Results 31 to 40 of about 6,328,527 (298)
Subtomogram analysis using the Volta phase plate [PDF]
, 2016 Cryo-electron tomography (CET) and subtomogram analysis allow studying the structures of macromolecular complexes in their natural context. The radiation sensitivity of vitrified biological specimens and the resulting low signal-to-noise ratio (SNR) in ...
Baumeister, Wolfgang +11 more
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Cryo-EM in drug discovery [PDF]
Biochemical Society Transactions, 2019 Abstract The impact of structural biology on drug discovery is well documented, and the workhorse technique for the past 30 years or so has been X-ray crystallography. With the advent of several technological improvements, including direct electron detectors, automation, better microscope vacuums and lenses, phase plates and ...
Tom Ceska +4 more
openaire +2 more sources
Proteins: Structure, Function, and Bioinformatics, 2021 Abstract Structures of seven CASP14 targets were determined using cryo‐electron microscopy (cryo‐EM) technique with resolution between 2.1 and 3.8 Å. We provide an evaluation of the submitted models versus the experimental data (cryo‐EM density maps) and experimental reference structures built into the maps.
Tristan Cragnolini +2 more
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IUCrJ, 2020 The application of cryo-EM methods to study SARS-CoV-2 proteins provides a convincing demonstration of the power of cryo-EM in the arsenal of structural biology.
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Cryo-EM structural determination of PfFNT.
, 2021 (A) A representative cryo-EM micrograph of PfFNT. (B) A representative 2D classification average. (C) Flowchart for EM data processing of PfFNT datasets. Details can be found in the Methods. cryo-EM, cryogenic-electron microscopy; EM, electron microscopy;
Yanxia Zhou (1447465) +17 more
core +1 more source
Cryo‐EM structures of coagulation factors
Research and Practice in Thrombosis and Haemostasis, 2022 A State of the Art lecture titled “Cryo‐EM structures of coagulation factors” was presented at the ISTH Congress in 2022. Cryogenic electron microscopy (cryo‐EM) is a revolutionary technique capable of solving the structure of high molecular weight ...
Enrico Di Cera +3 more
doaj +1 more source
Frontiers in Molecular Biosciences, 2022 An increasing number of protein complex structures are determined by cryo-electron microscopy (cryo-EM). When individual protein structures have been determined and are available, an important task in structure modeling is to fit the individual ...
Eman Alnabati +4 more
doaj +1 more source
Cryo-EM data, refinement, and validations.
, 2022 Cryo-EM data, refinement, and validations.
Gerald S. Baron (300505) +10 more
core +1 more source
Interpreting the cryo-EM map [PDF]
IUCrJ, 2019 Beckers et al. [IUCrJ (2019), 6, 18-33] propose a general approach to visualization of cryo-EM maps. Their method overcomes some of the challenges inherent in the conventional approach for depicting maps using a single isosurface threshold.
openaire +3 more sources
Scientific Video Protocols, 2020 Richard Henderson discusses Jacques Dubochet's, Joachim Frank's and his contributions to the development of electron cryo-microscopy.
Richard Henderson, Mejd Alsari
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