A discussion of cryo-EM terminology as the outreach and number of PDB entries expand [PDF]
IUCrJCryo electron microscopy (cryo-EM) has made great advances in the last decade, progressively increasing its impact in structural biology as a key method to address molecular structures and mechanisms of various macromolecular complexes.
Bruno P. Klaholz
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Cryo-EM structure of a protein nanopore reveals lipid acyl chains as integral components [PDF]
Sci AdvAshworth M +4 more
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Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching. [PDF]
ElifeZhang K, Grant T, Grigorieff N.
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In extracto cryo-EM reveals eEF2 as a major hibernation factor on 60S and 80S particles [PDF]
eLifeCryogenic electron microscopy (cryo-EM) made impressive progress in resolving cellular macromolecules and their detailed interactions. Single-particle cryo-EM traditionally relies on purified macromolecules and lacks the complexity of cellular ...
Zahra Seraj +7 more
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Best practice: setting up and operating a mid-sized cryo-EM facility
Frontiers in Molecular Biosciences, 2023 Ever since the resolution revolution in 2013, cryo-electron microscopy (cryo-EM) has become a powerful methodology in structural biology that is especially suited to study the structure of large flexible molecular complexes.
Xing Meng +8 more
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TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages
Scientific Reports, 2021 Identifying endogenous tissue stem cells remains a key challenge in developmental and regenerative biology. To distinguish and molecularly characterise stem cell populations in large heterogeneous tissues, the combination of cytochemical cell markers ...
Viola Oorschot +3 more
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Practices for running a research-oriented shared cryo-EM facility
Frontiers in Molecular Biosciences, 2022 The Harvard Cryo-Electron Microscopy Center for Structural Biology, which was formed as a consortium between Harvard Medical School, Boston Children’s Hospital, Dana-Farber Cancer Institute, and Massachusetts General Hospital, serves both academic and ...
Richard M. Walsh +14 more
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The Ewald sphere/focus gradient does not limit the resolution of cryoEM reconstructions
Journal of Structural Biology: X, 2023 In our quest to solve biomolecular structures to higher resolutions in cryoEM, care must be taken to deal with all aspects of image formation in the electron microscope.
J. Bernard Heymann
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BRCA1-A and BRISC: Multifunctional Molecular Machines for Ubiquitin Signaling
Biomolecules, 2020 The K63-linkage specific deubiquitinase BRCC36 forms the core of two multi-subunit deubiquitination complexes: BRCA1-A and BRISC. BRCA1-A is recruited to DNA repair foci, edits ubiquitin signals on chromatin, and sequesters BRCA1 away from the site of ...
Julius Rabl
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Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states
Nature Communications, 2023 Herpesviruses remain a burden for animal and human health, including the medically important varicella-zoster virus (VZV). Membrane fusion mediated by conserved core glycoproteins, the fusogen gB and the heterodimer gH-gL, enables herpesvirus cell entry.
Momei Zhou +7 more
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