Results 81 to 90 of about 6,328,527 (298)
The Triplex-Centric Assembly and Maturation of the Herpesvirus Procapsid
VirusesHerpesviruses are prevalent infectious agents in humans, with complex structures and life cycles. The viability and detail of a model of capsid assembly and maturation can now be examined against the recently available mature herpesvirus capsids ...
J. Bernard Heymann
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A cryo-electron microscopic approach to elucidate protein structures from human brain microsomes
Life Science Alliance, 2023 Using cryo-EM to simultaneously identify and solve structures of five enzymes from a raw human brain microsomal lysate, highlighting the potential for facilitating structural-omics at the atomic level.
Marios L Tringides +4 more
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Cryo-EM data processing and analysis of apo-LolCDE.
, 2022 (A) Representative cryo-EM micrograph of LolCDE in nanodisc. (B) Selected 2D class averages of cryo-EM particle images. (C) Scheme of 3D classification and refinement of cryo-EM particle images. (D) Gold-standard Fourier shell correlation (FSC) curves of
Yihua Huang (245089) +6 more
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FEBS Letters, EarlyView.Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley +1 more source
IUCrJCryoEM reconstructions must be performed along the Ewald spheres to achieve resolutions beyond the projection approximation limit. The linear theory of image formation models the scattering from the specimen and focusing by the objective lens as two ...
J. Bernard Heymann
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Atomic cryo-EM structures of viruses [PDF]
Current Opinion in Structural Biology, 2017 During the development of single particle cryo-EM in past five decades, icosahedral viruses have led the resolution progress owing to their large mass and high symmetry. Many technical advances in cryo-EM were first established with viruses. Since reaching ∼4Å resolution in 2008, it has become a relatively routine task to solve the atomic structure of ...
Wen, Jiang, Liang, Tang
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Cryo-EM data processing and analysis of AMPPNP-LolCDE.
, 2022 (A) Representative cryo-EM micrograph of AMPPNP-LolCDE in nanodisc. (B) Selected 2D class averages of cryo-EM particle images. (C) Scheme of 3D classification and refinement of cryo-EM particle images.
Yihua Huang (245089) +6 more
core +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Cryo-EM advances in RNA structure determination
Signal Transduction and Targeted Therapy, 2022 Cryo-electron microscopy (cryo-EM) has emerged as an unprecedented tool to resolve protein structures at atomic resolution. Structural insights of biological samples not accessible by conventional X-ray crystallography and NMR can be explored with cryo ...
Haiyun Ma +3 more
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Cryo-EM data collection, refinement, and validation statistics.
, 2022 Cryo-EM data collection, refinement, and validation statistics.
Hideo Fukuhara (494318) +15 more
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