Results 81 to 90 of about 99,841 (293)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Raman Microscopy at the Organic–Inorganic Interfaces in Human Calcified Aortic Valves Shows the Co‐Existence of Whitlockite Crystals and Carbonated Hydroxyapatite‐Mineralized Collagen Fibrils

Advanced Functional Materials, EarlyView.
This study advances our understanding of aortic valve stenosis by capturing spatially resolved chemical and structural changes at the nanoscale. The findings highlight the potential of combined Raman and electron microscopy for understanding calcification mechanisms across diverse tissue types.
Robin H. M. Van der Meijden, Carolien T. A Kuster, Amber van Broekhoven, Raphaela Allgayer, Luco Rutten, Rona Roverts, Marta Cerruti, Elena Macías‐Sánchez, Jan Hein Cornel, Salloua el Messaoudi, Anat Akiva, Nico Sommerdijk   +11 more
wiley   +1 more source

A labeled dataset for AI-based cryo-EM map enhancement

Computational and Structural Biotechnology Journal
Cryogenic electron microscopy (cryo-EM) has transformed structural biology by enabling near atomic resolution imaging of macromolecular complexes. However, cryo-EM density maps suffer from intrinsic noise arising from structural sources, shot noise, and ...
Nabin Giri, Xiao Chen, Liguo Wang, Jianlin Cheng   +3 more
doaj   +1 more source

A deep convolutional neural network approach to single-particle recognition in cryo-electron microscopy

BMC Bioinformatics, 2017
Background Single-particle cryo-electron microscopy (cryo-EM) has become a mainstream tool for the structural determination of biological macromolecular complexes.
Yanan Zhu, Qi Ouyang, Youdong Mao
doaj   +1 more source

pH-dependent gating mechanism of the Helicobacter pylori urea channel revealed by cryo-EM. [PDF]

, 2019
The urea channel of Helicobacter pylori (HpUreI) is an ideal drug target for preventing gastric cancer but incomplete understanding of its gating mechanism has hampered development of inhibitors for the eradication of H. pylori. Here, we present the cryo-
Cui, Yanxiang, Munson, Keith, Sachs, George, Strugatsky, David, Wen, Yi, Zhou, Kang, Zhou, Z Hong   +6 more
core   +1 more source

Deciphering Small Molecule Diffusion Parameters Across Light Responsive Polymersome Membranes

Advanced Functional Materials, EarlyView.
Light‐responsive polymersomes bearing donor–acceptor Stenhouse adducts (DASAs) enable programmable control over small‐molecule transport across synthetic membranes. By systematically varying DASA density, an optimal functionalization regime is identified that maximizes light‐gated permeability.
Farzina Matubbar, Beyzanur Kaya, Buse Ipek, Tenzin Dorjee, Shoghik Hakobyan, Mohamed Chami, Julien E. Gautrot, Omar Rifaie‐Graham   +7 more
wiley   +1 more source

Fast multiscale reconstruction for Cryo-EM

Journal of Structural Biology, 2018
We present a multiscale reconstruction framework for single-particle analysis (SPA). The representation of three-dimensional (3D) objects with scaled basis functions permits the reconstruction of volumes at any desired scale in the real-space. This multiscale approach generates interesting opportunities in SPA for the stabilization of the initial ...
Donati, Laurène, Nilchian, Masih, Sorzano, Carlos Oscar S., Unser, Michael   +3 more
openaire   +2 more sources

Recent advances in our understanding of the structure and function of more unusual cation channels. [PDF]

, 2019
As their name implies, cation channels allow the regulated flow of cations such as sodium, potassium, calcium, and magnesium across cellular and intracellular membranes.
Brown, Brandon M, Nguyen, Hai M, Wulff, Heike   +2 more
core   +1 more source

From Clinic to Computation: Multiscale Bioengineering Strategies for Durable Biological Aortic Valve Replacements

Advanced Functional Materials, EarlyView.
Bioprosthetic aortic valves have revolutionized the treatment of aortic stenosis, but their durability is limited by structural valve deterioration (SVD). This review focuses on the pericardial tissue at the heart of these valves, examining how its mechanical properties and calcification drive fatigue and failure.
Gabriele Greco, Pierfrancesco Gaziano, Serena Anglese, Simone Morganti, Carlo de Vincentiis, Ferdinando Auricchio, Michele Marino, Michele Conti   +7 more
wiley   +1 more source

A large expert-curated cryo-EM image dataset for machine learning protein particle picking

Scientific Data, 2023
Cryo-electron microscopy (cryo-EM) is a powerful technique for determining the structures of biological macromolecular complexes. Picking single-protein particles from cryo-EM micrographs is a crucial step in reconstructing protein structures.
Ashwin Dhakal, Rajan Gyawali, Liguo Wang, Jianlin Cheng   +3 more
doaj   +1 more source