Developing Graphene Grids for Cryoelectron Microscopy [PDF]
Frontiers in Molecular Biosciences, 2022 Cryogenic electron microscopy (cryo-EM) single particle analysis has become one of the major techniques used to study high-resolution 3D structures of biological macromolecules.
Hongcheng Fan +5 more
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Purification and cryoelectron microscopy structure determination of human V-ATPase [PDF]
STAR Protocols, 2021 Summary: Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are multi-component, ATP-driven proton pumps, which play important roles in many physiological processes by acidifying intracellular vesicles, organelles, and the extracellular ...
Longfei Wang +3 more
doaj +2 more sources
Determining structures in a native environment using single-particle cryoelectron microscopy images [PDF]
The Innovation, 2021 Cryo-electron tomography is a powerful tool for structure determination in the native environment. However, this method requires the acquisition of tilt series, which is time-consuming and severely slows structure determination. By treating the densities
Jing Cheng +3 more
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Cryoelectron microscopy structures of a human neutralizing antibody bound to MERS-CoV spike glycoprotein [PDF]
Frontiers in Microbiology, 2022 Neutralizing monoclonal antibodies (mAbs) against highly pathogenic coronaviruses represent promising candidates for clinical intervention. Here, we isolated a potent neutralizing monoclonal antibody, MERS-S41, from a yeast displayed scFv library using ...
Shuyuan Zhang +10 more
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Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy. [PDF]
J Vis Exp, 2023 Cryogenic electron microscopy (cryoEM) has emerged as a powerful technique for probing the atomic structure of macromolecular complexes. Sample preparation for cryoEM requires preserving specimens in a thin layer of vitreous ice, typically suspended within the holes of a fenestrated support film. However, all commonly used sample preparation approaches
Basanta B, Chen W, Pride DE, Lander GC.
europepmc +3 more sources
Structure of the AAVhu.37 capsid by cryoelectron microscopy. [PDF]
Acta Crystallogr F Struct Biol Commun, 2020 Adeno-associated viruses (AAVs) are used as in vivo gene-delivery vectors in gene-therapy products and have been heavily investigated for numerous indications. Over 100 naturally occurring AAV serotypes and variants have been isolated from primate samples.
Kaelber JT +6 more
europepmc +4 more sources
What is in the black box? - A perspective on software in cryoelectron microscopy. [PDF]
Biophys J, 2021 This article bemoans the demise of truly modular open-source image processing systems, such as SPIDER, in recent years' development of tools for three-dimensional reconstruction in cryo-electron microscopy. Instead, today's users have to rely on the functionality of software systems that have little or no transparency.
Maji S, Frank J.
europepmc +3 more sources
Cryoelectron Microscopy Structures of AdeB Illuminate Mechanisms of Simultaneous Binding and Exporting of Substrates [PDF]
mBio, 2021 Acinetobacter ...
Christopher E. Morgan +7 more
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Residue-level error detection in cryoelectron microscopy models. [PDF]
Structure, 2023 Building accurate protein models into moderate resolution (3-5 Å) cryoelectron microscopy (cryo-EM) maps is challenging and error prone. We have developed MEDIC (Model Error Detection in Cryo-EM), a robust statistical model that identifies local backbone errors in protein structures built into cryo-EM maps by combining local fit-to-density with deep ...
Reggiano G +4 more
europepmc +4 more sources
iMEM: Isolation of Plasma Membrane for Cryoelectron Microscopy [PDF]
Structure, 2016 The plasma membrane and the cell cortex are essential parts of the eukaryotic cell. The plasma membrane delimitates the cell and mediates communication with the outside. The cell cortex is the submembrane cytoskeleton shaping the cell and is able to reorganize for the passage of material.
Benoit Zuber
exaly +4 more sources