Results 31 to 40 of about 99,841 (293)
Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion. [PDF]
, 2012 The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle-vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Brunger, Axel T +13 more
core +1 more source
Routine sub-2.5 Å cryo-EM structure determination of GPCRs
Nature Communications, 2021 There is a need to optimise cryo-EM data acquisition approaches to improve the resolution of GPCR cryo-EM structures to better than 2.5 Å, in order to use them for structure-based drug design purposes.
Radostin Danev +6 more
doaj +1 more source
Structural visualization of key steps in human transcription initiation. [PDF]
, 2013 Eukaryotic transcription initiation requires the assembly of general transcription factors into a pre-initiation complex that ensures the accurate loading of RNA polymerase II (Pol II) at the transcription start site. The molecular mechanism and function
Fang, Jie +3 more
core +1 more source
Frontiers in Molecular Biosciences, 2021 Single-particle cryogenic electron microscopy (cryo-EM) has revolutionized the field of the structural biology, providing an access to the atomic resolution structures of large biomolecular complexes in their near-native environment. Today’s cryo-EM maps
Łukasz Nierzwicki +2 more
doaj +1 more source
Unsupervised cryo-EM data clustering through adaptively constrained K-means algorithm
, 2016 In single-particle cryo-electron microscopy (cryo-EM), K-means clustering algorithm is widely used in unsupervised 2D classification of projection images of biological macromolecules.
Mao, Youdong +3 more
core +7 more sources
Microscale Fluid Behavior during Cryo-EM Sample Blotting [PDF]
, 2020 Blotting has been the standard technique for preparing aqueous samples for single-particle electron cryo-microscopy for over three decades. This technique removes the excess solution from a transmission electron microscope grid by pressing absorbent ...
Armstrong, Maxim +5 more
core +1 more source
Structure of the full-length TRPV2 channel by cryo-EM. [PDF]
, 2016 Transient receptor potential (TRP) proteins form a superfamily Ca(2+)-permeable cation channels regulated by a range of chemical and physical stimuli. Structural analysis of a 'minimal' TRP vanilloid subtype 1 (TRPV1) elucidated a mechanism of channel ...
Cohen, Matthew R +6 more
core +2 more sources
Medicines, 2019 Background: Cryo-electron microscopy (cryo-EM) has become a major technique for protein structure determination. However, due to the low quality of cryo-EM density maps, many protein structures derived from cryo-EM contain outliers introduced during the ...
Lin Chen +4 more
doaj +1 more source
Evolving data standards for cryo-EM structures
Structural Dynamics, 2020 Electron cryo-microscopy (cryo-EM) is increasingly being used to determine 3D structures of a broad spectrum of biological specimens from molecules to cells. Anticipating this progress in the early 2000s, an international collaboration of scientists with
Catherine L. Lawson +2 more
doaj +1 more source
A saposin-lipoprotein nanoparticle system for membrane proteins. [PDF]
, 2016 A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with ...
Armache, Jean-Paul +13 more
core +2 more sources