Results 11 to 20 of about 59,949 (213)

Accelerating discovery and rational engineering of antibody modalities using Cryo- EM [PDF]

Structural Dynamics
As the need for novel therapeutic modalities is growing, researchers require innovative strategies and transformative technologies to overcome the limitations of traditional antibody discovery methodologies.
Tilak Gupta, Surajit Banerjee
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A Bayesian approach to extracting free-energy profiles from cryo-electron microscopy experiments

Scientific Reports, 2021
Cryo-electron microscopy (cryo-EM) extracts single-particle density projections of individual biomolecules. Although cryo-EM is widely used for 3D reconstruction, due to its single-particle nature it has the potential to provide information about a ...
Julian Giraldo-Barreto, Sebastian Ortiz, Erik H. Thiede, Karen Palacio-Rodriguez, Bob Carpenter, Alex H. Barnett, Pilar Cossio   +6 more
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Integrative approaches in cryogenic electron microscopy: Recent advances in structural biology and future perspectives

iScience, 2021
Summary: Cellular factories engage numerous highly complex “molecular machines” to perform pivotal biological functions. 3D structural visualization is an effective way to understand the functional mechanisms of these biomacromolecules.
Aneek Banerjee, Sayan Bhakta, Jayati Sengupta   +2 more
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A Visualization Tool for Cryo-EM Protein Validation with an Unsupervised Machine Learning Model in Chimera Platform

Medicines, 2019
Background: Cryo-electron microscopy (cryo-EM) has become a major technique for protein structure determination. However, due to the low quality of cryo-EM density maps, many protein structures derived from cryo-EM contain outliers introduced during the ...
Lin Chen, Brandon Baker, Eduardo Santos, Michell Sheep, Darius Daftarian   +4 more
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Evolving data standards for cryo-EM structures

Structural Dynamics, 2020
Electron cryo-microscopy (cryo-EM) is increasingly being used to determine 3D structures of a broad spectrum of biological specimens from molecules to cells. Anticipating this progress in the early 2000s, an international collaboration of scientists with
Catherine L. Lawson, Helen M. Berman, Wah Chiu   +2 more
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Molecular Dynamics to Predict Cryo-EM: Capturing Transitions and Short-Lived Conformational States of Biomolecules

Frontiers in Molecular Biosciences, 2021
Single-particle cryogenic electron microscopy (cryo-EM) has revolutionized the field of the structural biology, providing an access to the atomic resolution structures of large biomolecular complexes in their near-native environment. Today’s cryo-EM maps
Łukasz Nierzwicki, Giulia Palermo, Giulia Palermo   +2 more
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Biological Breakthroughs and Drug Discovery Revolution via Cryo-Electron Microscopy of Membrane Proteins

Membranes
The application of cryo-electron microscopy (cryo-EM) in membrane protein structural biology has catalyzed unprecedented advances in our understanding of fundamental biological processes and transformed drug discovery paradigms.
Vitor Hugo Balasco Serrão
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Routine sub-2.5 Å cryo-EM structure determination of GPCRs

Nature Communications, 2021
There is a need to optimise cryo-EM data acquisition approaches to improve the resolution of GPCR cryo-EM structures to better than 2.5 Å, in order to use them for structure-based drug design purposes.
Radostin Danev, Matthew Belousoff, Yi-Lynn Liang, Xin Zhang, Fabian Eisenstein, Denise Wootten, Patrick M. Sexton   +6 more
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Cryogenic electron tomography reveals herpesvirus capsid assembly intermediates inside the cell nucleus

Nature Communications
Herpesviruses encapsulate their double-stranded DNA (dsDNA) genomes within an icosahedral nucleocapsid formed in the infected cell nucleus. Four biochemically purified nucleocapsids have been characterized, but their roles in herpesvirus replication ...
Stefan L. Oliver, Muyuan Chen, Leeya Engel, Corey W. Hecksel, Xueting Zhou, Michael F. Schmid, Ann M. Arvin, Wah Chiu   +7 more
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Using cryo-electron microscopy maps for X-ray structure determination

IUCrJ, 2018
X-ray crystallography and cryo-electron microscopy (cryo-EM) are complementary techniques for structure determination. Crystallography usually reveals more detailed information, while cryo-EM is an extremely useful technique for studying large-sized ...
Lingxiao Zeng, Wei Ding, Quan Hao
doaj   +1 more source