Results 41 to 50 of about 6,313,615 (343)

A Bayesian approach to extracting free-energy profiles from cryo-electron microscopy experiments

Scientific Reports, 2021
Cryo-electron microscopy (cryo-EM) extracts single-particle density projections of individual biomolecules. Although cryo-EM is widely used for 3D reconstruction, due to its single-particle nature it has the potential to provide information about a ...
Julian Giraldo-Barreto, Sebastian Ortiz, Erik H. Thiede, Karen Palacio-Rodriguez, Bob Carpenter, Alex H. Barnett, Pilar Cossio   +6 more
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Getting Started in Cryo-EM

, 2015
These video lectures by Professor Grant Jensen cover the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image ...
Jensen, Grant J.
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A Visualization Tool for Cryo-EM Protein Validation with an Unsupervised Machine Learning Model in Chimera Platform

Medicines, 2019
Background: Cryo-electron microscopy (cryo-EM) has become a major technique for protein structure determination. However, due to the low quality of cryo-EM density maps, many protein structures derived from cryo-EM contain outliers introduced during the ...
Lin Chen, Brandon Baker, Eduardo Santos, Michell Sheep, Darius Daftarian   +4 more
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Biological Breakthroughs and Drug Discovery Revolution via Cryo-Electron Microscopy of Membrane Proteins

Membranes
The application of cryo-electron microscopy (cryo-EM) in membrane protein structural biology has catalyzed unprecedented advances in our understanding of fundamental biological processes and transformed drug discovery paradigms.
Vitor Hugo Balasco Serrão
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Routine sub-2.5 Å cryo-EM structure determination of GPCRs

Nature Communications, 2021
There is a need to optimise cryo-EM data acquisition approaches to improve the resolution of GPCR cryo-EM structures to better than 2.5 Å, in order to use them for structure-based drug design purposes.
Radostin Danev, Matthew Belousoff, Yi-Lynn Liang, Xin Zhang, Fabian Eisenstein, Denise Wootten, Patrick M. Sexton   +6 more
doaj   +1 more source

Integrative approaches in cryogenic electron microscopy: Recent advances in structural biology and future perspectives

iScience, 2021
Summary: Cellular factories engage numerous highly complex “molecular machines” to perform pivotal biological functions. 3D structural visualization is an effective way to understand the functional mechanisms of these biomacromolecules.
Aneek Banerjee, Sayan Bhakta, Jayati Sengupta   +2 more
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Cryo-EM processing of the mACE2/BA.2.12.1 complex.

, 2023
Cryo-EM processing of the mACE2/BA.2.12.1 complex.
Bertrand Beckert (7941551), Alexander Myasnikov (15188359), Sergey Nazarov (3227898), Kelvin Lau (1847764), Dongchun Ni (15188356), Didier Trono (25360), Emiko Uchikawa (479329), Florence Pojer (133479), Henning Stahlberg (1802443), Priscilla Turelli (272671)   +9 more
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Cryo‐electron microscopy of cholinesterases, present and future [PDF]

, 2021
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) exist in a variety of oligomeric forms, each with defined cellular and subcellular distributions.
Leung, Miguel Ricardo, Tzviya Zeev‐Ben‐Mordehai, Miguel Ricardo Leung, Zeev-Ben-Mordehai, Tzviya, Sub Structural Biochemistry, Cryo-EM, Structural Biochemistry, Sub Cryo - EM   +7 more
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Cryogenic electron tomography reveals herpesvirus capsid assembly intermediates inside the cell nucleus

Nature Communications
Herpesviruses encapsulate their double-stranded DNA (dsDNA) genomes within an icosahedral nucleocapsid formed in the infected cell nucleus. Four biochemically purified nucleocapsids have been characterized, but their roles in herpesvirus replication ...
Stefan L. Oliver, Muyuan Chen, Leeya Engel, Corey W. Hecksel, Xueting Zhou, Michael F. Schmid, Ann M. Arvin, Wah Chiu   +7 more
doaj   +1 more source

Science in the News The Rise of Cryo-EM [PDF]

, 2018
Anderson, M. The History of Cryo-EM. https://www.fei.com/life-sciences/history-of-cryo-em/ (accessed Sep 15, 2018)
Vermillion, Austin J
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