Backprojection-based reconstruction and correction for distance-dependent defocus in cryoelectron microscopy [PDF]
, 2008 We study two methods of micrograph processing in cryoelectron microscopy: the defocus-gradient corrected back-projection algorithm and the correction of micrographs for the contrast transfer function based on the frequency-distance relation.
Laslo Cernetic +5 more
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Bacterial selenocysteine synthase structure revealed by single-particle cryoEM
Current Research in Structural BiologyThe 21st amino acid, selenocysteine (Sec), is synthesized on its dedicated transfer RNA (tRNASec). In bacteria, Sec is synthesized from Ser-tRNA[Ser]Sec by Selenocysteine Synthase (SelA), which is a pivotal enzyme in the biosynthesis of Sec.
Vitor Hugo Balasco Serrão +3 more
doaj +1 more source
口腔疾病防治, 2023 The Porphyromonas gingivalis type IX secretion system (T9SS) is a recently discovered protein secretion system that is widely distributed in Bacillus cereus. The T9SS is structurally complex and powerful.
ZHANG Ruotong, YE Wei
doaj +1 more source
Quaternary organization of a phytochrome dimer as revealed by cryoelectron microscopy. [PDF]
Proc Natl Acad Sci U S A, 2010 Phytochromes are a collection of dimeric photoreceptors that direct a diverse array of responses in plants and microorganisms through photoconversion between a red light-absorbing ground state Pr, and a far-red light-absorbing photoactivated state Pfr.
Li H, Zhang J, Vierstra RD, Li H.
europepmc +4 more sources
The stack: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1(T) by transmission electron microscopy and tomography. [PDF]
PLoS ONE, 2013 In recent years, improvements in transmission electron microscopy (TEM) techniques and the use of tomography have provided a more accurate view of the complexity of the ultrastructure of prokaryotic cells. Cryoimmobilization of specimens by rapid cooling
Lidia Delgado +4 more
doaj +1 more source
Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
Bio-Protocol, 2021 Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production.
Florent Waltz +2 more
doaj +1 more source
, 2001 The structure of the Paired Helical filaments (PHF), a polymer of the microtubule associated protein tau, has been studied by Atomic Force Microscopy (AFM) and by cryoelectron microscopy. Mica and graphite were used as substrates in the AFM analysis with
Jaime Colchero +13 more
core +1 more source
Cryoelectron Microscopy and Image Analysis of the Cardiac Ryanodine Receptor [PDF]
Journal of Biological Chemistry, 1998 The three-dimensional structure of the cardiac muscle ryanodine receptor (RyR2) is described and compared with its skeletal muscle isoform (RyR1). Previously, structural studies of RyR2 have not been as informative as those for RyR1 because optimal conditions for electron microscopy, which require low levels of phospholipid, are destabilizing for RyR2.
M R, Sharma +5 more
openaire +2 more sources
Vitrification of cryoelectron microscopy specimens revealed by high-speed photographic imaging.
, 2003 Cryoelectron microsopy is a widely used technique to observe biological material in an almost physiological, fully hydrated state. The sample is prepared for electron microsopy observation by quickly reducing its temperature to -180 degrees C.
Adrian, M. +4 more
core +1 more source
Fully three-dimensional defocus-gradient corrected backprojection in cryoelectron microscopy. [PDF]
Ultramicroscopy, 2010 Recognizing that the microscope depth of field is a significant resolution-limiting factor in 3D cryoelectron microscopy, Jensen and Kornberg proposed a concept they called defocus-gradient corrected backprojection (DGCBP) and illustrated by computer ...
Kazantsev IG +3 more
europepmc +2 more sources