Results 101 to 110 of about 6,313,615 (343)
Bioinspired Hydrogels with Broadly Programmable Mechanics for Soft‐Tissue Interfaces
Advanced Functional Materials, EarlyView.Sea cucumber‐inspired gelatin hydrogels are formed by directional freezing and mechanically programmed through subsequent ionic treatment. Multiscale characterization and modeling link ion‐regulated chain interactions to directional load transfer within the aligned hierarchical architecture.
Youchao Teng +20 more
wiley +1 more source
In Situ Studies of Mitochondrial Translation by Cryo-Electron Tomography
, 2021 Cryo-electron tomography (cryo-ET) enables the three-dimensional (3D) visualization of macromolecular complexes in their native environment (in situ).
Englmeier, Robert +3 more
core
Advanced Functional Materials, EarlyView.Here, we establish a low‐dose liquid‐phase electron microscopy workflow for real‐time observation of biological processes in hydrated conditions. Beam damage is reduced by imaging only 20% of the pixels and reconstructing the complete image using an inpainting algorithm.
Luco Rutten +7 more
wiley +1 more source
Unraveling atomic complexity from frozen samples
Structural DynamicsCryo-electron microscopy (cryo-EM) is a significant driver of recent advances in structural biology. Cryo-EM is comprised of several distinct and complementary methods, which include single particle analysis, cryo-electron tomography, and microcrystal ...
Yasmeen N. Ruma +2 more
doaj +1 more source
Cryo-EM structure of compound 16-MRGPRX1-Gαq complex.
, 2023 (A) Cryo-EM density map of MRGPRX1-Gαq in complex with compound 16. Receptor, compound 16, Gαq, Gβ, Gγ, Nb35, and scFv16 are colored slate, cyan, marine, forest, pink, salmon, and yellow-orange, respectively.
Rui Lv (3923345) +10 more
core +1 more source
Advanced Healthcare Materials, EarlyView.Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj +23 more
wiley +1 more source
Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering
Advanced Materials, EarlyView.Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera +22 more
wiley +1 more source
Proteins, interfaces, and cryo-EM grids [PDF]
Current Opinion in Colloid & Interface Science, 2018 It has become clear that the standard cartoon, in which macromolecular particles prepared for electron cryo-microscopy are shown to be surrounded completely by vitreous ice, often is not accurate. In particular, the standard picture does not include the fact that diffusion to the air-water interface, followed by adsorption and possibly denaturation ...
openaire +4 more sources
Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency
Advanced Materials, EarlyView.As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang +12 more
wiley +1 more source
Cryo-EM and X-ray data collection.
, 2022 (a) Cryo-EM map of ACS122 and ACS114 in complex with the AMC009 SOSIP trimer. Only the Fab variable regions are shown here. (b) Cryo-EM map of ACS122 and ACS114 in complex with AMC009 SOSIP colored by local resolution (Å). (c) Gold-standard Fourier shell
Patricia van der Woude (9323416) +25 more
core +1 more source