Morphology of influenza B/Lee/40 determined by cryo-electron microscopy. [PDF]
PLoS ONE, 2014 Cryo-electron microscopy projection image analysis and tomography is used to describe the overall architecture of influenza B/Lee/40. Algebraic reconstruction techniques with utilization of volume elements (blobs) are employed to reconstruct tomograms of
Garrett Katz +9 more
doaj +1 more source
Cryo-electron microscopy of extracellular vesicles from cerebrospinal fluid.
PLoS ONE, 2020 Extracellular vesicles (EVs) are membrane-enclosed vesicles which play important role for cell communication and physiology. EVs are found in many human biological fluids, including blood, breast milk, urine, cerebrospinal fluid (CSF), ejaculate, saliva ...
Anton Emelyanov +10 more
doaj +1 more source
Learning to automate cryo-electron microscopy data collection with Ptolemy
IUCrJ, 2023 Over the past decade, cryo-electron microscopy (cryoEM) has emerged as an important method for determining near-native, near-atomic resolution 3D structures of biological macromolecules.
Paul T. Kim +3 more
doaj +1 more source
Membrane shape as a reporter for applied forces [PDF]
, 2008 Recent advances have enabled 3-dimensional reconstructions of biological structures in vivo, ranging in size and complexity from single proteins to multicellular structures. In particular, tomography and confocal microscopy have been exploited to capture
Antonny +30 more
core +2 more sources
Quaternary organization of a phytochrome dimer as revealed by cryoelectron microscopy. [PDF]
Proc Natl Acad Sci U S A, 2010 Phytochromes are a collection of dimeric photoreceptors that direct a diverse array of responses in plants and microorganisms through photoconversion between a red light-absorbing ground state Pr, and a far-red light-absorbing photoactivated state Pfr.
Li H, Zhang J, Vierstra RD, Li H.
europepmc +4 more sources
Bacterial selenocysteine synthase structure revealed by single-particle cryoEM
Current Research in Structural BiologyThe 21st amino acid, selenocysteine (Sec), is synthesized on its dedicated transfer RNA (tRNASec). In bacteria, Sec is synthesized from Ser-tRNA[Ser]Sec by Selenocysteine Synthase (SelA), which is a pivotal enzyme in the biosynthesis of Sec.
Vitor Hugo Balasco Serrão +3 more
doaj +1 more source
The stack: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1(T) by transmission electron microscopy and tomography. [PDF]
PLoS ONE, 2013 In recent years, improvements in transmission electron microscopy (TEM) techniques and the use of tomography have provided a more accurate view of the complexity of the ultrastructure of prokaryotic cells. Cryoimmobilization of specimens by rapid cooling
Lidia Delgado +4 more
doaj +1 more source
Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
Bio-Protocol, 2021 Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production.
Florent Waltz +2 more
doaj +1 more source
Lensfree super-resolution holographic microscopy using wetting films on a chip. [PDF]
, 2011 We investigate the use of wetting films to significantly improve the imaging performance of lensfree pixel super-resolution on-chip microscopy, achieving < 1 µm spatial resolution over a large imaging area of ~24 mm(2).
Bishara, Waheb +2 more
core +1 more source
Cryoelectron Microscopy and Image Analysis of the Cardiac Ryanodine Receptor [PDF]
Journal of Biological Chemistry, 1998 The three-dimensional structure of the cardiac muscle ryanodine receptor (RyR2) is described and compared with its skeletal muscle isoform (RyR1). Previously, structural studies of RyR2 have not been as informative as those for RyR1 because optimal conditions for electron microscopy, which require low levels of phospholipid, are destabilizing for RyR2.
M R, Sharma +5 more
openaire +2 more sources