Results 11 to 20 of about 6,328,527 (298)
Cryo‐electron microscopy of cholinesterases, present and future [PDF]
, 2021 Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) exist in a variety of oligomeric forms, each with defined cellular and subcellular distributions.
Leung, Miguel Ricardo +3 more
core +3 more sources
Cryo‐EM for Small Molecules [PDF]
Current Protocols, 2022 AbstractIn recent years, protein structure analysis using cryo‐electron microscopy(cryo‐EM) has expanded and improved. In this review, we discuss many recent improvements to the field, the problems those improvements hope to solve, and some of the still unanswered questions.
Cabral, Angela +2 more
openaire +3 more sources
cryo-EM data collection and refinement statistics.
, 2023 cryo-EM data collection and refinement statistics.
Sun Meng (11007860) +8 more
core +4 more sources
Best practice: setting up and operating a mid-sized cryo-EM facility
Frontiers in Molecular Biosciences, 2023 Ever since the resolution revolution in 2013, cryo-electron microscopy (cryo-EM) has become a powerful methodology in structural biology that is especially suited to study the structure of large flexible molecular complexes.
Xing Meng +8 more
doaj +1 more source
TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages
Scientific Reports, 2021 Identifying endogenous tissue stem cells remains a key challenge in developmental and regenerative biology. To distinguish and molecularly characterise stem cell populations in large heterogeneous tissues, the combination of cytochemical cell markers ...
Viola Oorschot +3 more
doaj +1 more source
Practices for running a research-oriented shared cryo-EM facility
Frontiers in Molecular Biosciences, 2022 The Harvard Cryo-Electron Microscopy Center for Structural Biology, which was formed as a consortium between Harvard Medical School, Boston Children’s Hospital, Dana-Farber Cancer Institute, and Massachusetts General Hospital, serves both academic and ...
Richard M. Walsh +14 more
doaj +1 more source
Cryo-EM data collection, refinement and validation statistics.
, 2023 Cryo-EM data collection, refinement and validation statistics.
Quentin Durieux Trouilleton (16792991) +10 more
core +7 more sources
The Ewald sphere/focus gradient does not limit the resolution of cryoEM reconstructions
Journal of Structural Biology: X, 2023 In our quest to solve biomolecular structures to higher resolutions in cryoEM, care must be taken to deal with all aspects of image formation in the electron microscope.
J. Bernard Heymann
doaj +1 more source
BRCA1-A and BRISC: Multifunctional Molecular Machines for Ubiquitin Signaling
Biomolecules, 2020 The K63-linkage specific deubiquitinase BRCC36 forms the core of two multi-subunit deubiquitination complexes: BRCA1-A and BRISC. BRCA1-A is recruited to DNA repair foci, edits ubiquitin signals on chromatin, and sequesters BRCA1 away from the site of ...
Julius Rabl
doaj +1 more source
Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states
Nature Communications, 2023 Herpesviruses remain a burden for animal and human health, including the medically important varicella-zoster virus (VZV). Membrane fusion mediated by conserved core glycoproteins, the fusogen gB and the heterodimer gH-gL, enables herpesvirus cell entry.
Momei Zhou +7 more
doaj +1 more source