Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes
Cell Reports, 2021 Summary: The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism, and appetite and is an important target for treatment of type 2 diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials,
Xin Zhang +5 more
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Correlative light electron microscopy of giant viruses with the SECOM system
New Microbes and New Infections, 2018 Bright-field transmission electron microscopy (TEM), TEM-negative staining technique, resin-embedding and ultramicrotomy, scanning TEM, scanning electron microscopy, atomic force microscopy and cryoelectron microscopy are imaging techniques used for ...
J.-P. Baudoin +6 more
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Recent advances in retroviruses via cryo-electron microscopy
Retrovirology, 2018 Cryo-electron microscopy has undergone a revolution in recent years and it has contributed significantly to a number of different areas in biological research.
Johnson Mak, Alex de Marco
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Morphology of influenza B/Lee/40 determined by cryo-electron microscopy. [PDF]
PLoS ONE, 2014 Cryo-electron microscopy projection image analysis and tomography is used to describe the overall architecture of influenza B/Lee/40. Algebraic reconstruction techniques with utilization of volume elements (blobs) are employed to reconstruct tomograms of
Garrett Katz +9 more
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Cryo-electron microscopy of extracellular vesicles from cerebrospinal fluid.
PLoS ONE, 2020 Extracellular vesicles (EVs) are membrane-enclosed vesicles which play important role for cell communication and physiology. EVs are found in many human biological fluids, including blood, breast milk, urine, cerebrospinal fluid (CSF), ejaculate, saliva ...
Anton Emelyanov +10 more
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Learning to automate cryo-electron microscopy data collection with Ptolemy
IUCrJ, 2023 Over the past decade, cryo-electron microscopy (cryoEM) has emerged as an important method for determining near-native, near-atomic resolution 3D structures of biological macromolecules.
Paul T. Kim +3 more
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Bacterial selenocysteine synthase structure revealed by single-particle cryoEM
Current Research in Structural BiologyThe 21st amino acid, selenocysteine (Sec), is synthesized on its dedicated transfer RNA (tRNASec). In bacteria, Sec is synthesized from Ser-tRNA[Ser]Sec by Selenocysteine Synthase (SelA), which is a pivotal enzyme in the biosynthesis of Sec.
Vitor Hugo Balasco Serrão +3 more
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The stack: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1(T) by transmission electron microscopy and tomography. [PDF]
PLoS ONE, 2013 In recent years, improvements in transmission electron microscopy (TEM) techniques and the use of tomography have provided a more accurate view of the complexity of the ultrastructure of prokaryotic cells. Cryoimmobilization of specimens by rapid cooling
Lidia Delgado +4 more
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Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
Bio-Protocol, 2021 Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production.
Florent Waltz +2 more
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Cryoelectron Microscopy and Image Analysis of the Cardiac Ryanodine Receptor [PDF]
Journal of Biological Chemistry, 1998 The three-dimensional structure of the cardiac muscle ryanodine receptor (RyR2) is described and compared with its skeletal muscle isoform (RyR1). Previously, structural studies of RyR2 have not been as informative as those for RyR1 because optimal conditions for electron microscopy, which require low levels of phospholipid, are destabilizing for RyR2.
M R, Sharma +5 more
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