Results 51 to 60 of about 6,328,527 (298)

Molecular Dynamics to Predict Cryo-EM: Capturing Transitions and Short-Lived Conformational States of Biomolecules

Frontiers in Molecular Biosciences, 2021
Single-particle cryogenic electron microscopy (cryo-EM) has revolutionized the field of the structural biology, providing an access to the atomic resolution structures of large biomolecular complexes in their near-native environment. Today’s cryo-EM maps
Łukasz Nierzwicki, Giulia Palermo, Giulia Palermo   +2 more
doaj   +1 more source

In Situ Studies of Mitochondrial Translation by Cryo-Electron Tomography

, 2021
Cryo-electron tomography (cryo-ET) enables the three-dimensional (3D) visualization of macromolecular complexes in their native environment (in situ).
Englmeier, Robert, Cryo-EM, Förster, Friedrich, Sub Cryo - EM   +3 more
core  

High-throughput high-resolution cryo-electron crystallography [PDF]

, 2015
High-resolution structures of membrane and soluble proteins can be obtained by cryo-electron crystallography, given highly-ordered cryo-preparations of perfectly flat 2D crystals are available.
Scherer, Sebastian
core   +1 more source

Cryo-EM analysis of MmpL3-GDN.

, 2021
(a) Data processing flow chart with particle distributions. The black box indicates the particle class used for further refinement. (b) Representative 2D classes. (c) FSC curves.
Christopher E. Morgan (11269227), Philip A. Klenotic (11269221), Meinan Lyu (11269224), Edward W. Yu (9745721), Chih-Chia Su (573213), Meng Cui (29943)   +5 more
core   +1 more source

Cryogenic electron tomography reveals herpesvirus capsid assembly intermediates inside the cell nucleus

Nature Communications
Herpesviruses encapsulate their double-stranded DNA (dsDNA) genomes within an icosahedral nucleocapsid formed in the infected cell nucleus. Four biochemically purified nucleocapsids have been characterized, but their roles in herpesvirus replication ...
Stefan L. Oliver, Muyuan Chen, Leeya Engel, Corey W. Hecksel, Xueting Zhou, Michael F. Schmid, Ann M. Arvin, Wah Chiu   +7 more
doaj   +1 more source

Evolving data standards for cryo-EM structures

Structural Dynamics, 2020
Electron cryo-microscopy (cryo-EM) is increasingly being used to determine 3D structures of a broad spectrum of biological specimens from molecules to cells. Anticipating this progress in the early 2000s, an international collaboration of scientists with
Catherine L. Lawson, Helen M. Berman, Wah Chiu   +2 more
doaj   +1 more source

Science in the News The Rise of Cryo-EM [PDF]

, 2018
Anderson, M. The History of Cryo-EM. https://www.fei.com/life-sciences/history-of-cryo-em/ (accessed Sep 15, 2018)
Vermillion, Austin J
core  

Cryo-EM data collection and refinement statistics of PfFNT.

, 2021
cryo-EM, cryo-electron microscopy; PfFNT, P. falciparum formate–nitrite transporter. (DOCX)
Yanxia Zhou (1447465), Jialu Li (506281), Huaichuan Duan (6458477), Nan Wang (21935), Chen Wang (88408), Dong Deng (8202849), Qingjie Xiao (9226140), Li Guo (171454), Weidan Yuan (10711212), Xin Jiang (6348), Zhi-jun Jia (11413904), Jianping Hu (287208), Chuangye Yan (554009), Xi Peng (406437), Angqi Zhu (8681169), Jia Geng (1708321), Hanwen Xu (6332030), Fei Liu (21082)   +17 more
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Using cryo-electron microscopy maps for X-ray structure determination

IUCrJ, 2018
X-ray crystallography and cryo-electron microscopy (cryo-EM) are complementary techniques for structure determination. Crystallography usually reveals more detailed information, while cryo-EM is an extremely useful technique for studying large-sized ...
Lingxiao Zeng, Wei Ding, Quan Hao
doaj   +1 more source