviston, JP Ral, JR Warner, KL McDonald, LA Staehelin, LR Comolli, Lu Gan, M Beck, M Marko, MJ Chrétiennot-Dinet, O Medalia, PA Leong, Paul Digard, PE Purdue, Q Yang, QS Zheng, R Grimm, R Nevo, R Zadrag, RR Guillard, RT Mullen, S Robbens, S Subramaniam, SP Drummond, UC Vothknecht, V Lucic, V Stoppin, Y Shibata   +68 more
core   +4 more sources

A new view into prokaryotic cell biology from electron cryotomography [PDF]

, 2016
Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially native state to 'macromolecular' (~4 nm) resolution, revealing the basic architectures of complete nanomachines and their arrangements in situ.
Jensen, Grant J., Oikonomou, Catherine M.   +1 more
core   +1 more source

3D visualization of HIV virions by cryoelectron tomography. [PDF]

, 2010
The structure of the human immunodeficiency virus (HIV) and some of its components have been difficult to study in three-dimensions (3D) primarily because of their intrinsic structural variability.
Accola, Arthur, Bartesaghi, Baumeister, Beck, Benjamin, Briggs, Briggs, Briggs, Briggs, Briggs, Burton, Byeon, Chan, Chen, Chen, Dai, Douglas, Dubochet, Frangakis, Freed, Fuller, Förster, Förster, Ganser, Ganser, Ganser-Pornillos, Gay, Gelderblom, Gross, Grunewald, Harauz, Heymann, Huang, Koster, Koster, Kremer, Kwong, Lanman, Lanman, Li, Li, Liang, Liu, Liu, Liu, Lucic, Ludtke, Massiah, Massiah, Mastronarde, McEwen, Medalia, Murphy, Nermut, Nickell, Pancera, Penczek, Pettersen, Pornillos, Sougrat, Subramaniam, Subramaniam, Suloway, Walz, Wilk, Winkler, Winkler, Winkler, Wright, Ye, Zanetti, Zheng, Zhou, Zhou, Zhu, Zhu, Zhu   +77 more
core   +1 more source

Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes

Cell Reports, 2021
Summary: The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism, and appetite and is an important target for treatment of type 2 diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials,
Xin Zhang, Matthew J. Belousoff, Yi-Lynn Liang, Radostin Danev, Patrick M. Sexton, Denise Wootten   +5 more
doaj   +1 more source

Genome organization and interaction with capsid protein in a multipartite RNA virus. [PDF]

, 2020
We report the asymmetric reconstruction of the single-stranded RNA (ssRNA) content in one of the three otherwise identical virions of a multipartite RNA virus, brome mosaic virus (BMV).
Beren, Christian, Chakravarty, Antara, Cui, Yanxiang, Gelbart, William M, Knobler, Charles M, Rao, ALN, Yang, Xue, Zhou, Z Hong   +7 more
core  

Gi- and Gs-coupled GPCRs show different modes of G-protein binding. [PDF]

, 2018
More than two decades ago, the activation mechanism for the membrane-bound photoreceptor and prototypical G protein-coupled receptor (GPCR) rhodopsin was uncovered.
Altenbach, Christian, Caro, Lydia N, Dror, Ron O, Ernst, Oliver P, Hollingsworth, Scott A, Hubbell, Wayne L, Latorraca, Naomi R, Van Eps, Ned   +7 more
core   +1 more source

Correlative light electron microscopy of giant viruses with the SECOM system

New Microbes and New Infections, 2018
Bright-field transmission electron microscopy (TEM), TEM-negative staining technique, resin-embedding and ultramicrotomy, scanning TEM, scanning electron microscopy, atomic force microscopy and cryoelectron microscopy are imaging techniques used for ...
J.-P. Baudoin, S. Hari, D. Sahmi-Bounsiar, S.I. Traore, J. Bou-Khalil, J. Andréani, B. La Scola   +6 more
doaj   +1 more source

The membrane attack complex, perforin and cholesterol-dependent cytolysin superfamily of pore-forming proteins [PDF]

, 2016
The membrane attack complex and perforin proteins (MACPFs) and bacterial cholesterol-dependent cytolysins (CDCs) are two branches of a large and diverse superfamily of pore-forming proteins that function in immunity and pathogenesis.
Blackman, Bubeck, Bubeck, Cascales, Collier, Czajkowsky, Degiacomi, Dudkina, Dunstone, Estévez-Calvar, Ghatak, Giddings, Gilbert, Gilbert, Gilbert, Gilbert, Giousoh, Hadders, Hadders, Heuck, Hirst, Hotze, Hotze, Hotze, Housden, Jiang, Johnson, Johnson, Kawano, Kondos, LaChapelle, Law, Leka, Leung, Lovelace, Lukoyanova, Marchioretto, McCormack, Menestrina, Merle, Metkar, Morgan, Morgan, Mulvihill, Ounjai, Pardo-López, Peraro, Podack, Podobnik, Popoff, Praper, Ramachandran, Ramachandran, Ramm, Reboul, Reboul, Rosado, Rosado, Rossjohn, Sato, Serna, Seveau, Shatursky, Shepard, Shewell, Sonnen, Sugawara, Taylor, Tilley, Tomita, Tschopp, Tweten, Voskoboinik, Wade, Wade, Walker   +75 more
core   +2 more sources

Recent advances in retroviruses via cryo-electron microscopy

Retrovirology, 2018
Cryo-electron microscopy has undergone a revolution in recent years and it has contributed significantly to a number of different areas in biological research.
Johnson Mak, Alex de Marco
doaj   +1 more source