Results 11 to 20 of about 55,954 (213)
Cryo‐EM for Small Molecules [PDF]
Current Protocols, 2022 AbstractIn recent years, protein structure analysis using cryo‐electron microscopy(cryo‐EM) has expanded and improved. In this review, we discuss many recent improvements to the field, the problems those improvements hope to solve, and some of the still unanswered questions.
Cabral, Angela +2 more
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Bayesian cryo-EM Refinement [PDF]
Biophysical Journal, 2015 In light of recent high-resolution cryo-electron-microscopy (cryo-EM) structures of large complexes, modeling atom coordinates into cryo-EM densities faces new challenges. Though high-resolution structures provide more data, finding the best-fitting structure with current refinement methods is more difficult with increased resolution, because the used ...
Blau, C. +4 more
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IUCrJ, 2022 The 2022 meeting of the American Crystallographic Association in Portland was an inspiring event, addressing a range of both conventional and emerging themes in structural biology. The increasing emphasis at the conference on methods outside the conventional envelope of crystallography, especially cryo-electron microscopy, is discussed.
Sriram Subramaniam +2 more
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Current Biology, 2018 Eva Nogales introduces cryo-electron microscopy used to visualize macromolecular structures.
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Protein Science, 2016 Peer Reviewed ; http://deepblue.lib.umich.edu/bitstream/2027.42/135551/1/pro3088 ...
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Proteins: Structure, Function, and Bioinformatics, 2021 Abstract Structures of seven CASP14 targets were determined using cryo‐electron microscopy (cryo‐EM) technique with resolution between 2.1 and 3.8 Å. We provide an evaluation of the submitted models versus the experimental data (cryo‐EM density maps) and experimental reference structures built into the maps.
Tristan Cragnolini +2 more
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IUCrJ, 2020 The application of cryo-EM methods to study SARS-CoV-2 proteins provides a convincing demonstration of the power of cryo-EM in the arsenal of structural biology.
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Cryo-EM in drug discovery [PDF]
Biochemical Society Transactions, 2019 Abstract The impact of structural biology on drug discovery is well documented, and the workhorse technique for the past 30 years or so has been X-ray crystallography. With the advent of several technological improvements, including direct electron detectors, automation, better microscope vacuums and lenses, phase plates and ...
Tom Ceska +4 more
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Scientific Video Protocols, 2020 Richard Henderson discusses Jacques Dubochet's, Joachim Frank's and his contributions to the development of electron cryo-microscopy.
Richard Henderson, Mejd Alsari
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Interpreting the cryo-EM map [PDF]
IUCrJ, 2019 Beckers et al. [IUCrJ (2019), 6, 18-33] propose a general approach to visualization of cryo-EM maps. Their method overcomes some of the challenges inherent in the conventional approach for depicting maps using a single isosurface threshold.
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