Results 21 to 30 of about 22,654 (207)

Structure of the CLC-1 chloride channel from Homo sapiens

eLife, 2018
CLC channels mediate passive Cl− conduction, while CLC transporters mediate active Cl− transport coupled to H+ transport in the opposite direction. The distinction between CLC-0/1/2 channels and CLC transporters seems undetectable by amino acid sequence.
Eunyong Park, Roderick MacKinnon
doaj   +1 more source

Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image simulation

Nature Communications, 2021
Understanding the biomolecular corona is of key importance to nanomedicine. Here, the authors report on cryo-electron and tomographic imaging of the corona formed on model nanoparticles and the 3D reconstruction of the corona to study the distribution ...
Sara Sheibani, Kaustuv Basu, Ali Farnudi, Aliakbar Ashkarran, Muneyoshi Ichikawa, John F. Presley, Khanh Huy Bui, Mohammad Reza Ejtehadi, Hojatollah Vali, Morteza Mahmoudi   +9 more
doaj   +1 more source

Bionanomaterials from plant viruses [PDF]

, 2015
Plant virus capsids have emerged as useful biotemplates for material synthesis. All plant virus capsids are assembled with high-precision, three-dimensional structures providing nanoscale architectures that are highly monodisperse, can be produced in ...
Aljabali, Alaa A. A., Evans, David
core   +2 more sources

Temporal dynamics of charge buildup in cryo-electron microscopy

Journal of Structural Biology: X, 2023
It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly understood.
Makoto Tokoro Schreiber, Alan Maigné, Marco Beleggia, Satoshi Shibata, Matthias Wolf   +4 more
doaj   +1 more source

Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization

Cell Reports, 2018
Summary: Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans ...
S. Saif Hasan, Chengqun Sun, Arthur S. Kim, Yasunori Watanabe, Chun-Liang Chen, Thomas Klose, Geeta Buda, Max Crispin, Michael S. Diamond, William B. Klimstra, Michael G. Rossmann   +10 more
doaj   +1 more source

Cryo-EM structure of an activated VIP1 receptor-G protein complex revealed by a NanoBiT tethering strategy

Nature Communications, 2020
Vasoactive intestinal polypeptide receptor (VIP1R) is a widely expressed class B G protein-coupled receptor and a drug target for the treatment of inflammatory diseases.
Jia Duan, Dan-dan Shen, X. Edward Zhou, Peng Bi, Qiu-feng Liu, Yang-xia Tan, You-wen Zhuang, Hui-bing Zhang, Pei-yu Xu, Si-Jie Huang, Shan-shan Ma, Xin-heng He, Karsten Melcher, Yan Zhang, H. Eric Xu, Yi Jiang   +15 more
doaj   +1 more source

3-D Ultrastructure of O. tauri: Electron Cryotomography of an Entire Eukaryotic Cell [PDF]

, 2007
The hallmark of eukaryotic cells is their segregation of key biological functions into discrete, membrane-bound organelles. Creating accurate models of their ultrastructural complexity has been difficult in part because of the limited resolution of light
A Al-Amoudi, A Briegel, A Komeili, A Scheffel, AM van de Meene, AS Frangakis, AS Frangakis, B Farinas, B Palenik, B Storrie, C Courties, C Courties, C Suloway, CA Mannella, CA Mannella, CA Mannella, CA Mannella, CM Spahn, CV Iancu, CV Iancu, CW Akey, D Hoepfner, D Nicastro, D Stoffler, DN Mastronarde, E Derelle, E Shimoni, ER Wright, G Perkins, GC Johnston, GJ Jensen, GK Voeltz, GM Gaietta, GP Henderson, Grant J. Jensen, Gregory P. Henderson, HS Yoon, J Dubochet, J Kurner, JB Heymann, JL Hoog, JP Caviston, JP Ral, JR Warner, KL McDonald, LA Staehelin, LR Comolli, Lu Gan, M Beck, M Marko, MJ Chrétiennot-Dinet, O Medalia, PA Leong, Paul Digard, PE Purdue, Q Yang, QS Zheng, R Grimm, R Nevo, R Zadrag, RR Guillard, RT Mullen, S Robbens, S Subramaniam, SP Drummond, UC Vothknecht, V Lucic, V Stoppin, Y Shibata   +68 more
core   +4 more sources

iMEM: Isolation of Plasma Membrane for Cryoelectron Microscopy [PDF]

Structure, 2016
The plasma membrane and the cell cortex are essential parts of the eukaryotic cell. The plasma membrane delimitates the cell and mediates communication with the outside. The cell cortex is the submembrane cytoskeleton shaping the cell and is able to reorganize for the passage of material.
Camille Françoise, Peitsch, Sven, Beckmann, Benoît, Zuber   +2 more
openaire   +2 more sources

Gi- and Gs-coupled GPCRs show different modes of G-protein binding. [PDF]

, 2018
More than two decades ago, the activation mechanism for the membrane-bound photoreceptor and prototypical G protein-coupled receptor (GPCR) rhodopsin was uncovered.
Altenbach, Christian, Caro, Lydia N, Dror, Ron O, Ernst, Oliver P, Hollingsworth, Scott A, Hubbell, Wayne L, Latorraca, Naomi R, Van Eps, Ned   +7 more
core   +1 more source

A new view into prokaryotic cell biology from electron cryotomography [PDF]

, 2016
Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially native state to 'macromolecular' (~4 nm) resolution, revealing the basic architectures of complete nanomachines and their arrangements in situ.
Jensen, Grant J., Oikonomou, Catherine M.   +1 more
core   +1 more source