Results 21 to 30 of about 17,043 (166)
The cryoelectron microscopy structure of the human CDK-activating kinase. [PDF]
Proc Natl Acad Sci U S A, 2020 Significance Control of gene expression and the cell cycle is critical for appropriate cell growth and timely cell division. Failure of the mechanisms regulating these processes can result in proliferative diseases. A molecular complex termed the CDK-activating kinase (CAK) impinges on both of these regulatory networks in human cells ...
Greber BJ +5 more
europepmc +6 more sources
CryoSeek: A strategy for bioentity discovery using cryoelectron microscopy. [PDF]
Proc Natl Acad Sci U S AStructural biology is experiencing a paradigm shift from targeted structural determination to structure-guided discovery of previously uncharacterized bioentities. We employed cryoelectron microscopy (cryo-EM) to analyze filtered water samples collected from the Tsinghua Lotus Pond.
Wang T +6 more
europepmc +3 more sources
Quaternary organization of a phytochrome dimer as revealed by cryoelectron microscopy. [PDF]
Proc Natl Acad Sci U S A, 2010 Phytochromes are a collection of dimeric photoreceptors that direct a diverse array of responses in plants and microorganisms through photoconversion between a red light-absorbing ground state Pr, and a far-red light-absorbing photoactivated state Pfr.
Li H, Zhang J, Vierstra RD, Li H.
europepmc +4 more sources
Macromolecular refinement of X-ray and cryoelectron microscopy structures with Phenix/OPLS3e for improved structure and ligand quality [PDF]
Structure, 2021 Nigel Moriarty +2 more
exaly +2 more sources
High-resolution single-particle cryo-EM of samples vitrified in boiling nitrogen
IUCrJ, 2021 Based on work by Dubochet and others in the 1980s and 1990s, samples for single-particle cryo-electron microscopy (cryo-EM) have been vitrified using ethane, propane or ethane/propane mixtures.
Tyler Engstrom +7 more
doaj +1 more source
Antibody-Based Affinity Cryoelectron Microscopy at 2.6-Å Resolution [PDF]
Structure, 2016 Wen Jiang, Guimei Yu, Kunpeng Li
exaly +2 more sources
Artificial Intelligence in Cryo-Electron Microscopy
Life, 2022 Cryo-electron microscopy (cryo-EM) has become an unrivaled tool for determining the structure of macromolecular complexes. The biological function of macromolecular complexes is inextricably tied to the flexibility of these complexes.
Jeong Min Chung +2 more
doaj +1 more source
Mechanistic understanding of human SLFN11
Nature Communications, 2022 Schlafen 11 serves as an antiviral restriction factor and a predictive biomarker in cancer. Here, the authors use cryoelectron microscopy and biochemical assays to understand tRNA endoribonuclease and DNA binding functions of human Schlafen 11.
Felix J. Metzner +5 more
doaj +1 more source
Nature Communications, 2021 Topochemical polymerization reactions produce ultra-high molecular weight crystalline polymers. Here the authors show a family of para-azaquinodimethane compounds that undergo facile visible light and thermally initiated polymerization in the solid state
Christopher L. Anderson +13 more
doaj +1 more source
Oxygen-Sensitive Metalloprotein Structure Determination by Cryo-Electron Microscopy
Biomolecules, 2022 Metalloproteins are involved in key cell processes such as photosynthesis, respiration, and oxygen transport. However, the presence of transition metals (notably iron as a component of [Fe-S] clusters) often makes these proteins sensitive to oxygen ...
Mickaël V. Cherrier +8 more
doaj +1 more source