Results 11 to 20 of about 22,940 (199)
High-pressure freezing for cryoelectron microscopy [PDF]
Trends in Cell Biology, 1995 Dubochet, J.
core +4 more sources
Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy. [PDF]
J Vis Exp, 2023 Cryogenic electron microscopy (cryoEM) has emerged as a powerful technique for probing the atomic structure of macromolecular complexes. Sample preparation for cryoEM requires preserving specimens in a thin layer of vitreous ice, typically suspended within the holes of a fenestrated support film. However, all commonly used sample preparation approaches
Basanta B, Chen W, Pride DE, Lander GC.
europepmc +3 more sources
Structure of the AAVhu.37 capsid by cryoelectron microscopy. [PDF]
Acta Crystallogr F Struct Biol Commun, 2020 Adeno-associated viruses (AAVs) are used as in vivo gene-delivery vectors in gene-therapy products and have been heavily investigated for numerous indications. Over 100 naturally occurring AAV serotypes and variants have been isolated from primate samples.
Kaelber JT +6 more
europepmc +4 more sources
What is in the black box? - A perspective on software in cryoelectron microscopy. [PDF]
Biophys J, 2021 This article bemoans the demise of truly modular open-source image processing systems, such as SPIDER, in recent years' development of tools for three-dimensional reconstruction in cryo-electron microscopy. Instead, today's users have to rely on the functionality of software systems that have little or no transparency.
Maji S, Frank J.
europepmc +3 more sources
Residue-level error detection in cryoelectron microscopy models. [PDF]
Structure, 2023 Building accurate protein models into moderate resolution (3-5 Å) cryoelectron microscopy (cryo-EM) maps is challenging and error prone. We have developed MEDIC (Model Error Detection in Cryo-EM), a robust statistical model that identifies local backbone errors in protein structures built into cryo-EM maps by combining local fit-to-density with deep ...
Reggiano G +4 more
europepmc +4 more sources
The cryoelectron microscopy structure of the human CDK-activating kinase. [PDF]
Proc Natl Acad Sci U S A, 2020 Significance Control of gene expression and the cell cycle is critical for appropriate cell growth and timely cell division. Failure of the mechanisms regulating these processes can result in proliferative diseases. A molecular complex termed the CDK-activating kinase (CAK) impinges on both of these regulatory networks in human cells ...
Greber BJ +5 more
europepmc +6 more sources
Nature Communications, 2021 Topochemical polymerization reactions produce ultra-high molecular weight crystalline polymers. Here the authors show a family of para-azaquinodimethane compounds that undergo facile visible light and thermally initiated polymerization in the solid state
Christopher L. Anderson +13 more
doaj +1 more source
CryoSeek: A strategy for bioentity discovery using cryoelectron microscopy. [PDF]
Proc Natl Acad Sci U S AStructural biology is experiencing a paradigm shift from targeted structural determination to structure-guided discovery of previously uncharacterized bioentities. We employed cryoelectron microscopy (cryo-EM) to analyze filtered water samples collected from the Tsinghua Lotus Pond.
Wang T +6 more
europepmc +3 more sources
Oxygen-Sensitive Metalloprotein Structure Determination by Cryo-Electron Microscopy
Biomolecules, 2022 Metalloproteins are involved in key cell processes such as photosynthesis, respiration, and oxygen transport. However, the presence of transition metals (notably iron as a component of [Fe-S] clusters) often makes these proteins sensitive to oxygen ...
Mickaël V. Cherrier +8 more
doaj +1 more source
Structure of the p53/RNA polymerase II assembly
Communications Biology, 2021 Liou et al. report a 4.6 Å resolution structure of the human p53/ RNA polymerase II assembly, using single particle cryoelectron microscopy. This study suggests that p53’s functional domains regulate the DNA binding activity of RNA polymerase II ...
Shu-Hao Liou +4 more
doaj +1 more source