Results 51 to 60 of about 99,841 (293)

Structures of the Escherichia coli type 1 pilus during pilus rod assembly and after assembly termination

Nature Communications
Uropathogenic Escherichia coli strains use filamentous type 1 pili to adhere to and invade uroepithelial cells. The pilus consists of a flexible tip fibrillum, formed by the adhesin FimH and the subunits FimG and FimF. The pilus rod is a helical assembly
Paul Bachmann, Pavel Afanasyev, Daniel Boehringer, Rudi Glockshuber   +3 more
doaj   +1 more source

Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud

eLife, 2015
The advent of a new generation of electron microscopes and direct electron detectors has realized the potential of single particle cryo-electron microscopy (cryo-EM) as a technique to generate high-resolution structures.
Michael A Cianfrocco, Andres E Leschziner   +1 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Structure of a bacterial type IV secretion core complex at subnanometre resolution [PDF]

, 2013
Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4.
Chandran, Vidya, Fronzes, Remi, Laeremans, T., Lian, Pei W., Orlova, Elena, Pardon, E., Remaut, Han, Rivera-Calzada, A., Savva, Christos G., Steyaert, J., Waksman, Gabriel   +10 more
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

C-SPAM: an open-source time-resolved specimen vitrification device with light-activated molecules

IUCrJ
Molecular structures can be determined in vitro and in situ with cryo-electron microscopy (cryo-EM). Specimen preparation is a major obstacle in cryo-EM. Typical sample preparation is orders of magnitude slower than biological processes.
Alejandra Montaño Romero, Calli Bonin, Edward C. Twomey   +2 more
doaj   +1 more source

Diverse Morphology and Structural Features of Old and New World Hantaviruses

Viruses, 2019
To further understanding of the structure and morphology of the Orthohantavirus, family Hantaviridae, we have employed cryo-electron microscopy (cryo-EM) for three New World hantaviruses: Andes (ANDV), Sin Nombre (SNV), and Black Creek Canal (BCCV ...
Amar Parvate, Evan P. Williams, Mariah K. Taylor, Yong-Kyu Chu, Jason Lanman, Erica Ollmann Saphire, Colleen B. Jonsson   +6 more
doaj   +1 more source

Orthogonal Matrix Retrieval in Cryo-Electron Microscopy

, 2015
In single particle reconstruction (SPR) from cryo-electron microscopy (cryo-EM), the 3D structure of a molecule needs to be determined from its 2D projection images taken at unknown viewing directions.
Bhamre, Tejal, Singer, Amit, Zhang, Teng
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Cryo-EM of nucleosome core particle interactions in trans

Scientific Reports, 2018
Nucleosomes, the basic unit of chromatin, are repetitively spaced along DNA and regulate genome expression and maintenance. The long linear chromatin molecule is extensively condensed to fit DNA inside the nucleus.
Silvija Bilokapic, Mike Strauss, Mario Halic   +2 more
doaj   +1 more source