Results 71 to 80 of about 6,328,527 (298)
Nature Methods, 2015 Gold supports nearly eliminate unwanted specimen motion in cryo-electron microscopy, enabling high-resolution structure determination of challenging proteins.
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Cryo-EM structure of the exocyst complex [PDF]
Nature Structural & Molecular Biology, 2018 The exocyst is an evolutionarily conserved octameric protein complex that mediates the tethering of post-Golgi secretory vesicles to the plasma membrane during exocytosis and is implicated in many cellular processes such as cell polarization, cytokinesis, ciliogenesis and tumor invasion. Using cryo-EM and chemical cross-linking MS (CXMS), we solved the
Kunrong Mei +12 more
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Cryo-EM data processing and analysis of the RcsF-LolCDE.
, 2022 (A) Representative cryo-EM micrograph of RcsF-LolCDE in nanodisc. (B) Selected 2D class averages of cryo-EM particle images. (C) Scheme of 3D classification and refinement of cryo-EM particle images. (D) Gold-standard FSC curves calculated with different
Yihua Huang (245089) +6 more
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Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
Cryo-EM of nucleosome core particle interactions in trans
Scientific Reports, 2018 Nucleosomes, the basic unit of chromatin, are repetitively spaced along DNA and regulate genome expression and maintenance. The long linear chromatin molecule is extensively condensed to fit DNA inside the nucleus.
Silvija Bilokapic +2 more
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The Current Revolution in Cryo-EM [PDF]
Biophysical Journal, 2016 Structural biology is the study of the molecular architecture of proteins and nucleic acids, which are the basis for all life forms. Structural biology came into its own as a field during the 1950s when the atomic structures of DNA (1) and several globular proteins (2) were solved.
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Expanding capabilities and infrastructure for cryo-EM [PDF]
Nature Methods, 2021 Many challenges and considerations must be evaluated when expanding and supporting new cryo-electron microscopy facilities.
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Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Nature CommunicationsMycobacteria harbor a proteasome that was acquired by Actinobacteria through horizontal gene transfer and that supports the persistence of the human pathogen Mycobacterium tuberculosis within host macrophages. The core particle of the proteasome (20S CP)
Tatjana von Rosen +7 more
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