Results 71 to 80 of about 6,313,615 (343)
FEBS Letters, EarlyView.Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley +1 more source
Nature CommunicationsMycobacteria harbor a proteasome that was acquired by Actinobacteria through horizontal gene transfer and that supports the persistence of the human pathogen Mycobacterium tuberculosis within host macrophages. The core particle of the proteasome (20S CP)
Tatjana von Rosen +7 more
doaj +1 more source
The Triplex-Centric Assembly and Maturation of the Herpesvirus Procapsid
VirusesHerpesviruses are prevalent infectious agents in humans, with complex structures and life cycles. The viability and detail of a model of capsid assembly and maturation can now be examined against the recently available mature herpesvirus capsids ...
J. Bernard Heymann
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Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy
FEBS Letters, EarlyView.The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley +1 more source
IUCrJCryoEM reconstructions must be performed along the Ewald spheres to achieve resolutions beyond the projection approximation limit. The linear theory of image formation models the scattering from the specimen and focusing by the objective lens as two ...
J. Bernard Heymann
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Expanding capabilities and infrastructure for cryo-EM [PDF]
Nature Methods, 2021 Many challenges and considerations must be evaluated when expanding and supporting new cryo-electron microscopy facilities.
openaire +2 more sources
Cryo-EM data collection, model refinement, and validation statistics.
, 2023 Cryo-EM data collection, model refinement, and validation statistics.
Guibing Liu (16986486) +4 more
core +1 more source
FEBS Letters, EarlyView.Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana +3 more
wiley +1 more source
A cryo-electron microscopic approach to elucidate protein structures from human brain microsomes
Life Science Alliance, 2023 Using cryo-EM to simultaneously identify and solve structures of five enzymes from a raw human brain microsomal lysate, highlighting the potential for facilitating structural-omics at the atomic level.
Marios L Tringides +4 more
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Comparing the paths of Hedgehog release in artificial and natural settings
FEBS Letters, EarlyView.Hh morphogens require solubilization factors to overcome their tight association with the plasma membrane and signal to distant cells, but the precise functions of these factors are controversial. Here, we use published in vivo observations as the most relevant evidence to test their suggested functions and discuss contradictions and gaps between in ...
Janna Puschmann, Kay Grobe
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